Ac-induced disruption of the doubleDs structure in tomato

Rommens, Caius M.; Biezen, Erik A. van der; Ouwerkerk, Pieter B.F.; Nijkamp, H. J. J.; Hille, Jacques

doi:10.1007/bf00260639

Cited by 9 publications

(7 citation statements)

References 19 publications

(16 reference statements)

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“…The cDNA was ligated to noncomplementary EcoRI/BstXI adaptors (Invitrogen; Sanbio) and separated from nonligated adaptors on Sepharose CL 4B (Pharmacia) spin columns. The fractions containing cDNA larger than 500 bp were used for ligation into pCDM8 vector digested with BstXI (Seed 1987), and transformed to E. coli MC1061/P3 by electroporation as described by Rommens et al (1991). The library, 7 × 105 clones, was divided into nine fractions, grown overnight, frozen glycerol cultures prepared and DNA isolated by Qiagen tip-500 columns (Qiagen; Westburg, Leusden, The Netherlands) according to the supplier.…”

Section: Construction Of a Um-scc-22a Cdna Library In Pcdm8mentioning

confidence: 99%

The human E48 antigen, highly homologous to the murine Ly-6 antigen ThB, is a GPI-anchored molecule apparently involved in keratinocyte cell-cell adhesion.

Brakenhoff¹,

Gerretsen²,

Knippels³

et al. 1995

The Journal of Cell Biology

125

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Abstract. The E48 antigen, a putative human homologue of the 20-kD protein present in desmosomal preparations of bovine muzzle, and formerly called desmoglein III (dg4), is a promising target antigen for antibody-based therapy of squamous cell carcinoma in man. To anticipate the effect of high antibody dose treatment, and to evaluate the possible biological involvement of the antigen in carcinogenesis, we set out to molecularly characterize the antigen. A cDNA clone encoding the E48 antigen was isolated by expression cloning in COS cells. Sequence analysis revealed that the clone contained an open reading frame of 128 amino acids, encoding a core protein of 13,286 kD. Database searching showed that the E48 antigen has a high level of sequence similarity with the mouse ThB antigen, a member of the Ly-6 antigen family. Phosphatidylinositol-specific (PI-specific) phospholipase-C treatment indicated that the E48 antigen is glycosylphosphatidylinositol-anchored (GPI-anchored) to the plasma membrane. The gene encoding the E48 antigen is a single copy gene, located on human chromosome 8 in the 8q24-qter region. The expression of the gene is confined to keratinocytes and squamous tumor cells. The putative mouse homologue, the ThB antigen, originally identified as an antigen on cells of the lymphocyte lineage, was shown to be highly expressed in squamous mouse epithelia. Moreover, the ThB expression level is in keratinocytes, in contrast to that in lymphocytes, not mouse strain related. Transfection of mouse SV40-polyoma transformed mouse NIH/3T3 cells with the E48 cDNA confirmed that the antigen is likely to be involved in cell-cell adhesion.

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Section: Construction Of a Um-scc-22a Cdna Library In Pcdm8mentioning

confidence: 99%

The human E48 antigen, highly homologous to the murine Ly-6 antigen ThB, is a GPI-anchored molecule apparently involved in keratinocyte cell-cell adhesion.

Brakenhoff¹,

Gerretsen²,

Knippels³

et al. 1995

The Journal of Cell Biology

125

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“…UC82B or Moneymaker respectively by Agrobacterium-mediated transformation, as described [32]. Tomato transformants containing stabilized Ac with a GUS reporter (SLJ 10512) were kindly provided by Dr J.D.G.…”

Section: Transgenic Plantsmentioning

confidence: 99%

Single-site manipulation of tomato chromosomes in vitro and in vivo using Cre-lox site-specific recombination

et al. 1996

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With the aim of developing new techniques for physical and functional genome analysis, we have introduced the Cre-lox site-specific recombination system into the cultivated tomato (Lycopersicon esculentum). Local transposition of a Ds(lox) transposable element from a T-DNA(lox) on the long arm of chromosome 6 was used to position pairs of lox sites on different closely linked loci. In vitro Cre-lox recombination between chromosomal lox sites and synthetic lox oligonucleotides cleaved the 750 Mb tomato genome with 34 bp specificity to release unique 65 kb and 130 kb fragments of chromosome 6. Parallel in vitro experiments on Saccharomyces cerevisiae chromosomes show the efficiency of cleavage to be 50% per chromosomal lox site at maximum. By expressing the Cre recombinase in tomato under control of a constitutive CaMV 35S promoter, efficient and specific somatic and germinal in planta inversion of the 130 kb fragment is demonstrated. The combined use of in vitro and in vivo recombination on genetically mapped lox sites will provide new possibilities for long range restriction mapping and in vivo manipulation of selected tomato genome segments.

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“…Tomato plants (Lycopersicon esculentum HW61) were transformed with an Agrobacteriurn strain containing the binary plasmid vector pTT283 (with a 7.8 kb Ds element between the borders of the T-DNA) [28], as described previously [27]. To induce transposition of Ds, transformants were crossed with a transgenic tomato plant containing an active Ac element.…”

Section: Plant Materialsmentioning

confidence: 99%

“…To induce transposition of Ds, transformants were crossed with a transgenic tomato plant containing an active Ac element. Original sites of Ac were studied using a transgenic plant harbouring an intact T-DNA copy of pTT252 [27]. This T-DNA carries Ac with 18 and 35 bp of flanking maize waxy sequences [15] inserted between the NPT II gene (at the right-border side) and the bacterial plasmid pB R322 (at the left-border side).…”

Section: Plant Materialsmentioning

confidence: 99%

Characterization of theAc/Ds behaviour in transgenic tomato plants using plasmid rescue

et al. 1992

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We describe the use of plasmid rescue to facilitate studies on the behaviour of Ds and Ac elements in transgenic tomato plants. The rescue of Ds elements relies on the presence of a plasmid origin of replication and a marker gene selective in Escherichia coli within the element. The position within the gehome of modified Ds elements, rescued both before and after transposition, is assigned to the RFLP map of tomato. Alternatively to the rescue of Ds elements equipped with plasmid sequences, Ac elements are rescued by virtue of plasmid sequences flanking the element. In this way, the consequences of the presence of an (active) Ac element on the D N A structure at the original site can be studied in detail. Analysis of a library of Ac elements, rescued from the genome of a primary transformant, shows that Ac elements are, infrequently, involved in the formation of deletions. In one case the deletion refers to a 174 bp genomic D N A sequence immediately flanking Ac. In another case, a 1878 bp internal Ac sequence is deleted.

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Ac-induced disruption of the doubleDs structure in tomato

Cited by 9 publications

References 19 publications

The human E48 antigen, highly homologous to the murine Ly-6 antigen ThB, is a GPI-anchored molecule apparently involved in keratinocyte cell-cell adhesion.

The human E48 antigen, highly homologous to the murine Ly-6 antigen ThB, is a GPI-anchored molecule apparently involved in keratinocyte cell-cell adhesion.

Single-site manipulation of tomato chromosomes in vitro and in vivo using Cre-lox site-specific recombination

Characterization of theAc/Ds behaviour in transgenic tomato plants using plasmid rescue

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