Expression of cofilin 1 is positively correlated with the differentiation of human epithelial ovarian cancer

Zhou, Jianwei; Wang, Yinfen; Jing, Fei; Zhang, Weijiang

doi:10.3892/ol.2012.897

Cited by 32 publications

(29 citation statements)

References 15 publications

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“…In this study, it indicated that the decreased level of DJ-1 by DADS may lead to differentiation and suppressing growth and invasion in HL-60 cells. It displayed that the increased level of cofilin 1 expression was definitely related to the progress of human ovarian cancer differentiation, which demonstrates that the activation of cofilin 1 may accelerate the proliferation and invasion of cancer cells, resulting in the development of ovarian cancer (22). Increase level of cofilin expression induced by diazinon enhanced depolymerization of actin filaments, and then promoted differentiation in neuroblastoma cell line N2a (23).…”

Section: Discussionmentioning

confidence: 91%

Identification of potential targets for differentiation in human leukemia cells induced by diallyl disulfide

Ling

Tan

et al. 2017

International Journal of Oncology

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Diallyl disulfide (DADS) is a primary component of garlic, which has chemopreventive potential. We previously found that moderate doses (15-120 µM) of DADS induced apoptosis and G2/M phase cell cycle arrest. In this study, we observed the effect of low doses (8 µM) of DADS on human leukemia HL-60 cells. We found that DADS could inhibit proliferation, migration and invasion in HL-60 cells, and arrested cells at G0/G1 stage. Then, cell differentiation was displayed by morphologic observation, NBT reduction activity and CD11b evaluation of cytometric flow. It showed that DADS induced differentiation, reduced the ability of NBT and increased CD11b expression. Likewise, DADS inhibited xenograft tumor growth and induced differentiation in vivo. In order to make sure how DADS induced differentiation, we compared the protein expression profile of DADS-treated cells with that of untreated control. Using high resolution mass spectrometry, we identified 18 differentially expressed proteins after treatment with DADS, including four upregulated and 14 downregulated proteins. RT-PCR and western blot assay showed that DJ-1, cofilin 1, RhoGDP dissociation inhibitor 2 (RhoGDI2), Calreticulin (CTR) and PCNA were decreased by DADS. These data suggest that the effects of DADS on leukemia may be due to multiple targets for intervention.

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Section: Discussionmentioning

confidence: 91%

Identification of potential targets for differentiation in human leukemia cells induced by diallyl disulfide

Ling

Tan

et al. 2017

International Journal of Oncology

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“…Upregulation of signaling pathways that inactivate CFN1 has been shown to promote ovarian cancer cell migration, and accordingly, expression of CFN1 positively correlates with differentiation status and progression-free survival of patients in ovarian cancer. 52 We noticed that limiting global proteomic analyses on serous tumor subset led to exclusion of PFN1, 14-3-3, HYOU 1, PP2A1, CAPG and SPTBN1 from the differentially expressed protein hits in the cluster diagram. We asked whether at least some of these proteins failed to meet the criterion for statistical significance because of non-parametric Wilcoxon Rank test adopted in the global proteomic analyses and even smaller sample size in serous cohort analyses.…”

Section: Resultsmentioning

confidence: 98%

BRCA1 deficiency in ovarian cancer is associated with alteration in expression of several key regulators of cell motility – A proteomics study

et al. 2015

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Functional loss of expression of breast cancer susceptibility gene 1(BRCA1) has been implicated in genomic instability and cancer progression. There is emerging evidence that BRCA1 gene product (BRCA1) also plays a role in cancer cell migration. We performed a quantitative proteomics study of EOC patient tumor tissues and identified changes in expression of several key regulators of actin cytoskeleton/cell adhesion and cell migration (CAPN1, 14-3-3, CAPG, PFN1, SPTBN1, CFN1) associated with loss of BRCA1 function. Gene expression analyses demonstrate that several of these proteomic hits are differentially expressed between early and advanced stage EOC thus suggesting clinical relevance of these proteins to disease progression. By immunohistochemistry of ovarian tumors with BRCA1(+/+) and BRCA1(null) status, we further verified our proteomic-based finding of elevated PFN1 expression associated with BRCA1 deficiency. Finally, we established a causal link between PFN1 and BRCA1-induced changes in cell migration thus uncovering a novel mechanistic basis for BRCA1-dependent regulation of ovarian cancer cell migration. Overall, findings of this study open up multiple avenues by which BRCA1 can potentially regulate migration and metastatic phenotype of EOC cells.

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“…CFL1, the actin regulatory protein, is important in tumor growth and progression (45). It has been reported that CFL1 is involved in tumor progression in ovarian carcinoma, almost 64% of all ovarian tumors are positive for CFL1 (46). In prostate cancer, knockdown of CFL1 was reported to increase sensitivity to docetaxel, a chemotherapeutic agent (47).…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of selective cytotoxic anticancer properties of flavonoids isolated from Citrus platymamma on A549 human lung cancer cells

Nagappan

Hong

Lee

et al. 2016

Molecular Medicine Reports

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Citrus platymamma Hort. ex Tanaka (Byungkyul in Korean) has been used in Korean folk medicine for the treatment of inflammatory disorders and cancer. However, the molecular mechanism underlying the anticancer properties of flavonoids isolated from C. platymamma (FCP) remains to be elucidated. Therefore, the present study attempted to identify the key proteins, which may be important in the anticancer effects of FCP on A549 cells using a proteomic approach. FCP showed a potent cytotoxic effect on the A549 human lung cancer cells, however, it had no effect on WI‑38 human fetal lung fibroblasts at the same concentrations. Furthermore, 15 differentially expressed protein spots (spot intensities ≥2‑fold change; P<0.05) were obtained from comparative proteome analysis of two‑dimensional gel electrophoresis maps of the control (untreated) and FCP‑treated A549 cells. Finally, eight differentially expressed proteins, one of which was upregulated and seven of which were downregulated, were successfully identified using matrix‑assisted laser desorption/ionization time‑of‑flight/time‑of‑flight tandem mass spectrometry and peptide mass fingerprinting analysis. Specifically, proteins involved in signal transduction were significantly downregulated, including annexin A1 (ANXA1) and ANXA4, whereas 14‑3‑3ε was upregulated. Cytoskeletal proteins, including cofilin‑1 (CFL1), cytokeratin 8 (KRT8) and KRT79, and molecular chaperones/heat shock proteins, including endoplasmin, were downregulated. Proteins involved in protein metabolism, namely elongation factor Ts were also downregulated. Consistent with results of the proteome analysis, the immunoblotting results showed that 14‑3‑3ε was upregulated, whereas CFL1, ANXA4 and KRT8 were downregulated in the FCP‑treated A549 cells. The majority of the proteins were involved in tumor growth, cell cycle, apoptosis, migration and signal transduction. These findings provide novel insights into the molecular mechanisms underlying FCP-induced anticancer effects on A549 cells.

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Expression of cofilin 1 is positively correlated with the differentiation of human epithelial ovarian cancer

Cited by 32 publications

References 15 publications

Identification of potential targets for differentiation in human leukemia cells induced by diallyl disulfide

Identification of potential targets for differentiation in human leukemia cells induced by diallyl disulfide

BRCA1 deficiency in ovarian cancer is associated with alteration in expression of several key regulators of cell motility – A proteomics study

Proteomic analysis of selective cytotoxic anticancer properties of flavonoids isolated from Citrus platymamma on A549 human lung cancer cells

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