Expression of Claudin-1, -2, -3, -4, -5 and -7 Proteins in Benign and Malignant Canine Mammary Gland Epithelial Tumours

Jakab, Cs.; Halász, Júlia; Szász, Attila Marcell; Kiss, András; Schaff, Z.; Rusvai, Miklós; Gálfi, P.; Kulka, Janina

doi:10.1016/j.jcpa.2008.08.001

Cited by 21 publications

(22 citation statements)

References 38 publications

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“…Claudin-4 does not seem to be able to help in distinguishing hyperplasias from adenomas. In our previous study we have described a similar strong expression of the claudin-4 molecule in lobular hyperplasia and simple adenoma of the canine mammary gland (Jakab et al, 2008c). Perianal epitheliomas are rare neoplastic lesions with low-grade malignancy.…”

Section: Discussionmentioning

confidence: 77%

“…In the present study, we have detected claudin-4 overexpression in poorly differentiated carcinomas of canine hepatoid glands. In our previous studies we have described claudin-4 overexpression in grade I, II and III infiltrating simple carcinomas of the canine mammary gland (Jakab et al, 2008c) and in the lung metastases of solid (grade III) canine mammary gland carcinomas (Jakab et al, 2009). Metastasis is common in poorly differentiated hepatoid cell carcinomas (Goldschmidt and Hendrick, 2000).…”

Section: Discussionmentioning

confidence: 96%

“…We have reported that claudin-4 is one of the integral constituents of tight junction structures of the normal canine mammary gland (Jakab et al, 2008a) as well as of primary (Jakab et al, 2008c) and secondary epithelial tumours of the canine mammary gland (Jakab et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

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Expression of the claudin-4 molecule in benign and malignant canine hepatoid gland tumours

Jakab

Rusvai

Szabó

et al. 2009

Acta Veterinaria Hungarica

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Claudins are integral membrane proteins of the tight junction structures expressed by epithelial and endothelial cells. The present study has evaluated the expression of claudin-4 in 10 normal canine hepatoid glands and in 67 hepatoid glands with hyperplastic and neoplastic lesions. The lesions studied included normal hepatoid glands (n = 10), nodular hyperplasias (n = 10), adenomas (n = 12), epitheliomas (n = 15), differentiated carcinomas (n = 15) and anaplastic carcinomas (n = 15). There was an intensive expression of claudin-4 in normal canine hepatoid glands as well as in hyperplasias and adenomas. Claudin-4 was detected as a well-localised linear circumferential membranous staining pattern of epithelial cells (mature hepatoid cells) in normal hepatoid glands, perianal gland hyperplasias and adenomas. In nodular hyperplasia and adenoma, the reserve cells showed membrane positivity for the claudin-4 molecule. There was a weaker expression in hepatoid gland epitheliomas. In the epitheliomas, the basaloid reserve cells never expressed the claudin-4 molecule. The multiple small parts of epitheliomas in which the cells exhibited typical hepatoid features showed a welllocalised linear circumferential membranous staining pattern for claudin-4. The numerical score for cellular expression of claudin-4 was higher in differentiated carcinomas than in epitheliomas, but moderately lower than in adenomas. The anaplastic, poorly differentiated hepatoid gland carcinomas showed an overexpression of claudin-4. These results suggest that low claudin-4 expression in epitheliomas is a molecular characteristic indicative of increasing cellular disorientation, detachment motility and invasion by tumour cells, and claudin-4 seems to be helpful in distinguishing undifferentiated carcinomas from differentiated carci-

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 96%

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Expression of the claudin-4 molecule in benign and malignant canine hepatoid gland tumours

Jakab

Rusvai

Szabó

et al. 2009

Acta Veterinaria Hungarica

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“…The expression of claudin-1, -2, -3, -4, -5 and -7 was investigated in the normal canine mammary gland and in hyperplastic and neoplastic mammary lesions (Jakab et al, 2008). The results suggest that loss or reduction of expression of claudin-1, -2, -5 and -7 may lead to cellular disorientation, detachment and invasion in canine mammary neoplasia.…”

mentioning

confidence: 98%

“…Although no data have yet been published in the veterinary literature on the occurrence of claudin proteins in the healthy canine gastric mucosa, the cross-reaction of humanised anti-claudin antibodies with the different canine tissues has been proved in several studies (Yu et al, 2003;Jakab et al, 2008Jakab et al, , 2010Sas et al, 2008).…”

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confidence: 99%

Expression of claudins in the normal canine gastric mucosa

Psáder

Jakab

Máthé

et al. 2014

Acta Veterinaria Hungarica

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The aim of the present study was to investigate the expression pattern of claudin-1, -2, -3, -4, -5, -6, -7, -8, -10 and -18 in the intact fundic and pyloric gastric mucosa of dogs. Intense, linear, membranous claudin-18 positivity was detected in the surface gastric cells and in the epithelial cells of the gastric glands both in the fundic and pyloric stomach regions. The mucous neck cells in the apical part of the glands, furthermore the parietal cells and chief cells of the basal part of the gland were all positive for claudin-18, in the same way as the enteroendocrine cells. Cells of the basal part of the pyloric glands showed intense, linear, membranous claudin-2 positivity, but cells of the superficial portion of these glands and the surface gastric cells in this region were claudin-2 negative. Fibroblasts, endothelial cells, lymphocytes of the propria layer, smooth muscle cells and vegetative neurons were all negative for claudin-2. All gastric epithelial cells were negative for claudin-1, -3, -4, -5, -6, -7, -8 and -10. The endothelial cells of the propria layer had intense claudin-5 positivity. We assume that claudin-18 forms a paracellular barrier against gastric acid in the healthy canine stomach, in the same way as in mice.

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Altered expression of tight junction proteins in mammary epithelium after discontinued suckling in mice

Markov

Круглова

Fomina

et al. 2011

Pflugers Arch - Eur J Physiol

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Milk production is modulated by the paracellular barrier function of tight junction (TJ) proteins located in the mammary epithelium. The aim of our study was the molecular analysis of TJs in native lactating murine mammary gland epithelium as this process may strongly challenge epithelial barrier properties and regulation. Mammary gland tissue specimens from lactating control mice and animals after a 20-h interruption of suckling were prepared; histological analyses were performed by light and electron microscopy; and expression of TJ proteins was detected by PCR, Western blotting, immunofluorescent staining, and confocal laser scanning microscopy. Discontinuation of suckling resulted in a substantial accumulation of milk in mammary glands, an increase of alveolar size, and a flattening of epithelial cells without effects on inflammatory indicators. In control tissues, PCR and Western blots showed signals for occludin, and claudin-1, -2, -3, -4, -5, -7, -8, -15, and -16. After a 20-h accumulation of milk, expression of two sealing TJ proteins, claudin-1 and -3, was markedly increased, whereas two TJ proteins involved in cation transport, claudin-2 and -16, were reduced. Real-time PCR validated increased transcripts of claudin-1 and claudin-3. During extension of mammary glands in the process of lactation, claudin-1 and -3 are markedly induced and claudin-2 and -16 are decreased. Volume and composition of milk might be strongly dependent on this counter-regulation of sealing claudins with permeability-mediating claudins, indicating a physiological process of a tightening of TJs against a back-leak of solutes and ions from the alveolar lumen.

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Expression of Claudin-1, -2, -3, -4, -5 and -7 Proteins in Benign and Malignant Canine Mammary Gland Epithelial Tumours

Cited by 21 publications

References 38 publications

Expression of the claudin-4 molecule in benign and malignant canine hepatoid gland tumours

Expression of the claudin-4 molecule in benign and malignant canine hepatoid gland tumours

Expression of claudins in the normal canine gastric mucosa

Altered expression of tight junction proteins in mammary epithelium after discontinued suckling in mice

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