Expression of basigin in the early phase of acute myocardial ischemia in rats

Abstract

Abstract. Basigin may be involved in cardiovascular disease. In our previous study, suppression subtractive hybridization results indicated that basigin may be associated with the early phase of acute myocardial ischemia (AMI) within 1 h. However, little is known regarding the expression of basigin in the early phase of AMI. The aim of the present study was to evaluate the temporal and spatial expression patterns of basigin mRNA and protein levels in AMI in rats. We constructed an AMI model in rats that received left anterior descending coronary artery ligation for 0, 15, 30, 60, 120 or 240 min. Real-time quantitative PCR and in situ hybridization (ISH) were conducted to reveal the basigin mRNA levels in the early ischemic myocardium (EIM) and non-ischemic myocardium (NIM). The expression levels of basigin protein were detected using western blot analysis and immunohistochemistry. The expression levels of basigin mRNA and protein significantly changed in the EIM as early as 30 min from ischemia, and the changes continued to be present throughout the ischemic period (P<0.05). The expression levels of basigin mRNA were significantly reduced, whilst those of the protein underwent a significant ~2-fold increase in the EIM. However, there were no significant differences in the basigin mRNA or protein expression levels from 0-240 min in the NIM (P>0.05). We failed to detect a signal for basigin mRNA in the myocardium by ISH. Our findings indicated that basigin may be involved in acute myocardial ischemia following continual ischemia for >30 min.

Sorbin and SH3 Domain‐Containing Protein 2 Is Released From Infarcted Heart in the Very Early Phase: Proteomic Analysis of Cardiac Tissues From Patients

“…10–12 Although some AMI model experiments have attempted to determine the molecular profile alterations associated with short‐term ischemia, elucidation of the acute proteomic changes is challenging if tissues are collected macroscopically. 13–14 …”

Sex Hormones Regulate Rat Hepatic Monocarboxylate Transporter Expression and Membrane Trafficking

“…2000 ng of total RNA was added to each 40 μl cDNA synthesis reaction, which was reverse transcribed to cDNA using iScript Reverse Transcription Supermix (BioRad). Primer sequences, annealing temperatures and product sizes for quantitative real-time PCR are shown in Table 1 (14,15). Primers were validated by running endpoint PCR with 250 nM forward and reverse primers, 1 μl of cDNA and iTaq Universal SYBR Green Supermix (BioRad) following the manufacturer's instructions.…”

Association of NQO1 levels and its genetic polymorphism with susceptibility to methamphetamine dependence

“…In yeast and bacteria, 30%-85% of protein levels can be attributed to changes in mRNA expression, the other 15%-70% variation is explained by posttranscription or posttranslation regulation and measurement errors, only 40% of proteins in multicellular organisms can be explained by mRNA (de Sousa Abreu et al, 2009). Studies have shown that more than 70% of the protein expression is regulated by mRNA, in yeast, while only about half of the protein expression in E. coli is regulated by mRNA (Liu et al, 2013;Lu et al, 2007). Besides, we substituted the NQO1 mRNA levels in PBMCs and plasma protein levels for those of brain tissue, which might be possibly different from that in the brain.…”

“…Studies have shown that more than 70% of the protein expression is regulated by mRNA, in yeast, while only about half of the protein expression in E . coli is regulated by mRNA (Liu et al, 2013 ; Lu et al, 2007 ). Besides, we substituted the NQO1 mRNA levels in PBMCs and plasma protein levels for those of brain tissue, which might be possibly different from that in the brain.…”