Expression of cVg1 mRNA during chicken embryonic development

Somi, Semir; Houweling, Arjan C.; Buffing, Anita A.M.; Moorman, Antoon F.M.; Hoff, Maurice J.B. van den

doi:10.1002/ar.a.10070

Cited by 7 publications

(6 citation statements)

References 20 publications

(24 reference statements)

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“…ALK4 expression in the retina of the embryonic rat eye has been reported previously and described in more detail (Yamada et al, 1999). With regard to expression in the otic placode, no requirement has been identified for ALK4 in ear morphogenesis, although studies do indicate not only that TGFβs are expressed in the ear but also that TGFβ signaling is necessary in promoting its normal development (Ito et al, 2001; Chang et al, 2002; Somi et al, 2003). The finding that ALK4 additionally is expressed in the bilateral heart fields is consistent with the expression of Vg1 in the linear heart tube of the chick (Somi et al, 2003) and raises the possibility of yet another role for ALK4 signaling—cardiogenesis—in vertebrate embryogenesis.…”

Section: Discussionmentioning

confidence: 99%

Developmental analysis of activin‐like kinase receptor‐4 (ALK4) expression in Xenopus laevis

Chen

Ramsdell

2004

Developmental Dynamics

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The type I transforming growth factor-beta (TGF␤) receptor, activin-like kinase-4 (ALK4), is an important regulator of vertebrate development, with roles in mesoderm induction, primitive streak formation, gastrulation, dorsoanterior patterning, and left-right axis determination. To complement previous ALK4 functional studies, we have analyzed ALK4 expression in embryos of the frog, Xenopus laevis. Results obtained with reverse transcriptase-polymerase chain reaction indicate that ALK4 is present in both the animal and vegetal poles of blastula stage embryos and that expression levels are relatively constant amongst embryos examined at blastula, gastrula, neurula, and early tail bud stages. However, the tissue distribution of ALK4 mRNA, as assessed by whole-mount in situ hybridization, was found to change over this range of developmental stages. In the blastula stage embryo, ALK4 is detected in cells of the animal pole and the marginal zone. During gastrulation, ALK4 is detected in the outer ectoderm, involuting mesoderm, blastocoele roof, dorsal lip, and to a lesser extent, in the endoderm. At the onset of neurulation, ALK4 expression is prominent in the dorsoanterior region of the developing head, the paraxial mesoderm, and midline structures, including the prechordal plate and neural folds. Expression in older neurula stage embryos resolves to the developing brain, somites, notochord, and neural crest; thereafter, additional sites of ALK4 expression in tail bud stage embryos include the spinal cord, otic placode, developing eye, lateral plate mesoderm, branchial arches, and the bilateral heart fields. Together, these results not only reflect the multiple developmental roles that have been proposed for this TGF␤ receptor but also define spatiotemporal windows in which ALK4 may function to modulate fundamental embryological events.

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Section: Discussionmentioning

confidence: 99%

Developmental analysis of activin‐like kinase receptor‐4 (ALK4) expression in Xenopus laevis

Chen

Ramsdell

2004

Developmental Dynamics

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“…However, some other processes, less evident morphologically but no less important, occur at lateral positions. Examples are the formation of paraxial and lateral mesoderm, including precardiac mesoderm, for which we have no precise landmarks, but could now be detected with such molecular markers as paraxis (Burgess et al, 1995), cVg1 (Somi et al, 2003), Kif5c (Dathe et al, 2004), cSfrp1 (Esteve et al, 2000), Cited 2 (Schlange et al, 2000), Tbx20 (Iio et al, 2001), cNkx-2,5 (Schultheiss et al, 1995), and Mef2 (Goswami et al, 1994). This will…”

Section: Discussionmentioning

confidence: 95%

Morphological and molecular analysis of the early developing chick requires an expanded series of primitive streak stages

López‐Sánchez¹,

Puelles²,

García‐Martínez³

et al. 2005

J. Morphol.

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A detailed analysis of the gastrulating chick embryo was performed using three methods : time-lapse videotaping of embryos in culture, histological semithin sections, and in situ hybridization with 10 mRNA signals expressed during gastrulation. The results suggest that the gene expression pattern of Goosecoid, Hex, Crescent, and Bmp7 may be involved in the axial establishment of the temporal and spatial arrangement of cells forming the prechordal plate endoderm, and that Chordin, cNot1, Noggin, and Brachyury are precocious markers of cells coming from Hensen's node, which contribute to the rostralmost tip of the notochord, its arrowhead, the head process, and, later, the elongating notochord. These results explain several earlier descriptions based only on morphological analyses of the axial mesodermal structures characteristic of the gastrulation stages. The data, carefully observed and compared with the whole-mount observation in time-lapse video, show that the changes in cell populations, movements, and cell differentiation occur step-by-step over a precise temporal range, which requires the establishment of a subdivision of the stages usually employed. Knowledge of new aspects of avian gastrulation, including gene expression patterns, immunocytochemical analyses, and the great number of recent experiments based on microinjections or transplants of groups of cells to analyze processes of induction or regulation, need the support of a precisely defined scheme of primitive streak stages (PS-stages), and a correlation of these stages with other approaches to provide a finer resolution of the staging steps, and thus to facilitate a better understanding of the initial gastrulation period.

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“…Fold differences were calculated by normalising the CT values obtained for each of the gene against chamber-restricted marker genes. We selected myosin , heavy chain 6 , cardiac muscle , alpha ( myh6 ) and ventricular myosin heavy chain ( vmhc ) as chamber-restricted control genes for atrium and ventricle respectively [ 57 , 58 ].…”

Section: Methodsmentioning

confidence: 99%

Chamber Specific Gene Expression Landscape of the Zebrafish Heart

et al. 2016

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The organization of structure and function of cardiac chambers in vertebrates is defined by chamber-specific distinct gene expression. This peculiarity and uniqueness of the genetic signatures demonstrates functional resolution attributed to the different chambers of the heart. Altered expression of the cardiac chamber genes can lead to individual chamber related dysfunctions and disease patho-physiologies. Information on transcriptional repertoire of cardiac compartments is important to understand the spectrum of chamber specific anomalies. We have carried out a genome wide transcriptome profiling study of the three cardiac chambers in the zebrafish heart using RNA sequencing. We have captured the gene expression patterns of 13,396 protein coding genes in the three cardiac chambers—atrium, ventricle and bulbus arteriosus. Of these, 7,260 known protein coding genes are highly expressed (≥10 FPKM) in the zebrafish heart. Thus, this study represents nearly an all-inclusive information on the zebrafish cardiac transcriptome. In this study, a total of 96 differentially expressed genes across the three cardiac chambers in zebrafish were identified. The atrium, ventricle and bulbus arteriosus displayed 20, 32 and 44 uniquely expressing genes respectively. We validated the expression of predicted chamber-restricted genes using independent semi-quantitative and qualitative experimental techniques. In addition, we identified 23 putative novel protein coding genes that are specifically restricted to the ventricle and not in the atrium or bulbus arteriosus. In our knowledge, these 23 novel genes have either not been investigated in detail or are sparsely studied. The transcriptome identified in this study includes 68 differentially expressing zebrafish cardiac chamber genes that have a human ortholog. We also carried out spatiotemporal gene expression profiling of the 96 differentially expressed genes throughout the three cardiac chambers in 11 developmental stages and 6 tissue types of zebrafish. We hypothesize that clustering the differentially expressed genes with both known and unknown functions will deliver detailed insights on fundamental gene networks that are important for the development and specification of the cardiac chambers. It is also postulated that this transcriptome atlas will help utilize zebrafish in a better way as a model for studying cardiac development and to explore functional role of gene networks in cardiac disease pathogenesis.

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Expression of cVg1 mRNA during chicken embryonic development

Cited by 7 publications

References 20 publications

Developmental analysis of activin‐like kinase receptor‐4 (ALK4) expression in Xenopus laevis

Developmental analysis of activin‐like kinase receptor‐4 (ALK4) expression in Xenopus laevis

Morphological and molecular analysis of the early developing chick requires an expanded series of primitive streak stages

Chamber Specific Gene Expression Landscape of the Zebrafish Heart

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