Expression of basigin in reproductive tissues of estrogen receptor-α or -β null mice

Abstract

Basigin plays important roles in both male and female reproduction because basigin (Bsg) null male and female mice are infertile. The aim of the present study was to determine whether basigin expression in reproductive organs requires oestrogen receptor (ER) α or ERβ. Expression of basigin protein in the testis, ovary and male and female reproductive tracts was studied in adult wild type, ERα-null (αERKO) and ERβ-null (βERKO) mice by immunohistochemistry and immunoblotting. Basigin mRNA levels in ovary and uterus were examined by quantitative RT-PCR. In females, basigin protein expression was observed mainly in granulosa and interstitial cells of the ovary and epithelial cells of the proximal oviduct in all genotypes. Basigin protein was also expressed in the uterine epithelium at prooestrus and oestrus in WT and βERKO mice but not in αERKO mice. However, a higher level of basigin mRNA was observed in uteri of αERKO mice compared with WT and βERKO mice. In males, basigin was expressed in Leydig cells and all germ cells except spermatogonia in all genotypes. Basigin was present in epithelial cells lining the efferent ductules in WT and βERKO mice but expression was greatly reduced in αERKO mice. In epididymal ducts, basigin expression was observed in epithelial cells in the caput and cauda in all genotypes. These data suggest that expression of basigin protein requires ERα, but not ERβ, in the uterus and efferent ductules, but is independent of ER in the ovary, oviduct, testis and epididymis.

CD147, CD44, and the Epidermal Growth Factor Receptor (EGFR) Signaling Pathway Cooperate to Regulate Breast Epithelial Cell Invasiveness

“…Also, although we saw differences in protein levels of CD147 with MEK inhibition and in our comparison of 10A-EV with 10A-K-Ras V12 cells, negligible changes in the mRNA level of CD147 were observed in either case, suggesting that the influence of Ras-MAPK signaling on CD147 expression occurs mainly at a post-transcriptional level. Similar post-transcriptional regulation of CD147 has been observed in earlier studies (83)(84)(85)(86)(87). Thus, although CD147 expression is clearly increased in most aggressive cancers, identification of CD147 as a differentially expressed message in microarray analyses may not always be apparent.…”

Sex Hormones Regulate Rat Hepatic Monocarboxylate Transporter Expression and Membrane Trafficking

“…Previous studies have evaluated the role of estrogen receptors (ER) in CD147 regulation in reproductive tissues utilizing knock out mice, with ERα dependent regulation in the uterus and efferent ducts, and ER-independent regulation in other tissues (21). There is a paucity of information in the literature on sex hormonemediated regulation of CD147 in tissues involved in drug disposition.…”

Sugar‐coated sperm: Unraveling the functions of the mammalian sperm glycocalyx

“…BSG is a transmembrane glycoprotein containing two extracellular immunoglobulin domains and a short cytoplasmic tail (Biswas et al, ; Toole, ). In the male, Bsg is expressed in spermatogonia, spermatocytes, spermatids, Sertoli cells, and Leydig cells (Bi et al, ), as well as throughout the epididymis–albeit at levels lower than in the testis (Chen et al, ). Bsg ‐knockout male mice are infertile due to the absence of mature sperm in the testis or epididymis.…”