Quantitative trait loci and candidate genes responsible for pale green flesh colour in watermelon (<i>Citrullus lanatus</i>)

Pei, Shuang; Li, Zheng; Wang, Xuezheng; Luan, Feishi; Dai, Zhigang; Yang, Zhongzhou; Zhang, Qian; Liu, Shi

doi:10.1111/pbr.12908

Cited by 15 publications

(14 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the last decade, whole genome SNP-derived CAPS markers have emerged as potential genetic markers for uncovering the genetic regions affecting complex types of qualitative and quantitative traits in differentially derived populations of unexplored botanical groups. For example, powdery mildew resistance (Li et al, 2017 ), stripped fruit skin (Liu et al, 2019 ), ovary-fruit associated morphology (Amanullah et al, 2018 , 2020 , 2021 ), plant height (Zhang et al, 2021 ), pale green and dark red color flesh (Wang et al, 2019 ; Pei et al, 2021 ), egusi seed traits (Osae et al, 2021 ), and fruit peel hardness (Yang et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Development of Whole Genome SNP-CAPS Markers and Preliminary QTL Mapping of Fruit Pedicel Traits in Watermelon

et al. 2022

Self Cite

View full text Add to dashboard Cite

Fruit pedicel (FP) is an important determinant of premium fruit quality that directly affects commercial market value. However, in-depth molecular and genetic basis of pedicel-related traits has not been identified in watermelon. Herein, a quantitative trait locus (QTL) mapping strategy was used to identify the potential genetic regions controlling FP traits based on newly derived whole-genome single nucleotide polymorphism based cleaved amplified polymorphism sequence (SNP-CAPS) markers. Next-generation sequencing based whole-genome re-sequencing of two watermelon parent lines revealed 98.30 and 98.40% of average coverage, 4,989,869 SNP variants, and 182,949 CAPS loci pairs across the reference genome, respectively. A total of 221 sets of codominant markers exhibited 46.42% polymorphism rate and were effectively genotyped within 100-F2:3 derived mapping population. The developed linkage map covered a total of 2,630.49 cM genetic length with averaged 11.90 cM, and depicted a valid marker-trait association. In total, 6 QTLs (qFPL4.1, qFPW4.1, qFPD2.1, qFPD2.2, qFPD8.1, qFPD10.1) were mapped with five major effects and one minor effect between the whole genome adjacent markers positioned over distinct chromosomes (02, 04, 08, 10), based on the ICIM-ADD mapping approach. These significant QTLs were similarly mapped in delimited flanking regions of 675.10, 751.38, 859.24, 948.39, and 947.51 kb, which collectively explained 8.64–13.60% PVE, respectively. A highly significant and positive correlation was found among the observed variables. To our knowledge, we first time reported the mapped QTLs/genes affecting FP traits of watermelon, and our illustrated outcomes will deliver the potential insights for fine genetic mapping as well as functional gene analysis through MAS-based breeding approaches.

show abstract

Section: Introductionmentioning

confidence: 99%

Development of Whole Genome SNP-CAPS Markers and Preliminary QTL Mapping of Fruit Pedicel Traits in Watermelon

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The MELO3C011657 encodes the tetrapyrrole-binding protein, which has been significantly demonstrated in the Arabidopsis papp5 mutant, and it has been shown that the tetrapyrrole-binding protein is mainly associated with PAPP5 in complex components involved in the downstream of the plastid signaling pathway of tetrapyrrole Mg-ProtoIX/Mg-ProtoIX-ME [ 58 ]. The complexity of these components can act as a negative regulator of PhANG expression during chloroplast development, which ultimately affects the chloroplast morphology associated with plant flower color morphology [ 59 ]. Most importantly, we believe that our current research findings demonstrate comprehensive bio-information about the candidate pathways and associated genes involved in the determination of melon stigma color.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis Identified Key Pathways and Genes Regulating Differentiated Stigma Color in Melon (Cucumis melo L.)

Amanullah

Shi

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Stigma color is an important morphological trait in many flowering plants. Visual observations in different field experiments have shown that a green stigma in melons is more attractive to natural pollinators than a yellow one. In the current study, we evaluated the characterization of two contrasted melon lines (MR-1 with a green stigma and M4-7 with a yellow stigma). Endogenous quantification showed that the chlorophyll and carotenoid content in the MR-1 stigmas was higher compared to the M4-7 stigmas. The primary differences in the chloroplast ultrastructure at different developmental stages depicted that the stigmas of both melon lines were mainly enriched with granum, plastoglobulus, and starch grains. Further, comparative transcriptomic analysis was performed to identify the candidate pathways and genes regulating melon stigma color during key developmental stages (S1–S3). The obtained results indicated similar biological processes involved in the three stages, but major differences were observed in light reactions and chloroplast pathways. The weighted gene co-expression network analysis (WGCNA) of differentially expressed genes (DEGs) uncovered a “black” network module (655 out of 5302 genes), mainly corresponding to light reactions, light harvesting, the chlorophyll metabolic process, and the chlorophyll biosynthetic process, and exhibited a significant contribution to stigma color. Overall, the expression of five key genes of the chlorophyll synthesis pathway—CAO (MELO03C010624), CHLH (MELO03C007233), CRD (MELO03C026802), HEMA (MELO03C011113), POR (MELO03C016714)—were checked at different stages of stigma development in both melon lines using quantitative real time polymerase chain reaction (qRT-PCR). The results exhibited that the expression of these genes gradually increased during the stigma development of the MR-1 line but decreased in the M4-7 line at S2. In addition, the expression trends in different stages were the same as RNA-seq, indicating data accuracy. To sum up, our research reveals an in-depth molecular mechanism of stigma coloration and suggests that chlorophyll and related biological activity play an important role in differentiating melon stigma color.

show abstract

“…A SNP marker linked to the lycopene b-cyclase gene ( LCYB ) has been developed for genotype selection ( Bang et al, 2007 ). Kompetitive allele-specific polymorphism markers co-segregating with pale flesh color phenotype have been developed for genetic analysis of watermelon ( Pei et al, 2021 ). The KASP marker ( chr06_7040350 ) effectively distinguished white and pale-yellow fleshed watermelons useful for marker-assisted breeding ( Wang et al, 2017 ).…”

Section: Molecular Markers and Quantitative Trait Loci Analysis Of Ph...mentioning

confidence: 99%

“…Also, the β-carotene hydroxylase CHYB gene (gene ID: Cla011420 ) was mapped on chromosome 1 ( Jin et al, 2019 ). The QTL qfc10.1 located on chromosome 10 provides the pale flesh color of watermelon ( Pei et al, 2021 ). Carotenoid biosynthesis enzymes carotenoid isomerase gene ( CRTISO ; gene ID: Cla017593 ) is located on chromosome 10 ( Jin et al, 2019 ).…”

Section: Molecular Markers and Quantitative Trait Loci Analysis Of Ph...mentioning

confidence: 99%

Genetic Analysis of Fruit Quality Traits in Sweet Watermelon (Citrullus lanatus var. lanatus): A Review

et al. 2022

View full text Add to dashboard Cite

show abstract

Quantitative trait loci and candidate genes responsible for pale green flesh colour in watermelon (Citrullus lanatus)

Cited by 15 publications

References 41 publications

Development of Whole Genome SNP-CAPS Markers and Preliminary QTL Mapping of Fruit Pedicel Traits in Watermelon

Development of Whole Genome SNP-CAPS Markers and Preliminary QTL Mapping of Fruit Pedicel Traits in Watermelon

Comparative Transcriptome Analysis Identified Key Pathways and Genes Regulating Differentiated Stigma Color in Melon (Cucumis melo L.)

Genetic Analysis of Fruit Quality Traits in Sweet Watermelon (Citrullus lanatus var. lanatus): A Review

Contact Info

Product

Resources

About