Doxycycline combined manure microbes to enhances biofilm formation of the soil plastisphere and increases the surface bio-risk of microplastics vehicle

Biosafety assessment of laying hens fed different treatments of black soldier flies (Hermetia illucens) under doxycycline stress

“…The most abundant gene in the intestinal contents of the BSF was tetX , with an average abundance of 8.49 × 10 9 copies/g. Compared to the decrease in the highest abundance of ermB in feces, this suggested that the biotransformation of the BSF does not reduce the tetX gene, which is consistent with our previous findings ( Liu et al, 454AD ). The total abundance analysis of ARGs/MGEs in the different groups revealed that group B had the highest abundance, followed by group D, while the levels of ARGs/MGEs in the powder treatment group were lower, which was similar to the trend of antibiotic concentration and further verified the correlation between antibiotics and ARGs/MGEs (Figure S2a).…”

“…Doxycycline concentrations were determined according to previous studies ( Liu et al, 454AD ). Briefly, the sample (the manure or intestinal contents or cotton swab sample weighing 0.5 g) was placed into a 15 mL centrifuge tube, and 5 mL of 0.1 mol/L Na2EDTA-Mcllvaine-methanol was added to the tube.…”