Olive (Olea europaea L.) inflorescences, formed in lateral buds, flower in spring. However, there is some debate regarding time of flower induction and inflorescence initiation. Olive juvenility and seasonality of flowering were altered by overexpressing genes encoding flowering locus T (FT). OeFT1 and OeFT2 caused early flowering under short days when expressed in Arabidopsis. Expression of OeFT1/2 in olive leaves and OeFT2 in buds increased in winter, while initiation of inflorescences occurred i n late winter. Trees exposed to an artificial warm winter expressed low levels of OeFT1/2 in leaves and did not flower. Olive flower induction thus seems to be mediated by an increase in FT levels in response to cold winters. Olive flowering is dependent on additional internal factors. It was severely reduced in trees that carried a heavy fruit load the previous season (harvested in November) and in trees without fruit to which cold temperatures were artificially applied in summer. Expression analysis suggested that these internal factors work either by reducing the increase in OeFT1/2 expression or through putative flowering repressors such as TFL1. With expected warmer winters, future consumption of olive oil, as part of a healthy Mediterranean diet, should benefit from better understanding these factors.
SUMMARYIn many perennial fruit trees, flowering in the year following a year with heavy fruit load can be quite limited. This biennial cycle of fruiting, termed alternate bearing, was described 170 years ago in apple (Malus domestica). Apple inflorescences are mainly found on short branches (spurs). Bourse shoots (BS) develop from the leaf axils of the spur. BS apices may terminate~100 days after flowering, with formation of next year's inflorescences. We sought to determine how developing fruit on the spur prevents the adjacent BS apex from forming an inflorescence. The presence of adjacent fruit correlated with reaccumulation of transcript encoding a potential flowering inhibitor, MdTFL1-2, in BS apices prior to inflorescence initiation. BS apices without adjacent fruit that did not flower due to late fruitlet removal, neighbouring fruit on the tree, or leaf removal, also reaccumulated the MdTFL1-2 transcript. Fruit load and gibberellin (GA) application had similar effects on the expression of MdTFL1-2 and genes involved in GA biosynthesis and metabolism. Some apple cultivars are less prone to alternate bearing. We show that the response of a BS apex to different numbers of adjacent fruit differs among cultivars in both MdTFL1-2 accumulation and return flowering. These results provide a working model for the further study of alternate bearing, and help clarify the need for cultivar-specific approaches to reach stable fruit production.
The olive growing sector is transitioning from traditional to intensive irrigated cultivation, dictating a need to reconsider orchard management practices including fertilization. Potassium (K) is an essential nutrient, typically found in high concentrations in plants. Orchard K fertilization requirements are commonly derived from the disparity between assumed tree requirements and extractable soil K. The long-term impact of insufficient fertilization on K available in the soil, growth, and yield of irrigated field-grown olive trees was evaluated over six consecutive seasons. Withholding of K fertilization led to lower exchangeable and soluble K concentrations in the soil and significantly impaired yield. The reduction in yield was attributed to reduced flowering and fruit set, resulting in a lower fruit number. Tree vegetative growth and flowering quality traits were not affected. In addition, trees not receiving K appeared to be more susceptible to alternate bearing. Following two seasons of omitting K fertilization, leaf K concentration did not decrease below the conventionally accepted sufficiency threshold for olive (0.8%). In spite of this, the trees produced significantly lower yields. Our results suggest that long-term insufficient K fertilization results in reduced soil available K and consequently impairs tree productivity. The results imply that the sufficiency threshold for K in diagnostic leaves should be reconsidered for intensive orchards. Moreover, the current method for K deficiency detection using leaf K concentration may be inadequate for intensive orchards. Integration of other parameters, such as fruit K content, leaf Na, and changes in soil exchangeable K content or sorption energy, may promote a more reliable analysis of orchard K nutritional status.
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