Expression of extracellular matrix proteins in cervical squamous cell carcinoma--a clinicopathological study.

Goldberg, Iris; Davidson, Ben; Lerner‐Geva, Liat; Gotlieb, Walter H.; Ben‐Baruch, Gilad; Novikov, Ilya; Kopolovic, Juri

doi:10.1136/jcp.51.10.781

Cited by 16 publications

(15 citation statements)

References 20 publications

(19 reference statements)

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“…4, 5, and 6, the 481 cm −1 band is not observed in the Raman spectra of normal cervical squamous cells, and therefore a strong contribution by glycogen to the squamous cell spectra is unlikely. The role of collagen in squamous cell carcinoma has also been reported by Parikka et al31 and Goldberg et al32…”

Section: Resultssupporting

confidence: 58%

Near-Infrared Micro-Raman Spectroscopy for in Vitro Detection of Cervical Cancer

et al. 2010

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Near-infrared Raman spectroscopy is a powerful analytical tool for detecting critical differences in biological samples with minimum interference in the Raman spectra from the native fluorescence of the samples. The technique is often suggested as a potential screening tool for cancer. In this article we report in vitro Raman spectra of squamous cells in normal and cancerous cervical human tissue from seven patients, which have good signal-to-noise ratio and which were found to be reproducible. These preliminary results show that several Raman features in these spectra could be used to distinguish cancerous cervical squamous cells from normal cervical squamous cells. In general, the Raman spectra of cervical cancer cells show intensity differences compared to those of normal squamous cell spectra. For example, several well-defined Raman peaks of collagen in the 775 to 975 cm −1 region are observed in the case of normal squamous cells, but these are below the detection limit of normal Raman spectroscopy in the spectra of invasive cervical cancer cells. In the high frequency 2800 to 3100 cm −1 region, it is found that the peak area under the CH stretching band is lower by a factor of approximately six in the spectra of cervical cancer cells as compared with that of the normal cells. The Raman chemical maps of regions of cancer and normal cells in the cervical epithelium made from the spectral features in the 775 to 975 cm −1 and 2800 to 3100 cm −1 regions are also found to show good correlation with each other.

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Section: Resultssupporting

confidence: 58%

Near-Infrared Micro-Raman Spectroscopy for in Vitro Detection of Cervical Cancer

et al. 2010

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“…The Raman spectrum of glycogen shown in Fig. 4 is consistent with that reported in the literature 23,[31][32][33][34][35] . Similarly, the Raman spectrum of collagen in Fig.…”

Section: Optical Microscopy and Micro-raman Spectroscopy Of Cervical supporting

confidence: 92%

“…2, and 4, the 481 cm -1 band is not observed in the Raman spectra of normal cervical squamous cells, and therefore a strong contribution by glycogen to the squamous cell spectra is unlikely. The role of collagen in squamous cell carcinoma has also been reported by Parikka et al 35 and Goldberg. 36 Figure 5 shows the micro-photographs of cancerous and normal ovarian tissues mounted on polished aluminum substrate with 10x and 50x objectives.…”

Section: Optical Microscopy and Micro-raman Spectroscopy Of Cervical mentioning

confidence: 71%

In-vitro micro-Raman study of tissue samples for detecting cervical and ovarian cancer with 785-nm laser excitation

Sharma¹,

Kamemoto

Misra

et al. 2010

Smart Biomedical and Physiological Sensor Technologies VII

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We present results of in vitro micro-Raman spectroscopy of normal and cancerous cervical and ovarian tissues excited with 785 nm near-infrared (NIR) laser. Micro-Raman spectra of squamous cervical cells of both cervix and ovarian tissues show significant differences in the spectra of normal and cancerous cells. In particular, several well-defined Raman peaks in the 775-975 cm -1 region are observed in the spectra of normal cervix squamous cells but are completely missing in the spectra of invasive cervical cancer cells. In the high-frequency 2800-3100 cm -1 region it is shown that the peak area under CH stretching band is much lower than the corresponding area in the spectra of normal cells. In the case of ovarian tissues, the micro-Raman spectra show noticeable spectral differences between normal cells and ovarian serous cancer cells. In particular, we observed the accumulation of β-carotene in ovarian serous cancer cells compared to normal ovarian cells from women with no ovarian cancer. The NIR micro-Raman spectroscopy offers a potential molecular technique for detecting cervical and ovarian cancer from the respective tissues.

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“…ⅱ) Few studies have investigated the relationship between PAI-1 and collagen IV, while most studies investigated the relationship between PAI-1 and fibronectin, vitronectin and laminin (1,8,11,35). ⅲ) Collagen IV is known to be rich in basal membrane (where cervical cancer occurs) (37), and collagen IV could be important for CSCs. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…1A), it is suggestive that PAI-1 is important for the interaction between cancer cells and basal membrane, considering that laminin is a major component of basal membrane of uterine cervix as well as collagen IV (37).…”

Section: Discussionmentioning

confidence: 99%

Decreased expression of the plasminogen activator inhibitor type 1 is involved in degradation of extracellular matrix surrounding cervical cancer stem cells

Sato

Kawana

Adachi

et al. 2015

International Journal of Oncology

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Abstract. The plasminogen activator (PA) system consists of plasminogen activator inhibitor type 1 (PAI-1), urokinase-type plasminogen activator and its receptor (uPA and uPAR). PAI-1 inhibits the activation of uPA (which converts plasminogen to plasmin), and is involved in cancer invasion and metastasis, by remodeling the extracellular matrix (ECM) through regulating plasmin. Cancer stem cells (CSCs) are a small subset of cells within tumors, and are thought to be involved in tumor recurrence and metastasis. Considering these facts, we investigated the relationship between PAI-1 and cervical CSCs. We used ALDH1 as a marker of cervical CSCs. First, we demonstrated that culturing ALDH1-high cells and ALDH-low cells on collagen IV-coted plates increased their expression of active PAI-1 (ELISA), and these increases were suggested to be at mRNA expression levels (RT-qPCR). Secondly, we demonstrated PAI-1 was indeed involved in the ECM maintenance. With gelatin zymography assays, we found that ALDH1-high cells and ALDH-low cells expressed pro-matrix metalloproteinase-2 (pro-MMP-2) irrespective of their coatings. With gelatinase/collagenase assay kit, we confirmed that collagenase activity was increased when ALDH1-low cells were exposed to TM5275, a small molecule inhibitor of PAI-1. Putting the data together, we hypothesized that cancer cells adhered to basal membrane secrete abundant PAI-1, on the other hand, cancer cells (especially CSCs rather than non-CSCs) distant from basal membrane secrete less PAI-1, which makes the ECM surrounding CSCs more susceptible to degradation. Our study could be an explanation of conflicting reports, where some researchers found negative impacts of PAI-1 expression on clinical outcomes and others not, by considering the concept of CSCs.

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Expression of extracellular matrix proteins in cervical squamous cell carcinoma--a clinicopathological study.

Cited by 16 publications

References 20 publications

Near-Infrared Micro-Raman Spectroscopy for in Vitro Detection of Cervical Cancer

Near-Infrared Micro-Raman Spectroscopy for in Vitro Detection of Cervical Cancer

In-vitro micro-Raman study of tissue samples for detecting cervical and ovarian cancer with 785-nm laser excitation

Decreased expression of the plasminogen activator inhibitor type 1 is involved in degradation of extracellular matrix surrounding cervical cancer stem cells

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