Expression of Bcl-2 in Breast Cancer: Correlation with Clinicopathological Characteristics and Survival

Čečka, Filip; Hornychová, Helena; Melichar, Bohuslav; Ryška, Aleš; Jandı́k, P; Mergancová, J; Klozová-Urminská, Hana

doi:10.14712/18059694.2017.11

Cited by 8 publications

(9 citation statements)

References 48 publications

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“…Further, Filip Čečka et al . 28 showed no significant relationship between BCL-2 expression with tumor grade and primary tumors, which concurs with our findings herein. The observed correlations among clinical-histopathological features (cancer stage vs cancer grade; cancer grade vs nodule status; T stage vs cancer stage/cancer grade) reported in the current study could be explained by the fact that the larger the primary tumor the greater the number of lymph nodes that will be affected in advanced cancer stage and cancer grade 29 .…”

Section: Discussionsupporting

confidence: 92%

Ki-67, p53 and BCL-2 Expressions and their Association with Clinical Histopathology of Breast Cancer among Women in Tanzania

Mansouri

Mnango

Magorosa

et al. 2019

Sci Rep

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This study associated Ki-67, p53, and BCL-2 markers with clinical histopathological (CH) features using currently available limited data on these markers in Tanzania. Retrospective chart review study was conducted among females with confirmed breast cancer (BC) at Muhimbili National Hospital in Tanzania between 2016 and 2017. Inclusion criteria were met by 76 patients with a mean age of 51.32 ± 14.28 years. Of these, 86.4% were stage III and IV, whereas 83.5% cases had grade 2 and grade 3. Upon immunostaining, 85.5% and 57.9% were Ki-67 and BCL-2 positive respectively. Log-linear analysis showed no statistically significant association among biomarkers expression and CH features. However, multinomial linear regression showed higher possibility for association between high expression of Ki-67, low expression of p53 and high expression of BCL-2 with age, grade, stage and tumor (T) stage. BCL-2 was positively correlated with Ki-67 expression contrary to p53, which was negatively correlated with BCL-2. Conclusively, there is evidence of correlation between the studied markers with CH features. However, studies with larger sample sizes will likely reveal significant associations that will validate the role of these markers as tools for evaluating treatment response in individualized therapeutic schemes in Tanzania.

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Section: Discussionsupporting

confidence: 92%

Ki-67, p53 and BCL-2 Expressions and their Association with Clinical Histopathology of Breast Cancer among Women in Tanzania

Mansouri

Mnango

Magorosa

et al. 2019

Sci Rep

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“…Studies have shown that changes in the ratio of Bcl-2 to Bax can regulate apoptosis. Bcl-2 overexpression has anti-apoptotic effects; in contrast, Bax overexpression stimulates apoptosis (24). In the present study, we found that Bcl-2 levels were significantly decreased and Bax levels were significantly increased in p-CA-treated A375 and B16 cells.…”

Section: Discussionsupporting

confidence: 47%

The Anti-tumor Effects of p-Coumaric Acid on Melanoma A375 and B16 Cells

et al. 2020

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Background: Existing research shows that p-coumaric acid (p-CA) can inhibit the proliferation of a variety of tumor cells in vitro. However, there are no reports on the anti-tumor effects of p-CA on melanoma cells. In this study, the inhibitory effects of p-CA on mouse melanoma B16 and human melanoma A375 cells are reported, and the related mechanisms are investigated. Methods: CCK-8 assay was used to detect the effects of p-CA on cell vitality, colony formation assay was used to observe the effects on cell proliferation, Hoechst 33,258 staining was used to observe the morphology of apoptotic cells, flow cytometry was used to detect the effects on apoptosis and the cell cycle, and western blot was used to measure the levels of cell cycle-and apoptosis-related signaling pathway proteins. Results: p-CA significantly inhibits cell proliferation of A375 and B16 cells in a dose-dependent manner and obviously induced cell morphological changes. p-CA arrested A375 cells in the S phase by downregulating the cell cycle-related proteins Cyclin A and CDK2, and arrested B16 cells in the G0-G1 phase through downregulating the cell cycle-related proteins Cyclin E and CDK2. In addition, p-CA significantly promoted apoptosis of A375 and B16 cells. Furthermore, p-CA significantly upregulated the levels of Apaf1 and Bax and downregulated the levels of Bcl-2, and subsequently increased the levels of cytoplasmic cytochrome c (Cyto-c), cleaved caspase-3, and cleaved caspase-9, leading to apoptosis in A375 and B16 cells. Conclusion: p-CA can significantly inhibit the proliferation of human and mouse melanoma cells in vitro. Our research is a step in the development of anti-melanoma drugs.

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“…Cecka, et al [2] did study on expression of BCl2 in 57 females suffering from primary Breast cancer who were treated with neoadjuvant chemotherapy. The immunohistochemistry (IHC) for BCl2 were performed either on the surgical specimens or core cut biopsies with Streptavidin and Biotin method with peroxidise detection system.…”

Section: Discussionmentioning

confidence: 99%

“…The modern day practice of oncopathology revolves more around predictive prognostic markers that would enable the appropriate adjuvant therapies and management of cancer. One of the challenges in breast cancer management is to accurately predict the outcome for each patient, so that the patients will be benefitted from adjuvant therapies or by appropriate surgical interventions [1,2].…”

Section: Introductionmentioning

confidence: 99%

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A Study of Bcl-2 Immuno-expression in Invasive Ductal Carcinoma of Breast and it’s Correlation with Molecular Sub-types

Pandey

Bhake

Vagha

2021

JPRI

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Background: The incidence of Breast cancers has overtaken the cervical cancers amongst Indian females. Invasive Ductal carcinoma is reported to be the most common form of breast cancer. Bcl-2 has been studied extensively as a common factor in the pathogenesis of solid tumours including breast cancer. Bcl-2 is an anti-apoptotic protein normally expressed in mammary tissue and is up-regulated by oestrogen in breast cancer through direct consequence of transcriptional induction. The present study attempts to look into Bcl-2 immunoexpression as a key parameter for predicting the treatment outcomes and recurrence of Invasive Ductal Carcinoma. Methodology : This will be an observational study conducted in Department of Pathology, JNMC, Wardha. The study will include clinicopathological detailing of 50 mastectomy specimens of Invasive ductal carcinoma, detailed sectioning of tumour tissues, histopathological BR-grading and molecular subtyping using Bcl-2 immunohistochemistry. Expected Results: A significant correlation is expected between Bcl-2 expression and treatment outcomes and recurrence of Invasive Ductal Carcinoma. Conclusion: The conclusion will be drawn based on careful analysis of the results.

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Expression of Bcl-2 in Breast Cancer: Correlation with Clinicopathological Characteristics and Survival

Cited by 8 publications

References 48 publications

Ki-67, p53 and BCL-2 Expressions and their Association with Clinical Histopathology of Breast Cancer among Women in Tanzania

Ki-67, p53 and BCL-2 Expressions and their Association with Clinical Histopathology of Breast Cancer among Women in Tanzania

The Anti-tumor Effects of p-Coumaric Acid on Melanoma A375 and B16 Cells

A Study of Bcl-2 Immuno-expression in Invasive Ductal Carcinoma of Breast and it’s Correlation with Molecular Sub-types

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