Expression of dentin matrix protein 1 (DMP1) in nonmineralized tissues

Terasawa, Makiko; Shimokawa, Reiko; Terashima, Tatsuo; Ohya, Keiichi; Takagi, Yasuaki; Shimokawa, Hitoyata

doi:10.1007/s00774-004-0504-4

Cited by 75 publications

(69 citation statements)

References 37 publications

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“…The expression of dentin matrix protein 1 (DMP1), which is also a member of the SIBLING group, has already been established in non-mineralized tissues (Terasawa et al 2004). Studies in our lab have also proved the expression of SIBLING proteins in the rat articular cartilage ).…”

Section: Discussionmentioning

confidence: 82%

Expression of Dentin Sialophosphoprotein in Non-mineralized Tissues

Prasad

Zhu

Sun

et al. 2011

J Histochem Cytochem.

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SummaryDentin sialophosphoprotein (DSPP) and its cleaved products, dentin phosphoprotein (DPP) and dentin sialoprotein (DSP), play important roles in biomineralization. Believed to be tooth specific, the authors' group revealed its expression in bone, and more recently, they and other groups also showed its expression in a few types of soft tissues. In this study, the authors systematically examined the expression of DSPP in a variety of non-mineralized tissues using reverse-transcription polymerase chain reaction (RT-PCR), real-time PCR, Western immunoblotting, and immunohistochemistry analyses in wildtype mice as well as β-galactosidase assays in the Dspp lacZ knock-in mice. These approaches showed the presence of DSPP in the salivary glands, cartilage, liver, kidney, and brain and its absence in the heart and spleen. Real-time PCR showed that the expression levels of DSPP mRNA in salivary glands, cartilage, liver, and kidney were higher than in the bone. Interestingly, DSPP was observed in the pericytes of blood vessels in the dental pulp, which are believed to be able to differentiate into odontoblasts. On the basis of these observations, the authors conclude that DSPP and/or its cleaved products may fulfill important functions in certain non-mineralized tissues in addition to its role in biomineralization. (J Histochem Cytochem 59:1009-1021, 2011

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Section: Discussionmentioning

confidence: 82%

Expression of Dentin Sialophosphoprotein in Non-mineralized Tissues

Prasad

Zhu

Sun

et al. 2011

J Histochem Cytochem.

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“…(18) Our finding of a significant decrease of AR expression in the quadriceps muscle can be attributed to DMP1 expression in muscle, however with no significant changes in skeletal muscle weight. (29) Interestingly, although age-related trabecular bone loss in mice affects both genders, males appear to be less affected than females, (30) suggesting that androgens may protect male mice against age-related bone loss. (27,30) Both in our current experiments and in previous studies in mice, systemic androgen activity as assessed by seminal vesicle weight/body weight (21) did not decrease between 12 and 32 weeks of age.…”

Section: Discussionmentioning

confidence: 99%

Androgen receptor (AR) in osteocytes is important for the maintenance of male skeletal integrity: Evidence from targeted AR disruption in mouse osteocytes

Sinnesael

Claessens

Laurent

et al. 2012

Journal of Bone and Mineral Research

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Androgens play a key role in the maintenance of male skeletal integrity. The regulation of this integrity by androgen receptor (AR) signaling has been mainly attributed to osteoblasts. Although osteocytes have emerged as key regulators of bone remodeling, the influence of sex steroids on these cells has been poorly studied. We aimed to investigate the role of AR signaling, specifically in osteocytes using the Cre/LoxP system in male mice (driven by dentin matrix protein 1 [ocy-ARKOs]). Osteocyte fractions of control (AR(ex2)/Y) and ocy-ARKO (ARflox(ex2)/Y; DMP1-cre) mice isolated through sequential collagenase digestion showed increasing AR expression toward the mature osteocyte fraction of control males compared with the more immature fractions, whereas this was reduced by >80% in ocy-ARKO osteocytes. The skeletal phenotype of mutant mice was further assessed by histomorphometry and quantitative micro-computed tomography at 12 and 32 weeks of age. Ocy-ARKOs had significantly lower trabecular bone volume and number in femora and tibias at 32 weeks as well as decreased trabecular number in the L 5 vertebra at 12 weeks. Biomechanical testing showed that ocy-ARKO femora were also stiffer and required a lower ultimate force to induce failure at 32 weeks. However, femoral cortical structure was not significantly different at any time point. The absence of AR in osteocyte also did not appear to affect trabecular bone formation nor its response to mechanical loading. In conclusion, selective inactivation of the AR in osteocytes of male mice accelerates age-related deterioration of skeletal integrity. These findings provide evidence for a direct role of androgens in the maintenance of trabecular bone through actions of the AR in osteocytes. ß

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“…The protein is assumed to have some role in tissue mineralization based on its chemical properties and presence in the extracellular matrix of dentine and bone (Butler and Ritchie, 1995;Feng et al, 2003). However, mRNA transcripts and the protein itself have also been detected in a wide range of soft tissues, where the gene presumably has other functions (Terasawa et al, 2004). Nucleotide sequence data from DMP1 exon 6 have been analyzed in several previous studies of mammalian intraordinal relationships (e.g., Van Den Bussche et al, 2003;Reeder and Bradley, 2004;Jansa et al, 2006).…”

Section: Dentin Matrix Proteinmentioning

confidence: 99%