Expression of cytokeratin 14 in ameloblast-lineage cells of the developing tooth of rat, both in vivo and in vitro

CCAAT/Enhancer-binding Protein δ (C/EBPδ) Maintains Amelogenin Expression in the Absence of C/EBPα in Vivo

“…The search for a promoter useful for driving Cre recombinase in ectoderm-derived ameloblasts identified the keratin 14 (K14) promoter (12)(13)(14)(15). Therefore, we used the K14 promoter to drive Cre-mediated recombination of floxed C/EBP␣ loci predicted to result in the loss of amelogenin expression.…”

Amelogenin Interacts with Cytokeratin-5 in Ameloblasts during Enamel Growth

“…Lossof-function "mutations" of ATMP correlated well with the loss of amelogenin-ligand interaction. A GMp motif is found in the N-terminal region of Type I cytokeratin 14 (K14), a differentiation marker for ameloblasts prior to amelogenin synthesis (7). The purified or recombinant amelogenin and TRAP bind to K14 in vitro dosimetrically (8).…”

Introduction of a three-dimensional and layered (TDL) culture, a novel primary co-culture method for ameloblasts and pulp-derived cells

“…In a primary culture, the ameloblast-lineage cells after inoculation generally form a monolayer sheet of closely packed cells of cobblestone-like profiles. These cells express cell/differentiation markers c-Met, cytokeratin 14, amelogenins (Tabata et al, 1996b), and ameloblastin (DenBesten et al, 1998) in a manner similar to ameloblasts in vivo. In a primary culture, however, the enamel organ-derived cells soon lose their characteristic cell structures --such as tall columnar, cuboidal, or stellate profiles of the functional cells in the enamel organ --and become flat in a monolayer, whereas these cells in tooth germ organ culture systems retain their original structures and function.…”

“…The primary culture of enamel epithelial cells has been attempted by many investigators using enamel organderived cells from porcine (Limeback 1987, DenBesten et al, 1998, mouse (Chen et al, 1992;Suzawa et al, 2006), rat (Kukita et al, 1992;Tabata et al, 1996bTabata et al, , 2003Matsumura et al, 1998) and human (DenBesten et al, 2005;Yan et al, 2006) tooth germs under different conditions. In a primary culture, the ameloblast-lineage cells after inoculation generally form a monolayer sheet of closely packed cells of cobblestone-like profiles.…”

“…We subjected the TDL gels thus prepared to floating cultures and found that, in the layered epithelial cells, those facing the 3D gel became cuboidal/short columnar in shape, showed cell Introduction of a three-dimensional and layered (TDL) culture, a novel primary co-culture method for ameloblasts and pulp-derived cells sheets were excised from the labial side of the mandibular incisors according to Kukita et al (1992), while the dental pulp tissues were carefully prepared from the mandibular first molars. Each tissue thus obtained was handled according to Tabata et al (1996b) with several modifications as follows. Briefly, the tissue fragments were minced in the Hanks' BSS, treated with 0.25% collagenase (Wako Pure Chemical, Osaka) -200 U/ml DNase I (Roche, Mannheim, Germany, ref.…”