Expression of Basigin, an Inducer of Matrix Metalloproteinases, in the Rat Ovary1

Smedts, Anna M.; Curry, Thomas E.

doi:10.1095/biolreprod.104.036145

Cited by 18 publications

(7 citation statements)

References 41 publications

(52 reference statements)

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“…In contrast, basigin mRNA and protein were detectable in both new corpus lutea and corpus lutea from previous cycles in the rat ovary (Smedts and Curry, 2005), suggesting that basigin expression in corpus lutea is species-specific. Still, the ovaries of Bsg-null mutant mice exhibit normal folliculogenesis and corpus lutea (Kuno et al, 1998), as well as normal progesterone levels on day 8 of pseudopregancy (Chen et al unpublished data).…”

Section: Discussionmentioning

confidence: 84%

Expression of basigin in reproductive tissues of estrogen receptor-α or -β null mice

Chen¹,

Bi²,

Nakai³

et al. 2010

Reproduction

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Basigin plays important roles in both male and female reproduction because basigin (Bsg) null male and female mice are infertile. The aim of the present study was to determine whether basigin expression in reproductive organs requires oestrogen receptor (ER) α or ERβ. Expression of basigin protein in the testis, ovary and male and female reproductive tracts was studied in adult wild type, ERα-null (αERKO) and ERβ-null (βERKO) mice by immunohistochemistry and immunoblotting. Basigin mRNA levels in ovary and uterus were examined by quantitative RT-PCR. In females, basigin protein expression was observed mainly in granulosa and interstitial cells of the ovary and epithelial cells of the proximal oviduct in all genotypes. Basigin protein was also expressed in the uterine epithelium at prooestrus and oestrus in WT and βERKO mice but not in αERKO mice. However, a higher level of basigin mRNA was observed in uteri of αERKO mice compared with WT and βERKO mice. In males, basigin was expressed in Leydig cells and all germ cells except spermatogonia in all genotypes. Basigin was present in epithelial cells lining the efferent ductules in WT and βERKO mice but expression was greatly reduced in αERKO mice. In epididymal ducts, basigin expression was observed in epithelial cells in the caput and cauda in all genotypes. These data suggest that expression of basigin protein requires ERα, but not ERβ, in the uterus and efferent ductules, but is independent of ER in the ovary, oviduct, testis and epididymis.

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Section: Discussionmentioning

confidence: 84%

Expression of basigin in reproductive tissues of estrogen receptor-α or -β null mice

Chen¹,

Bi²,

Nakai³

et al. 2010

Reproduction

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“…EMMPRIN is associated with cell growth and adhesion [25], angiogenesis [26,27], chaperone functions [28,29], immune cell activation [30], proMMP-1 activation [31], MMP induction [32,33], and ECM degradation and remodeling [34]. EMMPRIN is highly associated with the menstrual cycle [35,36], estrous cycle, and pregnancy in mice [17] and rats [37], as well as ovulation and luteogenesis [38]. In cow, EMMPRIN may be involved in endometrial remodeling for implantation same as those in other species because recent reports showed that MMP and ECM-related molecules including EMMPRIN expressed in bovine endometrium and placenta [10,14].…”

Section: Introductionmentioning

confidence: 99%

Expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and its related extracellular matrix degrading enzymes in the endometrium during estrous cycle and early gestation in cattle

Mishra

Kizaki

Koshi

et al. 2010

Reprod Biol Endocrinol

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BackgroundExtracellular matrix metalloproteinase inducer (EMMPRIN) regulates several biological functions involving the modulation of cell behaviors via cell-cell and cell-matrix interactions. According to its diverse functions, we hypothesized that EMMPRIN may play an important role in endometrial remodeling and establishment of pregnancy in cow.MethodsIn this study, endometrial tissues from the cyclic cows during before ovulation, after ovulation and middle of estrous cycle; and pregnant endometrial tissues from Day 19 to 35 of gestation have been used. Expression of mRNA was analyzed by RT-PCR, qPCR and in situ hybridization whereas protein expression by immunohistochemistry and western blot analysis.ResultsEMMPRIN mRNA was expressed in both cyclic and pregnant endometrium and significantly higher in the endometrium at Day 35 of gestation than the cyclic endometrium. In Western blot analysis, an approximately 65 kDa band was detected in the endometrium, and approximately 51 kDa in the cultured bovine epithelial cells and BT-1 cells, respectively. Both in situ hybridization and immunohistochemistry data showed that EMMPRIN was primarily expressed in luminal and glandular epithelium with strong staining on Day 19 conceptus. At Day 19 of gestation, expression of EMMPRIN mRNA on luminal epithelium was decreased than that observed at middle of estrous cycle, however, on Day 30 of gestation, slightly increased expression was found at the site of placentation. Expression of matrix metalloproteinase-2 (MMP-2) and MMP-14 mRNA were mainly detected in stroma and their expression also decreased at Day 19 of gestation however it was also expressed at the site of placentation at Day 30 of gestation as observed for EMMPRIN. Expression of MMP-1 or -9 mRNA was very low and was below the detection limit in the cyclic and pregnant endometrium.ConclusionEMMPRIN from the luminal epithelium may regulate the expression of stromal MMP-2 and -14 suggesting its crucial role in adhesion and fusion of embryo to luminal epithelium by directly itself through physiological tissues remodeling and developmental process, and/or stimulating MMPs to compensate endometrial functions.

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“…Young 1‐month‐old female rats were treated hormonally to simulate pregnancy conditions. The hormonal treatment consisted of successive injections of 25 international units (IU) of human follicle‐stimulating hormone and 48 hr later 25 IU of human chorionic gonadotropin (hCG) (McDonnel Smedts & Curry, ). The day following administration of hCG was considered Day 1 of pseudopregnancy, and oviducts ( n = 4) were collected 72 hr posttreatment (SP3).…”

Section: Methodsmentioning

confidence: 99%

Embryo presence regulates NODAL/LEFTY2 system in the rat oviduct in vivo

Argañaraz

Zampini

Apichela

et al. 2019

Molecular Reproduction Devel

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To gain further insight in the mechanisms of the embryo–maternal dialog in the oviduct, expression of members of the transforming growth factor‐β superfamily, NODAL, its inhibitor, LEFTY2, and their coreceptor, CFC1, were studied in the oviduct of 3‐day post copula (3 dpc) females with and without embryos (E and NE), pseudopregnant rats (SP3), and in 3‐day embryos. Nodal transcripts in SP3 oviducts showed a steady‐state relative abundance when compared with proestrus stage and the 3 dpc. In contrast, Lefty2 and Cfc1 relative abundance levels in proestrus and 3 dpc were higher. When comparing E with NE oviducts, Nodal and Lefty2 expression levels decreased, while Cfc1 expression increased in the presence of embryos. Nodal messenger RNA (mRNA) was observed in the embryo, but Lefty2 and Cfc1 transcripts were not found. In addition, an increase in Lefty2 expression coincided with increased levels of matrix metalloproteinases 9 mRNA and protein in the oviduct and in the oviductal fluid, respectively. These observations have shed new light on the relevance of the NODAL/LEFTY2 pathway in the oviduct during early embryo development and the role of the embryo in modulating this pathway.

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Expression of Basigin, an Inducer of Matrix Metalloproteinases, in the Rat Ovary1

Cited by 18 publications

References 41 publications

Expression of basigin in reproductive tissues of estrogen receptor-α or -β null mice

Expression of basigin in reproductive tissues of estrogen receptor-α or -β null mice

Expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and its related extracellular matrix degrading enzymes in the endometrium during estrous cycle and early gestation in cattle

Embryo presence regulates NODAL/LEFTY2 system in the rat oviduct in vivo

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