Expression of cupA gene cluster responsible for biofilm formation in Pseudomonas aeruginosa is enhanced against subinhibitory concentration of carbapenems

Deshamukhya, Chandrayee; Saikia, Ranjan; Das, Bhaskar Jyoti; Paul, Deepjyoti; Dhar, Debadatta; Bhattacharjee, Amitabha

doi:10.1016/j.genrep.2021.101427

Gene Reports

2022

DOI: 10.1016/j.genrep.2021.101427

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Expression of cupA gene cluster responsible for biofilm formation in Pseudomonas aeruginosa is enhanced against subinhibitory concentration of carbapenems

Chandrayee Deshamukhya

Ranjan Saikia

Bhaskar Jyoti Das

et al.

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2023

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Cited by 2 publications

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“…Patients with metabolic diseases, hematological diseases, and malignant tumors as well as those who have undergone surgery or have weakened immunity can be easily infected with this bacterium. It can cause bedsore, abscess, suppurative otitis media, bronchitis, pneumonia, and other diseases [ 4 ]. In addition, the pathogen can infect pigs, poultry, mink, and other animals, suggesting that it is a typical zoonotic pathogen [ 2 , 11 , 19 ].…”

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Extracellular polysaccharide of Lactobacillus plantarum enhance immune efficacy of oprH gene recombinant subunit vaccine from Pseudomonas aeruginosa

TIAN,

GONG,

ZHU

et al. 2023

The Journal of Veterinary Medical Science

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To evaluate the immune enhancement effect of the extracellular polysaccharide of Lactobacillus plantarum on oprH recombinant subunit vaccine from Pseudomonas aeruginosa , a recombinant subunit vaccine of oprH (rOprH vaccine) was developed. The EP-rOprH vaccine was prepared with the extracellular polysaccharide of L. plantarum as an adjuvant. Mice were vaccinated with the rOprH and EP-rOprH vaccines, and the outer membrane protein (OMP) and inactivated vaccines were used as controls. The levels of serum antibody, interferon-γ (IFN-γ), interleukin (IL-2), and IL-4 were determined after vaccination. Finally, the protective efficacy of the vaccine was evaluated after challenge with virulent P. aeruginosa . Following vaccination, the serum antibody levels were significantly higher in mice vaccinated with the EP-rOprH vaccine than in those vaccinated with the rOprH vaccine ( P <0.05). Moreover, the serum antibody levels detected in the EP-rOprH vaccine group were similar to those detected in the OMP vaccine group when P. aeruginosa suspension was used as the coating antigen. However, the levels in the EP-rOprH vaccine group were higher than those in the OMP vaccine and inactivated vaccine groups when the purified rOprH protein was used as the coating antigen ( P <0.05). The level of IFN-γ, IL-2, and IL-4 in mice vaccinated with the EP-rOprH vaccine was significantly higher than that in mice vaccinated with the rOprH vaccine ( P <0.05) and comparable to that in mice vaccinated with the OMP vaccine. The protective rates were 65%, 80%, 80%, and 95% with the rOprH, EP-rOprH, OMP, and inactivated vaccines, respectively. Thus, the extracellular polysaccharide of L. plantarum significantly enhanced the immune response and protection provided by the recombinant subunit vaccine of oprH .

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confidence: 99%

Extracellular polysaccharide of Lactobacillus plantarum enhance immune efficacy of oprH gene recombinant subunit vaccine from Pseudomonas aeruginosa

TIAN,

GONG,

ZHU

et al. 2023

The Journal of Veterinary Medical Science

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Doxycycline combined manure microbes to enhances biofilm formation of the soil plastisphere and increases the surface bio-risk of microplastics vehicle

Liu

Deng

Niu

et al. 2023

Chemical Engineering Journal

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Expression of cupA gene cluster responsible for biofilm formation in Pseudomonas aeruginosa is enhanced against subinhibitory concentration of carbapenems

Cited by 2 publications

References 17 publications

Extracellular polysaccharide of <i>Lactobacillus plantarum</i> enhance immune efficacy of <i>oprH</i> gene recombinant subunit vaccine from <i>Pseudomonas aeruginosa</i>

Extracellular polysaccharide of <i>Lactobacillus plantarum</i> enhance immune efficacy of <i>oprH</i> gene recombinant subunit vaccine from <i>Pseudomonas aeruginosa</i>

Doxycycline combined manure microbes to enhances biofilm formation of the soil plastisphere and increases the surface bio-risk of microplastics vehicle

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