Expression of carbonic anhydrase II gene in early brain cells as revealed by in situ hybridization and immunohistochemistry

Vitry, F. De; Gomès, Danielle; Rataboul, Pierre; Dumas, Sylvie; Hillion, Joëlle; Catelon, J.; Delaunoy, J. P.; Tixier‐Vidal, A.; Dupouey, P.

doi:10.1002/jnr.490220203

Cited by 9 publications

References 28 publications

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Effects of Diuretics on Renal Function

Puschett

Winaver

1992

Comprehensive Physiology

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The sections in this article are: Diuretic Effects on Renal Hemodynamics Drugs That Primarily Alter Glomerular Function Drugs That Primarily Alter Tubular Function Diuretic Effects on Intrarenal Blood Flow Distribution Diuretic Interactions with the Renin–Angiotensin‐Aldosterone system Diuretic Interactions with Prostaglandin Synthesis and with the Inhibition of Prostaglandin Synthetase Nephron Sites of Action and Effects of Diuretics on Solute Excretion Review and Comparison of Methodologies Utility of the Clearance Technique for the Study of Diuretic Site and Mechanism of Action in Man Diuretic Effects on the Excretion of Specific Solutes Cellular and Molecular Actions of Diuretics Carbonic Anhydrase Inhibitors Loop Diuretics Thiazides Mineralcorticoid Antagonists Amiloride

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Effects of Diuretics on Renal Function

Puschett

Winaver

1992

Comprehensive Physiology

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Double‐labeling in situ hybridization analysis of mRNAs for carbonic anhydrase II and myelin basic protein: Expression in developing cultured glial cells

Ghandour

Skoff

1991

Glia

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KEY WORDSOligodendrocyte ABSTRACTWe applied in situ hybridization to analyze the location and the developmental changes in the distribution of the transcripts for carbonic anhydrase I1 (CAII) and myelin basic protein (MBP) in mouse primary cultured glial cells. Both mRNAs were localized to the oligodendrocyte using double-labeling in situ hybridization. No evidence for CAII transcripts in astrocytes was obtained, indicating that CAII is expressed only by oligodendrocytes in normal rodent glia. As early as 48 h after plating, CAII and MBP mRNAs are present in a few, small round cells. Message is present 2-4 days before levels of these proteins can be detected in similar primary glial cultures.The intensity of labeling for MBP and CAII mRNA positive cells increases significantly during the second week but then decreases after the end of the third week.Only the oligodendrocyte perikaryon and a few processes are positive during the first week. In contrast, at 14 days, a large number of cell processes in addition to the cell bodies are heavily stained for both mRNAs. Both mRNAs could be detected far away from the cell body, up to 250 pm in some cell processes. Some segments on a cell process accumulate higher levels of mRNA than other areas. These areas may correspond to the accumulation of free ribosomes and to starting points for the membrane sheets elaborated by cultured oligodendrocytes. The developmental profile for timing and distribution of these two messages mimics closely their in situ pattern.

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Identification of a human glial fibrillary acidic protein cDNA: A tool for the molecular analysis of reactive gliosis in the mammalian central nervous system

Rataboul

Biguet

Vernier

et al. 1988

J of Neuroscience Research

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Two clones encoding human glial fibrillary acidic protein (GFAP) were isolated from a human astrocytoma cDNA library. The clones pHGFAP1 and pHGFAP2 were selected by the combined use of differential colony hybridization and hybridization-selection technique with polyclonal anti GFAP antiserum. The longer one, pHGFAP1, encompasses 3.0 kb and includes the 1.8 kb long 3' untranslated region specific to the human mRNA. Sequence data disclosed an extensive homology within the coding region of human and mouse GFAP cDNAs even in the end domains. Blot hybridization analysis of RNAs from human, rat and mouse brain revealed a single GFAP mRNA species of 3.1, 2.8 and 2.7 kb respectively and Southern blot experiments indicated that this mRNA is most probably transcribed from a unique gene. In situ hybridization performed with biotinylated probes on cultured mouse brain cells suggests both the sorting and the transport of GFAP mRNA throughout the cytoplasm and processes of the astrocytes. As a model of reactive gliosis secondary to degenerative disorders, 6-hydroxydopamine (6-OHDA) lesion of the substantia nigra in the rat was performed. GFAP mRNA increased 1.4 fold in the ipsilateral striatum on day 10 after the lesion. It then declined to the control level 4 months later contrasting with the lower and more sustained increase in preproenkephalin (PPE) mRNA. The interspecies cross-reactivity of the HGFAP probes make them useful as a tool for the molecular analysis of reactive gliosis in various experimental models.

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Expression of carbonic anhydrase II gene in early brain cells as revealed by in situ hybridization and immunohistochemistry

Cited by 9 publications

References 28 publications

Effects of Diuretics on Renal Function

Effects of Diuretics on Renal Function

Double‐labeling in situ hybridization analysis of mRNAs for carbonic anhydrase II and myelin basic protein: Expression in developing cultured glial cells

Identification of a human glial fibrillary acidic protein cDNA: A tool for the molecular analysis of reactive gliosis in the mammalian central nervous system

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