Expression of biologically active viral satellite RNA from the nuclear genome of transformed plants

Baulcombe, David C.; Saunders, Graham R.; Bevan, Michael W.; Mayo, M. A.; Harrison, B. D.

doi:10.1038/321446a0

Cited by 243 publications

(128 citation statements)

References 22 publications

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“…However, this approach may involve environmental hazards (Nelson et al, 1988) which could be eliminated through the expression of disabled forms of satellite RNA that cannot be acquired and transmitted by CMV (Baulcombe et al, 1986) and in which sequences affecting symptom production have been deleted or modified. It is not yet known to what extent At05/188 or A109/191 fit those requirements.…”

Section: Discussionmentioning

confidence: 99%

“…The accession received was designated I 17N' satellite RNA in order to distinguish it from the I17N satellite RNA described by Jacquemond & Lauquin (1988) (see Results section). Satellite eDNA clones were described previously in Devic et al (1989) (Y satellite RNA) and Baulcombe et al (1986) (I17N" satellite RNA). The inocula of CMV-KIN and TAV used as helper viruses were extracts of total nucleic acids from infected plants that were prepared as described by Devic et al (1989).…”

Section: Methodsmentioning

confidence: 99%

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Cucumber mosaic virus satellite RNA (Y strain): analysis of sequences which affect yellow mosaic symptoms on tobacco

Jaegle¹,

Devic

Longstaff

et al. 1990

Journal of General Virology

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Plants infected with cucumber mosaic virus (CMV) (KIN strain) produce a mild mosaic disease on tobacco whereas infections of CMV with satellite RNA (strain Y) cause a severe yellow mosaic. Analysis of recombinant and mutant forms of satellite RNA identified a site (nucleotides 185/186) in the Y satellite RNA that affects the ability to induce the yellow mosaic in combination with CMV but not with tomato aspermy virus. The location of this site with respect to other mutations in the satellite RNA indicated that polypeptides, which may be encoded by the satellite RNA, have no role in induction of yellow mosaic symptoms. The symptom induction is therefore an effect of the satellite RNA on the host plant with the intervention of the helper virus. In the course of the mutation analysis of satellite RNA we detected several secondary mutations which arose in planta. Two of these were deletions of more than 80 nucleotides. Other forms of mutant satellite RNA were non-functional even though the modifications involved nucleotides completely within the large secondary deletions. These data imply complex intramolecular interactions in the satellite RNA.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Cucumber mosaic virus satellite RNA (Y strain): analysis of sequences which affect yellow mosaic symptoms on tobacco

Jaegle¹,

Devic

Longstaff

et al. 1990

Journal of General Virology

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“…Into the Bam HI site of the expression cassette pRokl [3] we cloned the hygromycin phosphotransferase (HPTII) gene (a 1 kb Barn HI fragment with a single ATG to improve the translation efficiency; van den Elzen, unpublished) and modified the resulting plasmid by filling in the Bam HI site between the HPTII gene and the nopaline synthase (nos) polyadenylation signal. After the cloning of plasmid pUC18, containing the bases 2640 (SphI)-6130(XhoI) from the T-DNA of pTiAch5 [ 12] in the Hind III site, the plasmid has been renamed pTT218 ( Fig.…”

Section: Phenotypic Assay Vectors For Tam3 and Acmentioning

confidence: 99%

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

et al. 1989

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Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Ac excises from the HPTII gene with comparable frequencies (30~o) in both plant species. When the /~-glucuronidase (GUS) gene was used to detect transposition of Tam3, a significantly lower excision frequency (13~o) was found in both plant species. It could be shown that deletion of parts of the transposable elements Tam3 and Ac, removing either one of the terminal inverted repeats (TIR) or part of the presumptive transposase coding region, abolished the excision from the marker genes. This demonstrates that excision of the transposable element Tam3 in heterologous plant species, as documented for the autonomous element Ac, also depends on both properties. Southern blot hybridization shows the expected excision pattern and the reintegration of Tam3 and Ac elements into the genome of tobacco plants.

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“…The vector was constructed in two steps. First, a I kb BamHI fragment containing HPT II was inserted into the BamHI site of the expression cassette pRoM (Baulcombe et al 1986). After filling-in the BamHI site between HPT II and the polyadenylation signal, the resulting plasmid was named pTT212.…”

Section: Methodsmentioning

confidence: 99%

Ac-induced disruption of the doubleDs structure in tomato

et al. 1991

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Expression of biologically active viral satellite RNA from the nuclear genome of transformed plants

Cited by 243 publications

References 22 publications

Cucumber mosaic virus satellite RNA (Y strain): analysis of sequences which affect yellow mosaic symptoms on tobacco

Cucumber mosaic virus satellite RNA (Y strain): analysis of sequences which affect yellow mosaic symptoms on tobacco

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

Ac-induced disruption of the doubleDs structure in tomato

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