Expression of CD16, CD18 and CD56 in tributyltin-exposed human natural killer cells

Tributyltin exposure causes decreased granzyme B and perforin levels in human natural killer cells

“…decreased cytotoxic function. In previous studies we have shown that a 24 h exposure to 200 nM TBT also caused an approximately 40% decrease in the ability of NK cells to bind to the K562 targets cells (Whalen et al, 2002). This same study also showed decreases of both CD16 and CD56 expression after a 24 h exposure to TBT.…”

“…In those studies 200 nM had no effect on NK function after a 1 h exposure and so 300 nM is used for 1 h exposures. A 1 h exposure to 300 nM TBT (followed by 24 or 48 h in TBT-free media) produced persistent inhibition of NK cytotoxic function in a previous study (Whalen et al, 2002). Levels of TBT measured in human blood ranged from undetectable to a high of 260 nM (one sample).…”

“…The cell layer was collected and washed twice with PBS and stored in complete media at 1 million cells/mL. The resulting cell preparation was >95% CD16 + , 0% CD3 + by fluorescence microscopy (Whalen et al, 2002).…”

“…This TBT solution was diluted in gelatin media (0.5% gelatin replaced the calf serum in complete medium) to achieve final concentrations. The concentrations of TBT used in treating the cells were 200-300 nM based on previous studies (Whalen et al, 1999(Whalen et al, , 2002. In those studies 200 nM had no effect on NK function after a 1 h exposure and so 300 nM is used for 1 h exposures.…”

Evaluation of the Effects of Tributiltin (TBT) on Chromosomes of the Neotropical Fish Astyanax sp. (Pisces, Tetragonopterinae)

“…TBT, one of the organic compounds of the tin, is used to preservate wood, textile products, leather and as biocide in antifouling paints for both, body hull and in production of PVC and other plastics, being discharged in surface water causing direct environmental impact in aquatic ecosystems (Takahasi et al 1999, Whalen et al 2002. Some studies have evidenced that TBT was associated to damage and decrease of marine life (Alzieu 1991(Alzieu , 2000.…”