Expression of brush border enzymes in response to lead exposure in rat intestine

Kapur, Priya; Kaur, Kamaljit; Mahmood, Akhtar

doi:10.1002/jat.1079

Cited by 5 publications

(6 citation statements)

References 20 publications

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“…The resulting increased ratio of mature epithelia cells compared with immature cells on the villi has been cited as a reason for altered brush border enzyme level noted in some studies 26 . In addition, the changes in enzyme activities of enterocytes are in agreement with the findings with microvillus membrane in ethanol‐fed rats 28 . This may suggest that the underlying mechanism of enzyme expression is distinct in differentiating enterocytes.…”

Section: Discussionsupporting

confidence: 87%

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Functional and morphological alterations in small intestine mucosa of chronic alcoholics

Bhonchal¹,

Nain²,

Prasad³

et al. 2008

J of Gastro and Hepatol

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Section: Discussionsupporting

confidence: 87%

“…26 In addition, the changes in enzyme activities of enterocytes are in agreement with the findings with microvillus membrane in ethanol-fed rats. 28 This may suggest that the underlying mechanism of enzyme expression is distinct in differentiating enterocytes.…”

Section: Discussionmentioning

confidence: 99%

Functional and morphological alterations in small intestine mucosa of chronic alcoholics

Bhonchal¹,

Nain²,

Prasad³

et al. 2008

J of Gastro and Hepatol

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“…Antisera against the LAP showed reactivity at 80 kDa, which served as an internal control. LAP is a membrane-bound aminopeptidase present in intestinal brush border membrane, whose activity has been found to be unaffected by chronic ethanol feeding [20,21]. The expression of the RFC protein was observed to be three-fold higher in BBMV from the control group when compared with the chronic, ethanol-fed group (Fig.…”

Section: Expression Of the Rfc At The Brush Border Membrane Surfacementioning

confidence: 80%

Role of signaling pathways in the regulation of folate transport in ethanol-fed rats

Hamid¹,

Kaur²

2009

The Journal of Nutritional Biochemistry

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“…Antisera against the leucine aminopeptidase (LAP) showed reactivity at 80 kDa, which served as an internal control. LAP is a membrane‐bound aminopeptidase whose activity has been found to be unaffected by chronic ethanol feeding [18,19]. The expression of the RFC protein was observed to be 2.3‐fold higher in BBMVs from the control group relative to those from the chronic ethanol‐fed group (Fig.…”

Section: Resultsmentioning

confidence: 98%

Down‐regulation of reduced folate carrier may result in folate malabsorption across intestinal brush border membrane during experimental alcoholism

et al. 2007

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Folate plays a critical role in maintaining normal metabolic, energy, differentiation and growth status of all mammalian cells. The intestinal folate uptake is tightly and diversely regulated, and disturbances in folate homeostasis are observed in alcoholism, attributable, in part, to intestinal malabsorption of folate. The aim of this study was to delineate the regulatory mechanisms of folate transport in intestinal absorptive epithelia in order to obtain insights into folate malabsorption in a rat model of alcoholism. The rats were fed 1 g.kg(-1) body weight of ethanol daily for 3 months. A reduced uptake of [(3)H]folic acid in intestinal brush border membrane was observed over the course of ethanol administration for 3 months. Folate transport exhibited saturable kinetics and the decreased intestinal brush border membrane folate transport in chronic alcoholism was associated with an increased K(m) value and a low V(max) value. Importantly, the lower intestinal [(3)H]folic acid uptake in ethanol-fed rats was observed in all cell fractions corresponding to villus tip, mid-villus and crypt base. RT-PCR analysis for reduced folate carrier, the major folate transporter, revealed that reduced folate carrier mRNA levels were decreased in jejunal tissue derived from ethanol-fed rats. Parallel changes were observed in reduced folate carrier protein levels in brush border membrane along the entire crypt-villus axis. In addition, immunohistochemical staining for reduced folate carrier protein showed that, in alcoholic conditions, deranged reduced folate carrier localization was observed along the entire crypt-villus axis, with a more prominent effect in differentiating crypt base stem cells. These changes in functional activity of the membrane transport system were not caused by a general loss of intestinal architecture, and hence can be attributed to the specific effect of ethanol ingestion on the folate transport system. The low folate uptake activity observed in ethanol-fed rats was found to be associated with decreased serum and red blood cell folate levels, which might explain the observed jejunal genomic hypomethylation. These findings offer possible mechanistic insights into folate malabsorption during alcoholism.

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Expression of brush border enzymes in response to lead exposure in rat intestine

Cited by 5 publications

References 20 publications

Functional and morphological alterations in small intestine mucosa of chronic alcoholics

Functional and morphological alterations in small intestine mucosa of chronic alcoholics

Role of signaling pathways in the regulation of folate transport in ethanol-fed rats

Down‐regulation of reduced folate carrier may result in folate malabsorption across intestinal brush border membrane during experimental alcoholism

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