Expression of Fibronectin and Tenascin after Direct Capping of the Pulp with Mineral Trioxide Aggregate and Biodentine®

Abstract

<jats:p>BACKGROUND: Caries is a destructive process leading to progressive demineralization of the inorganic part of the tooth, accompanied by enzyme disintegration of the organic component of the tooth tissue. Considering the fact that caries activity can be stopped if the demineralization process is stopped, it is clear that the objectives of caries treatment are focused toward elimination of etiological factors and stimulation of regeneration of the dental tissues. That is why in the last years treatment of the caries disease is targeted to remineralization of the initial carious lesions, as well as on the biological behavior of the pulp-dentine complex after application of certain medications.&#x0D; AIM: The aim of this study is to evaluate impact of the two materials for direct capping, mineral trioxide aggregate (MTA) and Biodentine®, on the expression of glycoproteins fibronectin (FN), and tenascin (TN), responsible for dentinogenesis.&#x0D; METHODS: In the tests were used materials MTA and Biodentine, as agents for direct capping of pulp exposure. From 60 extracted teeth included in this in vitro study, tissue cuts were made. Each of them was then analyzed on a light microscope to determine the amount of two extracellular matrix glycoproteins, FN-C, and TN-C.&#x0D; RESULTS: Our study show that there is an expressed immunoreactivity for FN and for TN in the fibronectin bridge under MTA and Biodentine® after 8, and even after 30 days of their application.&#x0D; CONCLUSION: Based on this study, we can conclude that both materials – MTA and Biodentine – may induce reparative dentinogenesis, in which FN and TN have certainly a major role in the formation of the fibronectin matrix.</jats:p>

Expression of a fibronectin in the dental pulp of lead intoxicated rats with experimentally induced diabetes mellitus

“…Fibronectin represents one of the most significant glycoproteins which has a major influence on the odontogenesis process. It maintains the connection between cells and components of the extracellular matrix in healthy and infected tissues; affects the migration and polarization of odontoblasts and is considered to be a trigger for differentiation of odontoblasts [18][19][20][21].…”

“…High expression of fibronectin was noted in pulp cells after non-specific inductive effects of certain factors, such as bacterial and mechanical factors, on the pulp resulting in the formation of a fibronectin-rich matrix. This matrix serves as a source of growth factors, which are considered to be signalling molecules for odontoblast differentiation which may explain fibronectin expression in the control group of rats [19,20,21].…”