Expression of flowering locus T2 transgene from Pyrus communis L. delays dormancy and leaf senescence in Malus × domestica Borkh, and causes early flowering in tobacco

Freiman, Aviad; Golobovitch, Sara; Yablovitz, Zeev; Belausov, Eduard; Dahan, Yardena; Peer, Reut; Avraham, Lior; Freiman, Zohar E.; Evenor, Dalia; Reuveni, Moshe; Sobolev, V. M.; Edelman, Marvin; Shahak, Yosepha; Samach, Alon; Flaishman, Moshe A.

doi:10.1016/j.plantsci.2015.09.012

Cited by 32 publications

(43 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Still, cold temperatures might also contribute to release of lateral bud dormancy in olive, and one or both of the FT-encoding genes might be mediating this process as well. There is some evidence that FT-encoding genes have a role in the control of bud dormancy in other perennial species (Hsu et al 2011;Freiman et al 2015). Our finding that the transgenic olives overexpressing MtFTa1 lost apical dominance causing growth of many lateral buds might support such a theory.…”

Section: Discussionmentioning

confidence: 52%

See 1 more Smart Citation

A possible role for flowering locus T‐encoding genes in interpreting environmental and internal cues affecting olive (Olea europaea L.) flower induction

Haberman

Bakhshian

Cerezo-Medina

et al. 2017

Plant Cell & Environment

Self Cite

108

View full text Add to dashboard Cite

Olive (Olea europaea L.) inflorescences, formed in lateral buds, flower in spring. However, there is some debate regarding time of flower induction and inflorescence initiation. Olive juvenility and seasonality of flowering were altered by overexpressing genes encoding flowering locus T (FT). OeFT1 and OeFT2 caused early flowering under short days when expressed in Arabidopsis. Expression of OeFT1/2 in olive leaves and OeFT2 in buds increased in winter, while initiation of inflorescences occurred i n late winter. Trees exposed to an artificial warm winter expressed low levels of OeFT1/2 in leaves and did not flower. Olive flower induction thus seems to be mediated by an increase in FT levels in response to cold winters. Olive flowering is dependent on additional internal factors. It was severely reduced in trees that carried a heavy fruit load the previous season (harvested in November) and in trees without fruit to which cold temperatures were artificially applied in summer. Expression analysis suggested that these internal factors work either by reducing the increase in OeFT1/2 expression or through putative flowering repressors such as TFL1. With expected warmer winters, future consumption of olive oil, as part of a healthy Mediterranean diet, should benefit from better understanding these factors.

show abstract

Section: Discussionmentioning

confidence: 52%

“…; Freiman et al . ). Our finding that the transgenic olives overexpressing MtFTa1 lost apical dominance causing growth of many lateral buds might support such a theory.…”

Section: Discussionmentioning

confidence: 97%

A possible role for flowering locus T‐encoding genes in interpreting environmental and internal cues affecting olive (Olea europaea L.) flower induction

Haberman

Bakhshian

Cerezo-Medina

et al. 2017

Plant Cell & Environment

Self Cite

108

View full text Add to dashboard Cite

show abstract

“…Manipulations of FT and CEN availability and dosage provide means to manipulate developmental mechanisms; overexpression of FT genes (Klocko et al ., ; Srinivasan et al ., ; Wenzel et al ., ) and down‐regulation of TFL1/CEN genes (Freiman et al ., ; Kotoda et al ., ; Yamagishi et al ., ) have been advanced as tools for accelerated breeding. These approaches have often proven to be unreliable (Zhang et al ., ) or failed to promote flowering in fruit crops, affecting other aspects of development instead (Freiman et al ., ; Varkonyi‐Gasic et al ., ). In contrast, stable mutations in these genes have underpinned domestication of crops such as legumes, sugar beet, sunflower and strawberry (Blackman et al ., ; Iwata et al ., ; Kwak et al ., ; Liu et al ., ; Pin et al ., ), or in case of a natural mutation in the CEN homolog SELF PRUNING (Pnueli et al ., ), revolutionized production and industrial processing of tomato.…”

Section: Introductionmentioning

confidence: 99%

Mutagenesis of kiwifruit CENTRORADIALIS‐like genes transforms a climbing woody perennial with long juvenility and axillary flowering into a compact plant with rapid terminal flowering

Varkonyi‐Gasic

Wang

Voogd

et al. 2018

Plant Biotechnology Journal

107

View full text Add to dashboard Cite

Summary Annualization of woody perennials has the potential to revolutionize the breeding and production of fruit crops and rapidly improve horticultural species. Kiwifruit ( Actinidia chinensis ) is a recently domesticated fruit crop with a short history of breeding and tremendous potential for improvement. Previously, multiple kiwifruit CENTRORADIALIS ( CEN )‐like genes have been identified as potential repressors of flowering. In this study, CRISPR /Cas9‐ mediated manipulation enabled functional analysis of kiwifruit CEN ‐like genes Ac CEN 4 and Ac CEN . Mutation of these genes transformed a climbing woody perennial, which develops axillary inflorescences after many years of juvenility, into a compact plant with rapid terminal flower and fruit development. The number of affected genes and alleles and severity of detected mutations correlated with the precocity and change in plant stature, suggesting that a bi‐allelic mutation of either Ac CEN 4 or Ac CEN may be sufficient for early flowering, whereas mutations affecting both genes further contributed to precocity and enhanced the compact growth habit. CRISPR /Cas9‐mediated mutagenesis of Ac CEN 4 and Ac CEN may be a valuable means to engineer Actinidia amenable for accelerated breeding, indoor farming and cultivation as an annual crop.

show abstract

“…Ectopic expression implied distinctive roles in reproductive and vegetative growth for poplar FT paralogs (Hsu et al , 2011), not yet confirmed by paralog-specific silencing; expression of only one of two FT genes in apple and one of three FT genes in citrus has been associated with transition to flowering (Nishikawa et al , 2007; Kotoda et al , 2010), and expression of a single grape FT gene could be detected irrespective of flowering (Sreekantan and Thomas, 2006; Carmona et al , 2007). Furthermore, some FT genes failed to induce early flowering in woody perennials, despite their ability to promote flowering in model annuals, while affecting dormancy and leaf senescence in apple (Freiman et al , 2015) or shoot vigor in kiwifruit (Varkonyi-Gasic et al , 2013). …”

Section: Introductionmentioning

confidence: 99%

Three FT and multiple CEN and BFT genes regulate maturity, flowering, and vegetative phenology in kiwifruit

Voogd

Brian

Wang

et al. 2017

Journal of Experimental Botany

View full text Add to dashboard Cite

show abstract

Expression of flowering locus T2 transgene from Pyrus communis L. delays dormancy and leaf senescence in Malus × domestica Borkh, and causes early flowering in tobacco

Cited by 32 publications

References 49 publications

A possible role for flowering locus T‐encoding genes in interpreting environmental and internal cues affecting olive (Olea europaea L.) flower induction

A possible role for flowering locus T‐encoding genes in interpreting environmental and internal cues affecting olive (Olea europaea L.) flower induction

Mutagenesis of kiwifruit CENTRORADIALIS‐like genes transforms a climbing woody perennial with long juvenility and axillary flowering into a compact plant with rapid terminal flowering

Three FT and multiple CEN and BFT genes regulate maturity, flowering, and vegetative phenology in kiwifruit

Contact Info

Product

Resources

About