Near-Infrared Micro-Raman Spectroscopy for in Vitro Detection of Cervical Cancer

Abstract

Near-infrared Raman spectroscopy is a powerful analytical tool for detecting critical differences in biological samples with minimum interference in the Raman spectra from the native fluorescence of the samples. The technique is often suggested as a potential screening tool for cancer. In this article we report in vitro Raman spectra of squamous cells in normal and cancerous cervical human tissue from seven patients, which have good signal-to-noise ratio and which were found to be reproducible. These preliminary results show that several Raman features in these spectra could be used to distinguish cancerous cervical squamous cells from normal cervical squamous cells. In general, the Raman spectra of cervical cancer cells show intensity differences compared to those of normal squamous cell spectra. For example, several well-defined Raman peaks of collagen in the 775 to 975 cm −1 region are observed in the case of normal squamous cells, but these are below the detection limit of normal Raman spectroscopy in the spectra of invasive cervical cancer cells. In the high frequency 2800 to 3100 cm −1 region, it is found that the peak area under the CH stretching band is lower by a factor of approximately six in the spectra of cervical cancer cells as compared with that of the normal cells. The Raman chemical maps of regions of cancer and normal cells in the cervical epithelium made from the spectral features in the 775 to 975 cm −1 and 2800 to 3100 cm −1 regions are also found to show good correlation with each other.

Near-infrared-excited confocal Raman spectroscopy advancesin vivodiagnosis of cervical precancer

“…For instance, the spectra showed decreased glycogen and structural proteins (854 cm −1 ), primarily representing the loss of epithelial cell differentiation during neoplastic progression. 22,32 The increased nuclear content (1095 cm −1 ) indicated the elevated number of epithelial cells in the dysplastic cervix. 22,32,33 The amide I (1654 cm −1 ) and amide III (1253 cm −1 ) bands were also noticeably decreased with dysplastic transformation.…”

“…1(e) 7,10,[20][21][22] The comprehensive biomolecular basis of Raman spectroscopic diagnosis of cervical dysplasia has been described in detail elsewhere. 7,9,22 The AF spectra show higher intensity for dysplasia; however, the change in AF intensity associated with dysplastic progression was not significant (p ¼…”

Raman Spectroscopy of Ghost Cells in Calcifying Cystic Odontogenic Tumor

“…Therefore, Raman spectroscopy is used for various researches [1][2][3][4][5][6][7][8][9] , suggesting Raman spectroscopic analysis is a powerful and useful tool. There are some advantages to using Raman spectroscopy for analysis of water-rich and multi- The present study demonstrated the micro FT-Raman ability for obtaining the spectra of ghost cells in CCOT and keratinocytes of the gingiva.…”

Raman spectroscopy for screening and diagnosis of cervical cancer

“…The loss of differentiation, together with increased proliferation, in precancer results in reduced levels of glycogen, as normal cervical cells accumulate glycogen as they mature. A Raman mapping study using frozen tissue sections showed that Raman spectroscopy could distinguish normal cervical tissue from invasive cervical cancer tissue based mainly on collagen bands and CH stretching bands [41].Tan et al [42] used Raman spectral mapping and hierarchical cluster analysis (HCA) to differentiate between normal squamous epithelium and CIN2 in FFPP tissue sections and it was shown that the Raman spectra associated with the CIN2 lesion clustered predominantly with those of the basal epithelial cells of the normal squamous epithelium, suggesting that the cells of these regions share common biochemical profiles. Spectral features responsible for their differentiation were associated with the amide I and amide III bands.…”