Expression of cell cycle proteins according to HPV status in oral squamous cell carcinoma affecting young patients: a pilot study

Abstract

No significant difference exists in the expression of cell cycle proteins studied between HR-HPV DNA-positive and HR-HPV DNA-negative OSCC affecting young patients.

Evaluation of HPV and EBV in OSCC and the expression of p53, p16, E‐cadherin, COX‐2, MYC, and MLH1

“…In addition, it was verified that amplification of c‐myc, assessed by real‐time PCR, was significantly associated with levels of its protein product. In another study, considering only nuclear staining, there was no statistical difference in the MYC expression between HR‐HPV DNA‐positive and ‐negative groups (Miranda Galvis et al., 2018). In the study by Popović et al.…”

“…Other authors found no association between p53 expression and HPV presence as well, in which the viral detection was performed by PCR (Angiero et al., 2010; Emmett et al., 2017; Greer et al., 2008; Miranda Galvis et al., 2018; Nemes et al., 2006; Oliveira et al., 2009; Shindoh et al., 1995). Nonetheless, as in our study, Greer et al.…”

“…HPV has been investigated in oral cavity carcinomas along the last three decades; however, only few studies have correlated it with tumor markers. In turn, the main immunohistochemical markers already analyzed include the following: p16 INK4a , p21 WAF1 , p53, pRb, Ki67, PCNA, bcl‐2, Oncostatin M, EGFR, β‐catenin, MYC, c‐Jun, Cyclin D1, and C‐ErbB2 (Angiero et al., 2010; Chuerduangphui et al., 2016, 2017; Duray et al., 2012; Emmett et al., 2017; Greer et al., 2008; Ishibashi et al., 2011; Miranda Galvis et al., 2018; Nemes et al., 2006; Nwanze et al., 2015; Oliveira et al., 2009; Rodríguez‐Santamarta et al., 2016; Singh et al., 2017). Nevertheless, until the elaboration of this manuscript, we realize a lack of studies addressing HPV infection and the expression of MLH1, COX‐2, E‐cadherin, and MYC in OSCC, a set of human proteins that appears to have an intricate relationship with the HPV oncoproteins E6 and E7, and some EBV latent components, in the developing of oral carcinomas, making up a tumor signaling pathway as proposed in a recent review (de Lima, Teodoro, da Silva, et al., 2019).…”

“…On the contrary, other studies have not observed association between p16 expression and HPV infection; in some of them, the HPV detection was performed by ISH (Satgunaseelan et al., 2016), others by PCR‐based methods (Emmett et al., 2017; Ghantous et al., 2018; Götz et al., 2016; Greer et al., 2008; Ishibashi et al., 2011; Miranda Galvis et al., 2018; Nemes et al., 2006; Palve et al., 2018; Singh et al., ,2015, 2017; Sundberg et al., 2019; Yang et al., 2019) or using both techniques (Belobrov et al., 2018; Rodríguez‐Santamarta et al., 2016). Ishibashi et al.…”

Human papillomavirus DNA and p16 expression in head and neck squamous cell carcinoma in young French patients

“…We conducted a retrospective study including 39 patients aged <45 years and a matched group of 38 patients aged ≥45 years with HNSCC treated at our University Hospital over a period of 13 years. There is currently no consensus in the literature regarding the threshold for differentiating between young and old patients, with previous authors using either 40 or 45 years; 6 , 20 – 22 We therefore chose 45 years as the age threshold to define young and old patients.…”