“…The primary culture of enamel epithelial cells has been attempted by many investigators using enamel organderived cells from porcine (Limeback 1987, DenBesten et al, 1998, mouse (Chen et al, 1992;Suzawa et al, 2006), rat (Kukita et al, 1992;Tabata et al, 1996bTabata et al, , 2003Matsumura et al, 1998) and human (DenBesten et al, 2005;Yan et al, 2006) tooth germs under different conditions. In a primary culture, the ameloblast-lineage cells after inoculation generally form a monolayer sheet of closely packed cells of cobblestone-like profiles.…”