Expression of cytokeratins in odontogenic jaw cysts: monoclonal antibodies reveal distinct variation between different cyst types

Abstract

Immunostaining with monoclonal antibodies was used to study and compare the cytokeratin content of odontogenic cysts and normal gingival epithelium. Two monoclonal antibodies, PKK2 and KA1, stained the whole epithelium in all cyst samples. In gingivu, PKK2 gave a suprabasal staining and KA1 reacted with all epithelial cell layers. Antibodies PKK1, KM 4.62 and Ks 8.12 gave a heterogeneous staining in follicular and radicular cysts. In keratocysts and in gingiva PKK1 and KM 4.62 reacted mainly with basal cells and Ks 8.12 gave a suprabasal staining. Antibodies reacting with the simple epithelial cytokeratin polypeptide No. 18 (PKK3, Ks 18.18) recognized in gingiva only solitary cells compatible with Merkel cells. In a case of follicular ameloblastoma a distinct staining of tumor epithelium was revealed with these antibodies. In 2 follicular cysts, but not in other cyst types, a layer of cytokeratin 18‐positive cells was revealed. KA5 and Kk 8.60 antibodies, reacting exclusively with keratinizing epithelia, including normal gingiva, gave no reaction in radicular cysts, keratocysts and ameloblastoma. Two of the follicular cysts, were negative for PKK3 and Ks 18.18, but reacted strongly with KA5 and Kk 8.60. The present results show that odontogenic jaw cysts have distinct differences in their cytokeratin content. With the exception of some follicular cysts, they lack signs of keratinizing epithelial differentiation. Only follicular cysts appear to share with some types of ameloblastoma the expression of cytokeratin polypeptide No. 18.

Immunohistochemical comparative study of the odontogenic keratocysts and other odontogenic lesions

“…Odontogenic keratocysts show a particular tendency to recur after their surgical removal that could be due to a multilocular nature or a presence of daughter or satellite cysts that are left behind. However, it has also been hypothesized that the epithelium of these cysts could have an intrinsic potential to growth, resembling benign neoplasms (7). Ameloblastomas are tumors that arise from rests of the dental lamina.…”

Expression of cytokeratin 10, 16 and 17 as biomarkers differentiating odontogenic keratocysts from dentigerous cysts

“…In other research studies using monoclonal antibodies LH2 and LH3, CK 10 was not observed in OKCs 18 . In 1987, Hormia et al 19 reported that CK 10 was not observed in OKCs but was observed in 2 out of 18 cases of dentigerous cysts because a different type of antibody was used for cytokeratin. Note, however, that some research studies showed different results even when the same antibody (LH3, LH2) was used for CK 10 in the epithelial tissue of OKCs [18][19][20]28 .…”

“…In 1987, Hormia et al 19 reported that CK 10 was not observed in OKCs but was observed in 2 out of 18 cases of dentigerous cysts because a different type of antibody was used for cytokeratin. Note, however, that some research studies showed different results even when the same antibody (LH3, LH2) was used for CK 10 in the epithelial tissue of OKCs [18][19][20]28 . In this research, CK 10 was observed in the suprabasal layer, with much higher frequency and intensity of expression in OKCs than dentigerous cysts.…”

“…There are 20 types of cytokeratin, and their expression and distribution are determined by the type and layer of intraoral epithelial cell. Generally, cytokeratin is regarded as the best indicator of epithelial differentiation; the comparison of expression of cytokeratins between OKCs and other odontogenic cysts or soft tissue tumor did not always yield the same results 11,[15][16][17][18][19][20][21] . Therefore, this study was conducted to evaluate the expression of CK 10, 16 and 17 in OKCs and dentigerous cysts, provide information for immunohistochemical research on the cytokeratin of OKCs, and evaluate whether cytokeratins can be a useful indicator for the differential diagnosis of dentigerous cysts.…”

Immunohistochemical comparison of cyclin D1 expression in amelobelastoma, odontogenic keratocyst and radicular cyst

“…Odontogenickeratocysts show a particular tendency to recur after their surgical removal that could be due to a multilocular nature or a DOI: http://dx.doi.org/10.22192/ijcrcps.2016.03.09.008 presence of daughter or satellite cysts that are left behind. However, it has also been hypothesized that the epithelium of these cysts could have an intrinsic potential to growth, resembling benign neoplasms (2). Amelobelastomas are tumors that arise from rests of the dental lamina.…”