Expression of cytoprotective proteins, heat shock protein 70and metallothioneins, in tissues ofOstrea edulis exposed to heat andheavy metals

Piano, Annamaria; Valbonesi, Paola; Fabbri, Elena

doi:10.1379/483.1

Cited by 174 publications

(92 citation statements)

References 46 publications

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“…For example, the expression of HSP70 and HSP90 in oysters exposed to Cd showed a significant enhancement [14,37]. However, no significant difference of MgHSPA12A expression was observed when Cd concentration increased to 50 mg/L.…”

Section: Discussionmentioning

confidence: 99%

The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge

You

Ning²,

Liu³

et al. 2013

Fish & Shellfish Immunology

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a b s t r a c tHeat shock 70 kDa protein 12A (HSPA12A) is an atypical member of HSP70 family, and the translationally controlled tumor protein (TCTP) is a novel HSP with chaperone-like activity. They are both involved in protecting organisms against various stressors. In the present study, the cDNAs of HSPA12A and TCTP (called MgHSPA12A and MgTCTP) were identified from Mytilus galloprovincialis by RACE approaches. The full-length cDNA of MgHSPA12A and MgTCTP encoded a peptide of 491 and 171 amino acids, respectively. Real-time PCR was employed to analyze the tissue distribution and temporal expression of these two genes after bacterial challenge and cadmium (Cd) exposure. It was found that the transcripts of MgHSPA12A and MgTCTP were dominantly expressed in gonad and muscle, respectively. The expression level of MgTCTP at 48 h post Vibrio anguillarum challenge was detected to be significantly up-regulated in hepatopancreas (P < 0.05). As concerned to Cd exposure, 2.0-fold increase of MgHSPA12A expression compared to that of the control was observed at 48 h in 5 mg/L Cd 2þ -treated group, while the expression levels of MgTCTP were significantly decreased after exposed to both 5 and 50 mg/L Cd 2þ for 24 h and 96 h.These results suggested the potential involvement of MgHSPA12A and MgTCTP in the mediation of the immune responses and environmental stress in mussels.

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Section: Discussionmentioning

confidence: 99%

The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge

You

Ning²,

Liu³

et al. 2013

Fish & Shellfish Immunology

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“…Furthermore, more recent evidence showed that HSPs are involved in the cell response to most environmental and physiological stressors (reviewed by Wegele et al 2004). This includes studies in molluscs (CruzRodriguez et al 2000;Snyder et al 2001;Boutet et al 2003;Piano et al 2004). Here again, semi-quantitative methods were used to obtain evidence for an increase in various stressing conditions.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional expression levels of cell stress marker genes in the Pacific oyster Crassostrea gigas exposed to acute thermal stress

Farcy

Voiseux

Lebel

et al. 2009

Cell Stress and Chaperones

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During the annual cycle, oysters are exposed to seasonal slow changes in temperature, but during emersion at low tide on sunny summer days, their internal temperature may rise rapidly, resulting in acute heat stress. We experimentally exposed oysters to a 1-h acute thermal stress and investigated the transcriptional expression level of some genes involved in cell stress defence mechanisms, including chaperone proteins (heat shock proteins Hsp70, Hsp72 and Hsp90 (HSP)), regulation of oxidative stress (Cu-Zn superoxide dismutase, metallothionein (MT)), cell detoxification (glutathione S-transferase sigma, cytochrome P450 and multidrug resistance (MDR1)) and regulation of the cell cycle (p53). Gene mRNA levels were quantified by reverse transcription-quantitative polymerase chain reaction and expressed as their ratio to actin mRNA, used as a reference. Of the nine genes studied, HSP, MT and MDR1 mRNA levels increased in response to thermal stress. We compared the responses of oysters exposed to acute heat shock in summer and winter and observed differences in terms of magnitude and kinetics. A larger increase was observed in September, with recovery within 48 h, whereas in March, the increase was smaller and lasted more than 2 days. The results were also compared with data obtained from the natural environment. Though the functional molecule is the protein and information at the mRNA level only has limitations, the potential use of mRNAs coding for cell stress defence proteins as early sensitive biomarkers is discussed.

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“…**P≤ 0.01 when compared to DMSO controls (n=6, ±SEM) Discussion Aquatic organisms exhibit altered HSP expression when exposed to range of stressful conditions including seasonal acclimatisation, climate change (increase and decrease in temperature), competition for space, food availability, wave exposure, heavy metal exposure and laboratory adaptation (Buckley et al 2001;Franzellitti and Fabbri 2005;Helmuth et al 2002;Helmuth and Hofmann 2001;Kefaloyianni et al 2005;Lund et al 2006;Todgham et al 2007;Tomanek and Sanford 2003). For molluscs, HSP-like molecules have been identified in the sea slug, Aplysia californica, the oysters, Crassostrea virginica, O. edulis, and Crassostrea gigas, the mussel, Mytilus galloprovincialis, and the clams Tapes philippinarum and Scapharca inaequivalvis (Kuhl et al 1992;Piano et al 2002Piano et al , 2004Tirard et al 1995). Furthermore, HSP70 genes have been cloned and characterised in the B. glabrata embryonic (Bge) cell line and in M. galloprovincialis (Kourtidis et al 2006a, b;Laursen et al 1997).…”

Section: Hsp70 Protein Reduction May Be Regulated By Effects Of Esps mentioning

confidence: 99%

Larval excretory-secretory products from the parasite Schistosoma mansoni modulate HSP70 protein expression in defence cells of its snail host, Biomphalaria glabrata

Zahoor

Davies²,

Kirk³

et al. 2010

Cell Stress and Chaperones

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Synthesis of heat shock proteins (HSPs) following cellular stress is a response shared by many organisms. Amongst the HSP family, the ∼70 kDa HSPs are the most evolutionarily conserved with intracellular chaperone and extracellular immunoregulatory functions. This study focused on the effects of larval excretory-secretory products (ESPs) from the parasite Schistosoma mansoni on HSP70 protein expression levels in haemocytes (defence cells) from its snail intermediate host Biomphalaria glabrata. S. mansoni larval stage ESPs are known to interfere with haemocyte physiology and behaviour. Haemocytes from two different B. glabrata strains, one which is susceptible to S. mansoni infection and one which is resistant, both showed reduced HSP70 protein levels following 1 h challenge with S. mansoni ESPs when compared to unchallenged controls; however, the reduction observed in the resistant strain was less marked. The decline in intracellular HSP70 protein persisted for at least 5 h in resistant snail haemocytes only. Furthermore, in schistosome-susceptible snails infected by S. mansoni for 35 days, haemocytes possessed approximately 70% less HSP70. The proteasome inhibitor, MG132, partially restored HSP70 protein levels in ESP-challenged haemocytes, demonstrating that the decrease in HSP70 was in part due to intracellular degradation. The extracellular signal-regulated kinase (ERK) signalling pathway appears to regulate HSP70 protein expression in these cells, as the mitogen-activated protein-ERK kinase 1/2 (MEK1/2) inhibitor, U0126, significantly reduced HSP70 protein levels. Disruption of intracellular HSP70 protein expression in B. glabrata haemocytes by S. mansoni ESPs may be a strategy employed by the parasite to manipulate the immune response of the intermediate snail host.

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Expression of cytoprotective proteins, heat shock protein 70and metallothioneins, in tissues ofOstrea edulis exposed to heat andheavy metals

Cited by 174 publications

References 46 publications

The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge

The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge

Transcriptional expression levels of cell stress marker genes in the Pacific oyster Crassostrea gigas exposed to acute thermal stress

Larval excretory-secretory products from the parasite Schistosoma mansoni modulate HSP70 protein expression in defence cells of its snail host, Biomphalaria glabrata

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