Expression of c-fos in gastric myenteric plexus and spinal cord of rats with cervical spondylosis

Song, Pei-Song; Kangmei, Kong; Niu, Chunyan; Wei-li, QI; Wu, Ling‐Fei; Wang, Xinjia; Han, Weiwei; Huang, Keng Yen; Chen, Ze-Feng

doi:10.3748/wjg.v11.i4.529

Cited by 3 publications

(6 citation statements)

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“…The FOS gene, whose full name is FOS proto-oncogene, is also named cfos. An immediate-early gene, the FOS gene has been shown to play a key role in cell growth, proliferation and differentiation [5][6][7] Kegg Enrichment Analysis A total of seventeen biological pathways were signi cantly enriched in the DEGs (Table S4), and further analysis revealed that the FOS gene was signi cantly enriched in the pertussis, leishmaniasis, Chagas disease (American trypanosomiasis), herpes simplex infection, and MAPK signaling pathways. Furthermore, the MAPK signaling pathway (Fig.…”

Section: De In the Skin Of Tan Sheep In The Birth And Er-mao Periodsmentioning

confidence: 99%

Skin Transcriptome Analysis Identifies the Key Genes Underlying Fur Development in Chinese Tan Sheep in the Birth and Er-mao Periods

Li¹,

Hui²,

Ma³

et al. 2020

Preprint

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BackgroundHairfollicle development in Tan sheepdiffers significantly between the birth and Er-mao periods, but the underlying molecular mechanism is still unclear.MethodsWe profiled the skin transcriptomes of Tan sheepinthe birth and Er-mao periods via RNA-seq technology. TheTan sheep examined consisted ofthree sheep inthe birth period and threesheep inthe Er-mao period. ResultsA total of 364 differentially expressed genes (DEGs) in the skin of Tan sheepbetweenthe birth period and the Er-mao period were identified, among which 168 were upregulated and 196 were downregulated. Interestingly, the FOS proto-oncogene(FOS)(fold change=22.67, P value=2.15*10^-44)was the most significantly differentially expressed gene. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis found that the FOS gene was significantly enriched in the signaling pathway related to hair follicle development. Immunohistochemical analysis showed that the FOS gene was expressed in the skin of Chinese Tan sheep at the birth and Er-mao periods,with abnormally high expression in the Er-mao period. Conclusions Our findings suggest that the FOS gene promotes hair follicle development in Tan sheep.

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Section: De In the Skin Of Tan Sheep In The Birth And Er-mao Periodsmentioning

confidence: 99%

Skin Transcriptome Analysis Identifies the Key Genes Underlying Fur Development in Chinese Tan Sheep in the Birth and Er-mao Periods

Li¹,

Hui²,

Ma³

et al. 2020

Preprint

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“…The method for gastric neuronal counterstaining was adapted from previous studies [6,18,19] . c-Fos cells were counted under microscopy in 25 ganglia from each antral preparation and expressed as a mean percentage count per myenteric ganglion.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…Immunohistochemical staining was car ried out using the avidin-biotin complex method as previously described [6,17] . The concentrations for rabbit anti-IL-1β, rabbit anti-caspase-3 and rabbit polyclonal anti-c-fos primary antibodies were 1:200, 1:200 (Sigma, Genetimes Technolog y Inc.) and 1:100 000 (diluted in NGS-T-PBS).…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…c-Fos as the third messenger, which regulates the target gene, provides a reliable and direct method to study the mechanism of functional gastrointestinal diseases [5] . Our previous study [6] reported that the c-Fos expression in the gastric myenteric plexus was dramatically associated with c-Fos expression of the spinal cord in the rats with cervical spondylosis. It is the sympathetic nerve that results in the c-Fos expression both in the spinal cord and the gastric myenteric plexus in cer vical spondylosis and this sug gests that the gastrointestinal function may be affected by cervical spondylosis.…”

Section: Introductionmentioning

confidence: 99%

“…In the present study, after establishing the cervical spondylosis model of rats according to a previously described method [6] , the cord and stomach were collected at 3 mo and 5 mo to determine the expression of c-Fos, caspase-3 and IL-1β in the cervical cord and gastric antrum by immunohistochemistry and/or Western blot.…”

Section: Introductionmentioning

confidence: 99%

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A preliminary study of neck-stomach syndrome

Song

Xu²,

Ding³

et al. 2007

WJG

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AIM:To determine the expression of c-Fos, caspase-3 and interleukin-1β (IL-1β) in the cervical cord and stomach of rats with cervical spondylosis, to analyze their relationship, and to offer an explanation of one possible cause for functional dyspepsia (FD) and irritable bowel syndrome (IBS) caused by cervical spondylosis. METHODS:The cervical spondylosis model in rats was established by destroying the stability of cervical posterior column. The cord segments C4-6 and gastric antrum were collected 3 mo and 5 mo after the operation. Rats with the sham operation were used as controls. The expressions of c-Fos, caspase-3 and IL-1β in the cervical cord and gastric antrum were determined by immunohistochemistry and/or Western blot. RESULTS:Immunohistochemical staining showed a few c-Fos, caspase-3 and IL-1β-positive cells in the cervical cord and antrum in the control. There was a significant increase in c-Fos, caspase-3 and IL-1β expression in model groups compared to the control groups at 3 mo and 5 mo after operation. More importantly, there was a significant (P < 0.05) increase in c-Fos, caspase-3 and IL-1β expression in the model group rats at 3 mo compared to those at 5 mo after the operation (c-Fos:11.20 ± 2.26 vs 27.68 ± 4.36 in the cervical cord, 11.3 ± 2.3 vs 29.3 ± 4.6 in the gastric antrum; caspase-3: 33.83 ± 3.71 vs 36.32 ± 4.01 in the cervical cord, 13.23 ± 3.21 vs 26.32 ± 4.01 in the gastric antrum; IL-1β: 42.06 ± 2.95 vs 45.91 ± 3.98 in the cervical cord, 26.56 ± 2.65 vs 32.01 ± 2.98 in the gastric antrum). Western blot analysis showed time-dependent changes of caspase-3 and IL-1β protein in the cervical cord and gastric antrum of rats with cervical spondylosis; there was no significant expression of caspase-3 and IL-1β protein in the control group at 3 mo and 5 mo after the sham operation, whereas there was a significant difference in caspase-3 and IL-1β protein levels between the model group rats followed up for 3 mo and those for 5 mo (P < 0.05). CONCLUSION:There is a significant association of c-Fos, caspase-3 and IL-1β expressions in the gastric antrum with that in the spinal cord in rats with cervical spondylosis, suggesting that the gastrointestinal function may be affected by cervical spondylosis.

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Skin transcriptome analysis identifies the key genes underlying fur development in Chinese Tan sheep in the birth and Er-mao periods

He²,

Hui

et al. 2022

Gene

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BackgroundHairfollicle development in Tan sheepdiffers signi cantly between the birth and Er-mao periods, but the underlying molecular mechanism is still unclear. MethodsWe pro led the skin transcriptomes of Tan sheepinthe birth and Er-mao periods via RNA-seq technology. TheTan sheep examined consisted ofthree sheep inthe birth period and threesheep inthe Er-mao period. ResultsA total of 364 differentially expressed genes (DEGs) in the skin of Tan sheepbetweenthe birth period and the Er-mao period were identi ed, among which 168 were upregulated and 196 were downregulated. Interestingly, the FOS proto-oncogene(FOS)(fold change=22.67, P value=2.15*10^-44)was the most signi cantly differentially expressed gene. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis found that the FOS gene was signi cantly enriched in the signaling pathway related to hair follicle development. Immunohistochemical analysis showed that the FOS gene was expressed in the skin of Chinese Tan sheep at the birth and Er-mao periods,with abnormally high expression in the Er-mao period. ConclusionsOur ndings suggest that the FOS gene promotes hair follicle development in Tan sheep.

show abstract

Expression of c-fos in gastric myenteric plexus and spinal cord of rats with cervical spondylosis

Cited by 3 publications

References 30 publications

Skin Transcriptome Analysis Identifies the Key Genes Underlying Fur Development in Chinese Tan Sheep in the Birth and Er-mao Periods

Skin Transcriptome Analysis Identifies the Key Genes Underlying Fur Development in Chinese Tan Sheep in the Birth and Er-mao Periods

A preliminary study of neck-stomach syndrome

Skin transcriptome analysis identifies the key genes underlying fur development in Chinese Tan sheep in the birth and Er-mao periods

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