Expression of ClPAP and ClPSY1 in watermelon correlates with chromoplast differentiation, carotenoid accumulation, and flesh color formation

Fang, Xufeng; Liu, Shi; Gao, Peng; Liu, Hongyu; Wang, Xuezheng; Luan, Feishi; Zhang, Qian; Dai, Zhigang

doi:10.1016/j.scienta.2020.109437

Cited by 32 publications

(42 citation statements)

References 40 publications

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“…In this study, the esh color was observed all white at the early fruit developmental stages and then changed to vivid color at the later stages, among the ve genotypes. Lycopene was the main pigment in red and pink esh genotypes, these results were consistent with the previous report [34,40]. The orange esh color is largely determined by the content of β-carotene, the same result as the previous report [10], moreover, the orange esh has a higher content of violaxanthin zeaxanthin, neoxanthin, lutein, and β-cryptoxanthin than other genotypes.…”

Section: Discussionsupporting

confidence: 92%

“…The expression level of Cla014416 signi cantly higher in yellow and orange esh color materials in this study (Additional le 13: Fig. S3g, Additional le 14 and 21: Table S7 and S9), different from the previous report that ClPAP highly expressed in red and orange color lines [34]. Cla000655 encodes a cytochrome P450, is related to the protein lutein de cient 5 (CYP97A3) in Arabidopsis thaliana [35] and highly expressed in yellow and orange color fruits (Additional le 13: Fig.…”

Section: Clustering Of Degs Into Six Groups Based On Gene Expression contrasting

confidence: 99%

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Transcriptome Regulation of Carotenoids in Five Flesh-Colored Watermelon (Citrullus lanatus)

Yuan

Umer

et al. 2020

Preprint

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Background: Fruit flesh color in watermelon (Citrullus lanatus) is a great index for evaluation of the appearance quality and a key contributor influencing consumers preferences, but the molecular mechanisms of this intricate trait remain largely unknown. Here, the carotenoids and transcriptome dynamics during fruit development in watermelon cultivars with 5 different flesh colors were analyzed.Results: A total of 13 carotenoids and 16,781 differentially expressed genes (DEGs) including 1,295 transcription factors (TFs) were detected during the development of five watermelon genotypes. A number of structural genes and transcription factors were found to be involved in the carotenoid biosynthesis pathway using comparative transcriptome analysis. Furthermore, we performed weighted gene co-expression network analysis and predicted hub genes in 6 main modules determining carotenoids contents. Cla018406 (a Chaperone protein dnaJ-like protein) maybe a candidate gene for β-carotene and highly expressed in orange flesh colored fruit. Cla007686 (a zinc finger CCCH domain-containing protein) was highly expressed in the red color watermelon, maybe a key regulator for lycopene accumulation. Cla003760 (merbrane protein) and Cla007686 (photosystenI reaction center subunit II) are predicted to be hub genes and play an important role in yellow flesh color formation.Conclusions: These results provide an important resource for dissecting the molecular basis and candidate genes governing flesh color formation in watermelon fruit.

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Section: Discussionsupporting

confidence: 92%

Section: Clustering Of Degs Into Six Groups Based On Gene Expression contrasting

confidence: 99%

Transcriptome Regulation of Carotenoids in Five Flesh-Colored Watermelon (Citrullus lanatus)

Yuan

Umer

et al. 2020

Preprint

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“…S2g, Additional le 1: Table S7 and S9). This result is different from the previous report that ClPAP highly expressed in red/orange than yellow /white color genotypes [44], possibly due to different genotypes used in these two studies. Cla000655 (encodes a cytochrome P450) is homologous to the protein lutein de cient 5 (CYP97A3), which involved in the biosynthesis of xanthophylls in Arabidopsis thaliana [45] (E-value: 6.5e-267, Identity: 80.47%).…”

Section: Co-expression Network Analysis Identi Ed Carotenoid-related contrasting

confidence: 99%

Transcriptome regulation of carotenoids in five flesh-colored watermelon (Citrullus lanatus)

Yuan

Umer

et al. 2021

Preprint

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Background: Fruit flesh color in watermelon (Citrullus lanatus) is a great index for evaluation of the appearance quality and a key contributor influencing consumers' preferences, but the molecular mechanism of this intricate trait remain largely unknown. Here, the carotenoids and transcriptome dynamics during the fruit development of cultivated watermelon with five different flesh colors were analyzed.Results: A total of 13 carotenoids and 16781 differentially expressed genes (DEGs) including 1295 transcription factors (TFs) were detected in five watermelon genotypes during the fruit development. The comprehensive accumulation patterns of carotenoids were closely related to flesh color. A number of potential structural genes and transcription factors were found to be associated with the carotenoid biosynthesis pathway using comparative transcriptome analysis. The differentially expressed genes were divided into six subclusters and distributed in different GO terms and metabolic pathways. Furthermore, we performed weighted gene co-expression network analysis and predicted hub genes in six main modules determining carotenoid contents. Cla018406 (a chaperone protein dnaJ-like protein) may be a candidate gene for β-carotene accumulation and highly expressed in orange flesh-colored fruit. Cla007686 (a zinc finger CCCH domain-containing protein) was highly expressed in the red flesh-colored watermelon, maybe a key regulator of lycopene accumulation. Cla003760 (membrane protein) and Cla021635 (photosystem I reaction center subunit II) were predicted to be hub genes and may play an essential role in yellow flesh formation.Conclusions: The composition and contents of carotenoid in five watermelon genotypes vary greatly. A series of candidate genes were revealed through combined analysis of metabolites and transcriptome. These results provide an important data resource for dissecting the candidate genes and molecular basis governing flesh color formation in watermelon fruit.

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“…Cheung et al, (1993) reported that a substantial amount of chlorophyll was retained in the gf mutant tomato fruit due to the presence of chloroplast thylakoid grana in the plastids of mature fruits. Pan et al (2013) (Cla008831 in watermelon referenced genome 97103 v1) also had an expression pattern similarly to ClPSY1, which might be caused by chloroplasts transforming into chromoplasts as the fruit ripens and carotenoid accumulation in plastid globules (Fang et al, 2020).…”

Section: Discussionmentioning

confidence: 98%

“…Simkin et al (2007) overexpressed the pepper fibrillin gene in tomato, leading to a doubling of lycopene content in the fruit. Fang et al (2020) found associations of the expression of plastid lipidassociated genes (ClPAP) and phytoene synthase genes (ClPSY1) with chromoplast differentiation, carotenoid accumulation, and flesh colour formation in watermelon. The ClPSY gene is responsible for carotenoid biosynthesis, showing the same expression pattern as ClPAP (Cla014416) in watermelon.…”

Section: Introductionmentioning

confidence: 99%

Quantitative trait loci and candidate genes responsible for pale green flesh colour in watermelon (Citrullus lanatus)

Pei

Wang

et al. 2021

Plant Breeding

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Watermelon (Citrullus lanatus) is a fleshy, sweet fruit that has become one of the most popular horticultural crops worldwide. Fruit flesh colour is an important commercial trait that guides consumer choice and watermelon breeder selection. Flesh colour is determined by different compositions of chlorophyll and carotenoids (Zhang et al., 2016). Variations in watermelon flesh colour have been designated as scarlet red, red, pink, orange, canary yellow, pale yellow, and white based on carotenoid composition. Red flesh (including scarlet red and pink) watermelons mainly consist of lycopene (Grassi

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Expression of ClPAP and ClPSY1 in watermelon correlates with chromoplast differentiation, carotenoid accumulation, and flesh color formation

Cited by 32 publications

References 40 publications

Transcriptome Regulation of Carotenoids in Five Flesh-Colored Watermelon (Citrullus lanatus)

Transcriptome Regulation of Carotenoids in Five Flesh-Colored Watermelon (Citrullus lanatus)

Transcriptome regulation of carotenoids in five flesh-colored watermelon (Citrullus lanatus)

Quantitative trait loci and candidate genes responsible for pale green flesh colour in watermelon (Citrullus lanatus)

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