Expression of epidermal growth factor receptor in oral and esophageal squamous-cell carcinoma

Shahsavari, Fatemeh; Miri, Rosa; Ghorbanpour, Maedeh

doi:10.4103/1735-3327.280890

Cited by 8 publications

(5 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When analyzing mEGFR in relation to the investigated clinicopathologic features of OSCC, we also did not find a single statistically significant correlation. Shahsavari failed to demonstrate any correlation between mEGFR expression and clinicopathologic features of OSCC, consistent with our findings [ 84 ]. In contrast, Costa et al demonstrated the negative impact of EGFR on disease progression in individuals younger than 40 years, which contradicts our observations [ 85 ].…”

Section: Discussionsupporting

confidence: 92%

Nuclear Epidermal Growth Factor Receptor Overexpression as a Survival Predictor in Oral Squamous Cell Carcinoma

Tarle

Raguž

Müller

et al. 2023

IJMS

View full text Add to dashboard Cite

The aim of this study was to determine, by immunohistochemical methods, the expression of nEGFR and markers of cell proliferation (Ki-67), cell cycle (mEGFR, p53, cyclin D1), and tumor stem cells (ABCG2) in 59 pathohistological samples of healthy oral mucosa, 50 oral premalignant changes (leukoplakia and erythroplakia), and 52 oral squamous cell carcinomas (OSCC). An increase in the expression of mEGFR and nEGFR was found with the development of the disease (p < 0.0001). In the group of patients with leukoplakia and erythroplakia, we found a positive correlation between nEGFR and Ki67, p53, cyclin D1, and mEGFR, whereas in the group of patients with OSCC, we found a positive correlation between nEGFR and Ki67, mEGFR (p < 0.05). Tumors without perineural (PNI) invasion had a higher expression of p53 protein than tumors with PNI (p = 0.02). Patients with OSCC and overexpression of nEGFR had shorter overall survival (p = 0.004). The results of this study suggest a potentially important independent role of nEGFR in oral carcinogenesis.

show abstract

Section: Discussionsupporting

confidence: 92%

Nuclear Epidermal Growth Factor Receptor Overexpression as a Survival Predictor in Oral Squamous Cell Carcinoma

Tarle

Raguž

Müller

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Recent studies have also demonstrated the noticeable overexpression of EGFR in oral cancer cells [30]. It seems that the overexpression of EGFR in OCCs depends on the tumor stage, invasion, metastasis to the lymph nodes, distant metastasis, and differentiation of cancer [28,31]. Considering the epithelial origin of most cancers, EGFR expression deserves to be considered in the cancer treatment processes.…”

Section: Discussionmentioning

confidence: 99%

“…Our study determined that curcumin, the Bilhar extract, and their combination could decrease the expression of the EGFR gene and increase the apoptosis rate in cancerous cells. This protein, from the ErbB family, is a tyrosine kinase receptor involved in numerous processes affecting tumor growth, progression, differentiation, invasion, metastasis, and apoptosis inhibition [28,29]. Recent studies have also demonstrated the noticeable overexpression of EGFR in oral cancer cells [30].…”

Section: Discussionmentioning

confidence: 99%

Nanoliposomal Coencapsulation of Dorema aucheri Extract and Curcumin; Enhanced Cytotoxicity, Apoptosis Induction, and Inhibition of EGFR Gene Expression in Oral Cancer Cells OCC-02

Azizi,

Ghalamfarsa,

Khosravani

et al. 2023

IET Nanobiotechnology

View full text Add to dashboard Cite

Curcumin is one of the natural anticancer drugs but its efficiency is limited by low stability, insufficient bioavailability, poor solubility, and poor permeability. Dorema aucheri (Bilhar) is a herb with precious pharmaceutical properties. This study aimed to develop a nanoliposome-based curcumin and Bilhar extract codelivery system. The nanocompounds were synthesized using the lipid thin-film hydration method and characterized by transmission electron microscopy, and dynamic light scattering techniques, and their cytotoxicity and apoptotic effect on the primary oral cancer cell line were evaluated via 2,5-diphenyl-2H-tetrazolium bromide assay and flow cytometry. Moreover, the expression of the epidermal growth factor receptor (EGFR) gene in the treated cells was assessed using the real-time polymerase chain reaction technique. Based on the results, nanoliposomes had a size of 91 ± 10 nm with a polydispersity index of 0.13. Free curcumin, the extract, and the curcumin-extract combination showed dose-dependent toxicity against cancer cells; yet, the extract (IC50: 86 µg/ml) and curcumin-extract (IC50: 65 µg/ml) activities were much more than curcumin (IC50: 121 µg/ml). Also, the curcumin and extract loaded on liposomes showed a dose and time-dependent cytotoxicity. After loading the curcumin-extract compound on nanoliposomes, their IC50 decreased from 180 µg/ml (within 24 hr) to 43 µg/ml (within 72 hr), indicating their sustainable release and activity. Likewise, this compound induced the highest apoptosis percentage (95%) in cancerous cells and inhibited the expression of the EGFR gene in the cells by 81% ± 3%. These findings demonstrated the effectiveness of the Bilhar extract against oral cancer cells. Also, in combination with curcumin, it showed an additive activity that considerably improved after loading on nanoliposomes.

show abstract

“…Positive expression of EGFR has already been reported for oral squamous cell carcinoma, representing the most common type of malignancy in the oral cavity and esophagus [ 40 , 41 ]. Therefore, the EGFR Affibody–IR700Dye conjugate holds therapeutic potential for targeting these tumors as well.…”

Section: Discussionmentioning

confidence: 99%

Near-Infrared Photoimmunotherapy Using a Protein Mimetic for EGFR-Positive Salivary Gland Cancer

Yamaguchi,

Suzuki,

Okada

et al. 2024

IJMS

View full text Add to dashboard Cite

Near-infrared photoimmunotherapy (NIR-PIT) is a novel cancer therapy based on a monoclonal antibody (mAb) conjugated to a photosensitizer (IR700Dye). The conjugate can be activated by near-infrared light irradiation, causing necrotic cell death with high selectivity. In this study, we investigated NIR-PIT using a small protein mimetic (6–7 kDa, Affibody) which has more rapid clearance and better tissue penetration than mAbs for epidermal growth factor receptor (EGFR)-positive salivary gland cancer (SGC). The level of EGFR expression was examined in vitro using immunocytochemistry and Western blotting. Cell viability was analyzed using the alamarBlue assay. In vivo, the volume of EGFR-positive tumors treated with NIR-PIT using the EGFR Affibody–IR700Dye conjugate was followed for 43 days. It was found that NIR-PIT using the EGFR Affibody–IR700Dye conjugate induced the selective destruction of EGFR-positive SGC cells and restricted the progression of EGFR-positive tumors. We expect that NIR-PIT using the EGFR Affibody–IR700Dye conjugate can efficiently treat EGFR-positive SGC and preserve normal salivary function.

show abstract

Expression of epidermal growth factor receptor in oral and esophageal squamous-cell carcinoma

Cited by 8 publications

References 18 publications

Nuclear Epidermal Growth Factor Receptor Overexpression as a Survival Predictor in Oral Squamous Cell Carcinoma

Nuclear Epidermal Growth Factor Receptor Overexpression as a Survival Predictor in Oral Squamous Cell Carcinoma

Nanoliposomal Coencapsulation of Dorema aucheri Extract and Curcumin; Enhanced Cytotoxicity, Apoptosis Induction, and Inhibition of EGFR Gene Expression in Oral Cancer Cells OCC-02

Near-Infrared Photoimmunotherapy Using a Protein Mimetic for EGFR-Positive Salivary Gland Cancer

Contact Info

Product

Resources

About