Expression of EpCam and Villin in Barrett’s Esophagus and in Gastric Cardia

Anders, Mario; Sarbia, Mario; Grötzinger, Carsten; Meining, Alexander; Höfler, Heinz; Wiedenmann, Bertram; Rösch, Thomas

doi:10.1155/2008/679893

Cited by 9 publications

(5 citation statements)

References 34 publications

(47 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First, we performed immunofluorescence to evaluate the EpCAM expression from SQ, BE, HGD, and EAC in TMA. The previous literature 9,30 has reported that EpCAM is a marker of BE and EAC. However, there is little evidence for comparisons among BE, HGD, EAC.…”

Section: ■ Discussionmentioning

confidence: 99%

Identification of Tumor Specific Peptide as EpCAM Ligand and Its Potential Diagnostic and Therapeutic Clinical Application

Kang

et al. 2019

Mol. Pharmaceutics

View full text Add to dashboard Cite

Tumor targeting agents are being developed for early tumor detection and therapeutics. We previously identified the peptide SNFYMPL (SNF*) and demonstrated its specific binding to human esophageal specimens of high-grade dysplasia (HGD) and adenocarcinoma with imaging ex vivo. Here, we aim to identify the target for this peptide and investigate its potential applications in imaging and drug delivery. With SNF* conjugated affinity chromatography, mass spectrum, Western blot, enzyme-linked immunosorbent assay (ELISA), and molecular docking, we found that the epithelial cell adhesion molecule (EpCAM) was the potential target of SNF*. Next, we showed that FITC-labeled SNF* (SNF*-FITC) colocalized with EpCAM antibody on the surface of esophageal adenocarcinoma cells OE33, and SNF*-FITC binding patterns significantly changed after EpCAM knockdown or exogenous EpCAM transfection. With the data from TCGA, we demonstrated that EpCAM was overexpressed in 17 types of cancers. Using colon and gastric adenocarcinoma cells and tissues as examples, we found that SNF*-FITC bound in a pattern was colocalized with EpCAM antibody, and the SNF* binding did not upregulate the EpCAM downstream Wnt signals. Subsequently, we conjugated SNF* with our previously constructed poly(histidine)-PEG/DSPE copolymer micelles. SNF* labeling significantly improved the micelle binding with colon and gastric adenocarcinoma cells in vitro, and enhanced the antitumor effects and decreased the toxicities of the micelles in vivo. In conclusion, we identified and validated SNF* as a specific peptide for EpCAM. The future potential use of SNF* peptide in multiple tumor surveillance and tumor-targeted therapeutics was demonstrated.

show abstract

Section: ■ Discussionmentioning

confidence: 99%

Identification of Tumor Specific Peptide as EpCAM Ligand and Its Potential Diagnostic and Therapeutic Clinical Application

Kang

et al. 2019

Mol. Pharmaceutics

View full text Add to dashboard Cite

show abstract

“…Although many hypotheses concerning the pathogenesis of GOJ mucosal metaplasia have been proposed, the precise molecular mechanisms of metaplastic change at the GOJ mucosa and the origin of cells from which GOJ metaplastic mucosa forms are not clear. The elevated expression of CDX2, EpCam and villin have been reported at the human GOJ metaplastic mucosa 213 214 249–252. However, increases in these markers were shown in the cardiac-type columnar metaplasia and may be used as predictors for the future development of IM.…”

Section: Resultsmentioning

confidence: 97%

Kyoto international consensus report on anatomy, pathophysiology and clinical significance of the gastro-oesophageal junction

Sugano

Spechler

El-Omar

et al. 2022

Gut

View full text Add to dashboard Cite

ObjectiveAn international meeting was organised to develop consensus on (1) the landmarks to define the gastro-oesophageal junction (GOJ), (2) the occurrence and pathophysiological significance of the cardiac gland, (3) the definition of the gastro-oesophageal junctional zone (GOJZ) and (4) the causes of inflammation, metaplasia and neoplasia occurring in the GOJZ.DesignClinical questions relevant to the afore-mentioned major issues were drafted for which expert panels formulated relevant statements and textural explanations.A Delphi method using an anonymous system was employed to develop the consensus, the level of which was predefined as ≥80% of agreement. Two rounds of voting and amendments were completed before the meeting at which clinical questions and consensus were finalised.ResultsTwenty eight clinical questions and statements were finalised after extensive amendments. Critical consensus was achieved: (1) definition for the GOJ, (2) definition of the GOJZ spanning 1 cm proximal and distal to the GOJ as defined by the end of palisade vessels was accepted based on the anatomical distribution of cardiac type gland, (3) chemical and bacterial (Helicobacter pylori) factors as the primary causes of inflammation, metaplasia and neoplasia occurring in the GOJZ, (4) a new definition of Barrett’s oesophagus (BO).ConclusionsThis international consensus on the new definitions of BO, GOJ and the GOJZ will be instrumental in future studies aiming to resolve many issues on this important anatomic area and hopefully will lead to better classification and management of the diseases surrounding the GOJ.

show abstract

“…Finally, EpCAM is also highly expressed in Barrett’s esophagus. 42 Thus, we examined staining for EpCAM in comparison with MUC4 in the first gland (Fig. 6).…”

Section: Resultsmentioning

confidence: 99%

“…It is also notable that MUC4 and EpCAM are strongly expressed in Barrett's epithelium. [38][39][40]42,52,53 The first gland has also been suggested as a target for graft versus host disease. 54 In mouse models, the first gland has also been implicated in the generation of neoplastic lineages.…”

Section: Discussionmentioning

confidence: 99%

Unique Cellular Lineage Composition of the First Gland of the Mouse Gastric Corpus

O’Neil

Petersen

Choi

et al. 2016

J Histochem Cytochem.

View full text Add to dashboard Cite

SummaryThe glandular stomach has two major zones: the acid secreting corpus and the gastrin cell-containing antrum. Nevertheless, a single gland lies at the transition between the forestomach and corpus in the mouse stomach. We have sought to define the lineages that make up this gland unit at the squamocolumnar junction. The first gland in mice showed a notable absence of characteristic corpus lineages, including parietal cells and chief cells. In contrast, the gland showed strong staining of Griffonia simplicifolia-II (GSII)-lectin-positive mucous cells at the bases of glands, which were also positive for CD44 variant 9 and Clusterin. Prominent numbers of doublecortin-like kinase 1 (DCLK1) positive tuft cells were present in the first gland. The first gland contained Lgr5-expressing putative progenitor cells, and a large proportion of the cells were positive for Sox2. The cells of the first gland stained strongly for MUC4 and EpCAM, but both were absent in the normal corpus mucosa. The present studies indicate that the first gland in the corpus represents a unique anatomic entity. The presence of a concentration of progenitor cells and sensory tuft cells in this gland suggests that it may represent a source of reserve reparative cells for adapting to severe mucosal damage. (J Histochem Cytochem 65:47-58, 2017)

show abstract

Expression of EpCam and Villin in Barrett’s Esophagus and in Gastric Cardia

Cited by 9 publications

References 34 publications

Identification of Tumor Specific Peptide as EpCAM Ligand and Its Potential Diagnostic and Therapeutic Clinical Application

Identification of Tumor Specific Peptide as EpCAM Ligand and Its Potential Diagnostic and Therapeutic Clinical Application

Kyoto international consensus report on anatomy, pathophysiology and clinical significance of the gastro-oesophageal junction

Unique Cellular Lineage Composition of the First Gland of the Mouse Gastric Corpus

Contact Info

Product

Resources

About