Immunohistochemical comparative study of the odontogenic keratocysts and other odontogenic lesions

Abstract

Objectives: The present study was undertaken to compare the pattern of expression of EGFR, cyclin D1, Ki-67, p-53 and carcinoembryonic antigen (CEA) in the epithelial lining of odontogenic keratocysts, dentigerous cysts, radicular cysts and ameloblastomas. Methods: four micrometers, formalin-fixed, paraffin-embedded tissue sections from 11 odontogenic keratocysts, 10 dentigerous cysts, 10 radicular cysts and 10 ameloblastomas were immunohistochemically studied. Results: There were statistically significant differences between cyclin D1 expression in odontogenic keratocysts and radicular cysts (p= 0.001) and ameloblastomas (p= 0.04). The differences in CEA expression between the four studied lesions were statistically significant (p< 0.0005). Proliferating cells were significantly more prevalent in odontogenic keratocysts (p< 0.0005) with a mean percentage of Ki-67 positively stained nuclei of 40%. In dentigerous cysts this mean was of 17%, of 15.5% in RC and of 7.8 in ameloblastomas. Conclusion: Some of these findings could support the theory that odontogenic keratocysts are neoplastic in origin, but other results clearly support that these lesions are developmental cysts with some neoplastic properties because of the high intrinsic growth potential.

Immunohistochemical comparison of cyclin D1 expression in amelobelastoma, odontogenic keratocyst and radicular cyst

“…That could probably be related with the biological aggressiveness of AB and OKCs. Results of AB and OKC's relation differs from what Juan-Carlos de-Vicente et al (12) found. This difference is likely to be explained by differences in staining process.…”

“…The overexpression of this protein shows accelerated G1 progression entering in the S-phase of cell cycle, with lower cell dependence on growth factors for proliferation. Cyclin D1 may play an important role in tumorigenesis and it has been detected in malignant tumors but also in benign neoplasms (12).…”

“…We scored the cyclin D1 expression according to three categories: negative (absence of staining) and positive, and this latter into two additional categories, focal or diffuse staining (12).…”

Odontogenic epithelial proliferation is correlated with COX-2 expression in dentigerous cyst and ameloblastoma

“…Similarly, when IPO-38 was used as a proliferation marker, it was found that the labeling index of IPO-38 was higher in ameloblastomas than in dentigerous cysts (49). However, another study conducted by de Vicente et al demonstrated that Ki-67 expression was higher in dentigerous cysts than in ameloblastomas (50). Furthermore, dentigerous cyst have been found to have significantly higher counts for the proliferative activity marker nucleolar organizer regions than unicystic ameloblastoma (51).…”

Investigation of interleukin-1 alpha and interleukin-6 expression and interleukin-1 alpha gene polymorphism in keratocystic odontogenic tumors and ameloblastomas

“…Partial maxillectomy or mandibulectomy is the most common treatment choice with safety margin of healthy bone to avoid recurrence (6). World Health Organization (WHO) working group recommends the term of keratocystic odontogenic tumor which stresses the neoplastic nature of the entity instead of odontogenic keratocyst for parakeratinized variants (5,7,8). A more "innocent" entity ortokeratinized odontogenic keratocyst (OKK) is not included in this novel classification, is still classified in developmental odontogenic cysts (5,9).…”