Expression of DcPRP1 Is Linked to Carrot Storage Root Formation and Is Induced by Wounding and Auxin Treatment

Ebener, W; Fowler, Thomas J.; Suzuki, Hitoshi; Shaver, John R.; Tierney, Mary L.

doi:10.1104/pp.101.1.259

Cited by 29 publications

(11 citation statements)

References 28 publications

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“…Auxin is known to be a hormonal regulator of adventitious root formation in stem cuttings (Jarvis 1986). Consistent with this, some PRP genes involved in lateral root formation or root hair production show positive regulation of mRNA expression by auxin supply (Ebener et al 1993;Neuteboom et al 1999;Bernhardt & Tierney 2000). Nevertheless, we did not observe a significant effect of IAA treatment on the VvPRP expression in grape cuttings.…”

Section: Discussionsupporting

confidence: 84%

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Molecular identification of proline‐rich protein genes induced during root formation in grape (Vitis vinifera L.) stem cuttings

Thomas

Lee

Schiefelbein

2003

Plant Cell & Environment

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Vegetative reproduction relies on the initiation of new plant organs in response to environmental changes. The rapid formation of roots, and ultimately whole plants, from stem cuttings of grape ( Vitis vinifera L.) provides a useful system to investigate the physiological and molecular basis of organ initiation during vegetative reproduction. In the present study the differential RNA display technique was employed to identify two genes, VvPRP1 and VvPRP2 , that are induced in stem cuttings of grape during rooting. Each of these genes encodes a distinct type of proline-rich protein that is related to different groups of putative cell wall proteins, and their expression is rapidly induced in stem segments within 6 h after severing. Further, each gene's transcript becomes most concentrated in the basal portion of the stem segment in the region of new root formation. Induction of these genes is not significantly enhanced by indole-3-acetic acid (IAA) treatment, and the expression of the VvPRP1 gene, but not the VvPRP2 gene, is wound-inducible. These results suggest that these VvPRP genes play an important role in the initiation of new roots on grape stem cuttings, perhaps by altering the cell wall mechanical properties to enable root emergence.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 52%

Molecular identification of proline‐rich protein genes induced during root formation in grape (Vitis vinifera L.) stem cuttings

Thomas

Lee

Schiefelbein

2003

Plant Cell & Environment

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“…These results do not support a role of PAT or of auxin homeostasis in proline accumulation during AR formation in Petunia cuttings. This is in contrast with the observations that auxin application increased the levels of proline in mung bean hypocotyls (Kuraishi et al 1967) and transcripts of proline-rich proteins (PRPs) involved in lateral root formation in Arabidopsis and carrot (Ebener et al 1993; Neuteboom et al 1999; Bernhardt and Tierney 2000). However, it has been suggested that auxin acts as a regulator of PRP genes in a particular time- and concentration-dependent manner (Thomas et al 2003).…”

Section: Discussionmentioning

confidence: 78%

Distribution of indole-3-acetic acid in Petunia hybrida shoot tip cuttings and relationship between auxin transport, carbohydrate metabolism and adventitious root formation

Ahkami

Melzer

Ghaffari

et al. 2013

Planta

140

115

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To determine the contribution of polar auxin transport (PAT) to auxin accumulation and to adventitious root (AR) formation in the stem base of Petuniahybrida shoot tip cuttings, the level of indole-3-acetic acid (IAA) was monitored in non-treated cuttings and cuttings treated with the auxin transport blocker naphthylphthalamic acid (NPA) and was complemented with precise anatomical studies. The temporal course of carbohydrates, amino acids and activities of controlling enzymes was also investigated. Analysis of initial spatial IAA distribution in the cuttings revealed that approximately 40 and 10 % of the total IAA pool was present in the leaves and the stem base as rooting zone, respectively. A negative correlation existed between leaf size and IAA concentration. After excision of cuttings, IAA showed an early increase in the stem base with two peaks at 2 and 24 h post excision and, thereafter, a decline to low levels. This was mirrored by the expression pattern of the auxin-responsive GH3 gene. NPA treatment completely suppressed the 24-h peak of IAA and severely inhibited root formation. It also reduced activities of cell wall and vacuolar invertases in the early phase of AR formation and inhibited the rise of activities of glucose-6-phosphate dehydrogenase and phosphofructokinase during later stages. We propose a model in which spontaneous AR formation in Petunia cuttings is dependent on PAT and on the resulting 24-h peak of IAA in the rooting zone, where it induces early cellular events and also stimulates sink establishment. Subsequent root development stimulates glycolysis and the pentose phosphate pathway.Electronic supplementary materialThe online version of this article (doi:10.1007/s00425-013-1907-z) contains supplementary material, which is available to authorized users.

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“…The extensin protein from N. plumbaginifolia does not display these [Tyr]3 blocks although it contains a considerable amount of tyrosine (18.5%) arranged at TPVYK and KKPYYPPH repeats (De Loose et al 1991). The failure to induce extensin gene expression to high levels in leaves upon wounding might be explained by the fact that the expression in healthy leaves is very low, and regulatory mechanisms involved in the synthesis of extensin may require the presence of an already-established developmental program (Ebener et al 1993). Tomato extensin gene expression, which is high in stems and absent in leaves, can only be enhanced in stems and not in leaves by wounding (Showalter and Varner 1987).…”

Section: Discussionmentioning

confidence: 94%

Extensin gene expression is induced by mechanical stimuli leading to local cell wall strengthening in Nicotiana plumbaginifolia

Tiré

Rycke

Loose

et al. 1994

Planta

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Nicotiana plumbaginifolia Viv. harbors a single extensin gene, although related hydroxyproline-rich sequences are present in the genome. Northern analysis showed that the gene is highly expressed in roots and to a lesser extent in stems. Expression in leaves is low but mRNA levels are increased upon infection with the incompatible bacterium Pseudomonas syringae. Extensin transcript levels in leaves were slightly enhanced after wounding and salicylic acid treatment. In-situ hybridization experiments showed high accumulation of extensin mRNA in cells which, at certain stages of development, require reinforcement of their cell walls. The cortical cells in stem nodes and roots, which are put under severe mechanical stress by adjacent developing tissues, tend to express the gene to high levels. Immunolocalization of the extensin protein in stems and roots demonstrated a close association of the protein with lignin deposition. Mature tissues contained more extensin than younger tissues. The extensin promoter was fused to the beta-glucuronidase gene.

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Expression of DcPRP1 Is Linked to Carrot Storage Root Formation and Is Induced by Wounding and Auxin Treatment

Cited by 29 publications

References 28 publications

Molecular identification of proline‐rich protein genes induced during root formation in grape (Vitis vinifera L.) stem cuttings

Molecular identification of proline‐rich protein genes induced during root formation in grape (Vitis vinifera L.) stem cuttings

Distribution of indole-3-acetic acid in Petunia hybrida shoot tip cuttings and relationship between auxin transport, carbohydrate metabolism and adventitious root formation

Extensin gene expression is induced by mechanical stimuli leading to local cell wall strengthening in Nicotiana plumbaginifolia

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