Expression of AHPND toxin genes (pirAB), quorum sensing master regulator gene (luxR) and transmembrane transcriptional regulator gene (toxR) in Vibrio parahaemolyticus and Vibrio harveyi during infection of Penaeus vannamei (Bonne, 1931)

Muthukrishnan, Sarmila; Shariff, Mohamed; Yasin, Ina Salwany Md; Yusoff, Fatimah Md.; Ikhsan, Natrah Fatin Mohd

doi:10.1016/j.aquaculture.2022.737895

Cited by 7 publications

(4 citation statements)

References 48 publications

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“…It has been proven that the overabundance of certain bacterial community may have impacted the disease structure of the host and trigger the changes in the health status of the gut microbiota (Xiong et al, 2015). Furthermore, Vibrio species, such as V. harveyi, V. parahaemolyticus, V. alginolyticus, V. owensii and V. anguillarum were reported as common pathogenic bacteria in various marine animals (Lee et al, 2015;Chen et al, 2017;Deng et al, 2020;Deris et al, 2020;Zhang et al, 2020;Liao et al, 2022;Muthukrishnan et al, 2022). In fact, an overabundance of Vibrio species was found to be intimately related to the progression of shrimp, lobster, and crab diseases such as AHPND, Vibrio-caused bacteraemia, shell disease, luminescent vibriosis and septic hepatopancreatic necrosis disease (Diggles et al, 2000;Vogan et al, 2002;Jayasree et al, 2006;Nie et al, 2017;Morales-Covarrulbias et al, 2018;Xia et al, 2018;Prachumwat et al, 2020;Nurhafizah et al, 2021;Lioa et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Understanding the effects of salinity and Vibrio harveyi on the gut microbiota profiles of Litopenaeus vannamei

Deris

Iehata

Gan³

et al. 2022

Front. Mar. Sci.

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Increasing evidence have revealed a positive correlation between gut microbiota and shrimp health, in which a healthy shrimp gut consists of a complex and stable microbial community. Given that both abiotic and biotic factors constantly regulate shrimp gut microbiota, any changes affecting the levels of these factors could cause modification to the gut microbiota assemblage. The goal of this study was to explore the effects of salinity levels and pathogenic Vibrio harveyi infection on the diversity, structure, composition, interspecies interaction, and functional pathways of Litopenaeus vannamei gut microbiota. Juvenile shrimp were cultured at 5 ppt, 20 ppt, and 30 ppt for two months prior to Vibrio harveyi infection. After pathogenic V. harveyi challenge test, genomic DNA was isolated from the shrimp gut, and subjected to the 16S rRNA metagenomic sequencing analysis. We observed that gut microbiota diversity of shrimp cultured at 5 ppt and 30 ppt were lower than those cultured at 20 ppt after exposure to V. harveyi infection, suggesting that shrimp cultured at the two former salinity levels were more susceptible to V. harveyi infection. Network analysis also showed that shrimp cultured at 20 ppt exhibit a more stable bacterial network with complex interspecies interaction, even after induced by V. harveyi. Moreover, the presence of a high number of beneficial bacteria such as Pseudoruegeria, Rhodovulum, Ruegeria, Shimia and Lactobacillus in shrimp cultured at 20 ppt might have played a role in inhibiting the growth of V. harveyi and other potentially pathogenic bacteria. Besides, bacterial functional pathway prediction has also shown that metabolic pathways such as phenylalanine metabolism, glycine, serine and threonine metabolism, starch and sucrose metabolism, glyoxylate and dicarboxylate metabolism, carbon metabolism and biofilm formation process were significantly higher in shrimp cultured at 20 ppt. Collectively, our results suggested that 20 ppt is an optimal salinity that suppresses the growth of V. harveyi and potential pathogenic bacteria in the shrimp gut, which could possibly minimize the risk of pathogenic infection for sustainable production of healthy shrimp.

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Section: Discussionmentioning

confidence: 99%

Understanding the effects of salinity and Vibrio harveyi on the gut microbiota profiles of Litopenaeus vannamei

Deris

Iehata

Gan³

et al. 2022

Front. Mar. Sci.

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“…They have found that death rate is directly correlated with the expression level of virulence genes. This implies that QS‐related genes of pathogens are expressed inside the host 83 …”

Section: Impact Of Qs On Hostmentioning

confidence: 99%

“…This implies that QS-related genes of pathogens are expressed inside the host. 83 An interesting fact is that host can detect QS compounds as well, however, the receptors and signalling processes involved are unknown. QS molecules bind to AhR (aryl hydrocarbon receptor) of…”

Section: Impact Of Qs On Hostmentioning

confidence: 99%

Quorum sensing signals: Aquaculture risk factor

Priya

Boopathi

Murugan

et al. 2022

Reviews in Aquaculture

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Bacteria produce several virulence factors and cause massive mortality in fish and crustaceans. Abundant quorum sensing (QS) signals and high cell density are essentially required for the production of such virulence factors. Although several strategies have been developed to control aquatic pathogens through antibiotics and QS inhibition, the impact of pre‐existing QS signals in the aquatic environment has been overlooked. QS signals cause detrimental effects on mammalian cells and induce cell death by interfering with multiple cellular pathways. Moreover, QS signals not only function as a messenger, but also annihilate the functions of the host immune system which implies that QS signals should be designated as a major virulence factor. Despite QS signals' role has been well documented in mammalian cells, their impact on aquatic organisms is still at the budding stage. However, many aquatic organisms produce enzymes that degrade and detoxify such QS signals. In addition, physical and chemical factors also determine the stability of the QS signals in the aqueous environment. The balance between QS signals and existing QS signals degrading factors essentially determines the disease progression in aquatic organisms. In this review, we highlight the impact of QS signals on aquatic organisms and further discussed potential alternative strategies to control disease progression.

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“…Bacterial infections are one of the most serious risks to the global sustainability of aquaculture, causing significant economic loss. Acute hepatopancreatic necrosis disease is one of the most serious emerging diseases in shrimp‐producing countries (Lee et al, 2015; Muthukrishnan et al, 2022). This disease is caused by Vibrio bacteria and leads to high mortality by producing heavy peeling of hepatopancreatic cells, ultimately killing the shrimp.…”

Section: Introductionmentioning

confidence: 99%

Carbon sources improve water quality, microbial community, immune‐related and antioxidant genes expression and survival of challenged Litopenaeus vannamei Postlarvae in biofloc system

Hassan

Sharawy

El‐Nahas

et al. 2022

Aquaculture Research

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The influence of different processed agriculture wastes as carbon sources including sugarcane bagasse (SCB), rice bran (RB) and rice straw (RS) on water quality, microbial community, and expression of immune‐related and antioxidant genes in both hepatic and muscle tissues was evaluated. Furthermore, the cumulative mortality rates of Litopenaeus vannamei when challenged with Vibrio and Shewanella algae were estimated. The experiment lasted for 3 months in outdoor culture ponds, and the results indicated that the water quality parameters improved with different biofloc (BF) treatments. The total heterotrophic bacteria were increased in the BF treatments until the end of the experiment when compared to the control group. Regarding the hepatic and muscular expression of immune‐related genes such as beta‐glucan‐binding protein, lipopolysaccharides/b‐glucan‐binding protein, prophenoloxidase, lysozyme, crustin, transglutaminase, hemocyanin and antioxidant genes including glutathione peroxidase and superoxide dismutase, the SCB treatment was higher in all genes expression among different BF treatments and control. Moreover, the SCB treatment also had the best survival rate compared with RB, RS and control treatments when challenged with both Vibrio and Shewanella algae. In general, these results suggested that the SCB is an ideal carbon‐rich food source, boosts the immune system and promotes disease resistance of L. vannamei.

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Expression of AHPND toxin genes (pirAB), quorum sensing master regulator gene (luxR) and transmembrane transcriptional regulator gene (toxR) in Vibrio parahaemolyticus and Vibrio harveyi during infection of Penaeus vannamei (Bonne, 1931)

Cited by 7 publications

References 48 publications

Understanding the effects of salinity and Vibrio harveyi on the gut microbiota profiles of Litopenaeus vannamei

Understanding the effects of salinity and Vibrio harveyi on the gut microbiota profiles of Litopenaeus vannamei

Quorum sensing signals: Aquaculture risk factor

Carbon sources improve water quality, microbial community, immune‐related and antioxidant genes expression and survival of challenged Litopenaeus vannamei Postlarvae in biofloc system

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