Expression of cell surface receptors and oxidative metabolism modulation in the clinical continuum of sepsis

Abstract

Background Infection control depends on adequate microbe recognition and cell activation, yet inflammatory response may lead to organ dysfunction in sepsis. The aims of this study were to evaluate cell activation in the context of sepsis and its correlation with organ dysfunction.

Lipopolysaccharide-induced expression of cell surface receptors and cell activation of neutrophils and monocytes in whole human blood

“…In agreement with numerous other studies we show that not only monocytes, but also neutrophils, express the signaling receptor for LPS, TLR4 (11). However, in contrast to some reports (18,19), we observed no down-regulation of TLR4 on monocytes during the initial period of stimulation (up to 3 h after LPS), but rather a weak, transient increase at 6 h after LPS.…”

“…LPS-induced cell activation in whole blood has the advantage of preserving the microenvironment of LPS and cell interaction as it occurs in vivo; however, studies of the effects of LPS on different cell populations in whole blood are scarce. Flow cytometry permits the study of the effects of LPS in whole blood at the cellular level (10,11). Macrophages are considered to be the primary targets of LPS, and neutrophils have also been reported as highly LPS-responsive cells.…”

Evaluation of TLR4 expression and chosen parameters of oxidative-antioxidative balance in young children with food allergy.

“…ROS created in the cell have influence on different structures and, can also activate NF-κB and numerous genes. ROS excess is neutralized by antioxidative system [20][21][22]. Physiologically, the balance between oxidative and antioxidative processes in the body is maintained.…”

Augmented Passive Immunotherapy with P4 Peptide Improves Phagocyte Activity in Severe Sepsis

“…Neutrophils with increased expression of CD66b are associated with an up-regulated respiratory burst in samples isolated from patients with severe sepsis (24). Baseline CD66b was higher in early phase compared to latent phase samples ( Figure 3A) and this was associated with greater neutrophil-bead association ( Figure 3B).…”