Expression of CD117 (c-Kit) on Circulating B Cells in Pediatric Schistosomiasis

Onkanga, Isaac O.; Hamilton, Rachael E.; Mwinzi, Pauline N. M.; Schneider, Thomas; Ondigo, Bartholomew N.; Sang, Huldah C; Ondari, Edna; Rawago, Fredrick; Jaoko, Walter; Odiere, Maurice R.; Ganley-Leal, Lisa

doi:10.1128/iai.00160-22

Cited by 2 publications

(7 citation statements)

References 57 publications

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“…Interestingly, increased CD193+ B cells were associated with lower plasma IgE levels as well as CD27+ memory and IgE+ B cell percentages. Coupled with the data that shows a positive correlation between CD117, a marker of immature B cells, and CD193 expression, these data suggest that CD193 expression is associated with reduction of protective immunity 13 …”

Section: Discussionsupporting

confidence: 55%

“…This observation adds to our recent data explaining the inability of children to develop significant immunity during schistosomiasis infection. 13 These findings reported in this work for the first time, to our knowledge, deserve further characterization to shed more light on their functional significance critical for understanding multiple diseases. Worth noting is that percentages of circulating CD193+ B cells were significantly reduced following praziquantel treatment, which is known to increase immune responses, especially anti-schistosome IgE, towards protective levels in susceptible children.…”

Section: Discussionmentioning

confidence: 69%

“…Coupled with the data that shows a positive correlation between CD117, a marker of immature B cells, and CD193 expression, these data suggest that CD193 expression is associated with reduction of protective immunity. 13 These observations were surprising considering that several previous reports have generally demonstrated that CD193 is primarily expressed by inflammatory cells associated with Th2 immune responses. 28,29,34,47 For example, it has previously been shown that CD193 expression defines a subset of T lymphocytes that respond to eotaxin-1 and co-localize with eosinophils at sites of allergic-like inflammation, such as in the gut.…”

Section: Discussionmentioning

confidence: 95%

“…In this regard, we recently demonstrated that Schistosoma mansoni infection in pre-pubescent children appears to elicit immature B cells from the bone marrow and thus likely affects the ability to develop significant immunity to infection. 13 Class switching to IgE by B cells and ultimate production of IgE by plasma cells is commonly induced in the lymph nodes. [14][15][16][17] B cell trafficking from the bloodstream, or from sites of infection, to the lymph nodes has been shown to be facilitated by the expression of chemokine receptors on the B cell surface.…”

Section: Introductionmentioning

confidence: 99%

“…The mechanisms behind the heterogenous ability to generate and maintain elevated IgE levels remains poorly understood. In this regard, we recently demonstrated that Schistosoma mansoni infection in pre‐pubescent children appears to elicit immature B cells from the bone marrow and thus likely affects the ability to develop significant immunity to infection 13 …”

Section: Introductionmentioning

confidence: 99%

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CD193 (CCR3) expression by B cells correlates with reduced IgE production in paediatric schistosomiasis

Onkanga

Sang

Hamilton³

et al. 2023

Parasite Immunology

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We demonstrate that CD193, the eotaxin receptor, is highly expressed on circulating B cells in paediatric schistosomiasis mansoni. CD193 plays a role in directing granulocytes into sites of allergic-like inflammation in the mucosa, but little is known about its functional significance on human B cells. We sought to characterize CD193 expression and its relationship with S. mansoni infection. We found that CD193+ B cells increased with the intensity of schistosome infection. In addition, a significant negative association was observed between CD193 expression by B cells and IgE production. Decreased IgE levels are generally associated with susceptibility to reinfection. B cell stimulation with eotaxin-1 increased CD193 levels whereas IL-4 led to a reduction. This was supported by plasma levels of eotaxin-1 correlating with CD193 levels on B cells and other cells. In contrast, CD193 expression was induced on naive B cells with a combination of IL-10 and schistosome antigens. Whereas T cells had a modest increase in CD193 expression, only B cell CD193 appeared functionally chemotactic to eotaxin-1. Thus, CD193+ B cells, which co-express CXCR5, may be enroute to sites with allergic-like inflammation, such as gastrointestinal follicles, or even to Th2 granulomas, which develop around parasite eggs. Overall, our results suggest that schistosome infection may promote CD193 expression and suppress IgE via IL-10 and other undefined mechanisms related to B cell trafficking. This study adds to our understanding of why young children may have poor immunity.Nonetheless, praziquantel treatment was shown to reduce percentages of circulating CD193+ B cells lending hope for future vaccine efforts.

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Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

CD193 (CCR3) expression by B cells correlates with reduced IgE production in paediatric schistosomiasis

Onkanga

Sang

Hamilton³

et al. 2023

Parasite Immunology

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ILC3: a case of conflicted identity

Koprivica,

Stanisavljević,

Mićanović

et al. 2023

Front. Immunol.

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Innate lymphoid cells type 3 (ILC3s) are the first line sentinels at the mucous tissues, where they contribute to the homeostatic immune response in a major way. Also, they have been increasingly appreciated as important modulators of chronic inflammatory and autoimmune responses, both locally and systemically. The proper identification of ILC3 is of utmost importance for meaningful studies on their role in immunity. Flow cytometry is the method of choice for the detection and characterization of ILC3. However, the analysis of ILC3-related papers shows inconsistency in ILC3 phenotypic definition, as different inclusion and exclusion markers are used for their identification. Here, we present these discrepancies in the phenotypic characterization of human and mouse ILC3s. We discuss the pros and cons of using various markers for ILC3 identification. Furthermore, we consider the possibilities for the efficient isolation and propagation of ILC3 from different organs and tissues for in-vitro and in-vivo studies. This paper calls upon uniformity in ILC3 definition, isolation, and propagation for the increased possibility of confluent interpretation of ILC3’s role in immunity.

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Expression of CD117 (c-Kit) on Circulating B Cells in Pediatric Schistosomiasis

Abstract: Few B cells express CD27, the primary marker for memory B cells, in pediatric schistosomiasis, suggesting B cell malfunction. This study further demonstrates unexpected high expression of CD117 on circulating B cells in children highly exposed to Schistosoma mansoni infectious larvae.

Cited by 2 publications

References 57 publications

CD193 (CCR3) expression by B cells correlates with reduced IgE production in paediatric schistosomiasis

CD193 (CCR3) expression by B cells correlates with reduced IgE production in paediatric schistosomiasis

ILC3: a case of conflicted identity

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