Expression of Enzymes During the Germination of Seeds in Endangered Cerrado Species

Abstract

The objective of this study was to evaluate the enzymes expression during the seeds germination process of ever-lasting species Comanthera elegans and Comanthera bisulcata. For the evaluation of the seeds physiological potential, the germination test and index of germination speed were performed. The expression of enzymes esterase (EST), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), superoxide dismutase (SOD), catalase (CAT) and endo-β-mannanase during the germination process were evaluated. The expression of these enzymes was evaluated in dried seeds, in the protrusion, in the emergence of the primal leaf, at the beginning of the formation of normal seedling and dormant seeds at the end of the germination process. To the extent that the germination process occurs in the species C. bisulcata and C. elegans there is greater expression of the enzyme CAT and lower of the enzyme EST. There is variation in the expression of the enzymes SOD, ADH and MDH in seeds of both species during the germination process. The enzyme endo-β-mannanase presents greater activity in seeds with radicle protrusion in the two studied species.

Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds

“…During the coffee seed germination process, protein hydration, respiration, cell elongation, and structural and enzymatic changes are reported before root protrusion (Selmar et al, 2006), as well as endosperm weakening which accompany further embryo development (Walters;Coffee Science, 15:e151704, 2020 Moroni, 2017). With respect to the enzymatic changes in the role of dehydrogenase malate (MDH) and superoxide dismutase (SOD) are highlighted (Assis et al, 2019). The enzyme malate dehydrogenase catalyzes the last reaction of the Krebs cycle (Tunes et al, 2011), being able to transfer electrons from their substrates to generate reduced NAD (P) H or FADH 2 , which then produce ATP via mitochondrial electron transport chain (He et al, 2019).…”