Expression of adipokines and estrogen receptors in adipose tissue and placenta of patients with gestational diabetes mellitus

Abstract

To cite this version:P. Kleiblova, I. Dostalova, M. Bartlova, Z. Lacinova, I. Ticha, et al.. Expression of adipokines and estrogen receptors in adipose tissue and placenta of patients with gestational diabetes mellitus. Molecular and Cellular Endocrinology, Elsevier, 2009, 314 (1) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Page 1 of 36A c c e p t e d M a n u s c r i p t A c c e p t e d M a n u s c r i p t 2 SUMMARYThe purpose of this study was to assess the expression profile of genes with potential role in the development of insulin resistance (adipokines, cytokines/chemokines, estrogen receptors) in subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT) and placenta of pregnant women with gestational diabetes mellitus (GDM) and age-matched women with physiological pregnancy at the time of Caesarean section. qRT-PCR was used for expression analysis of the studied genes. Leptin gene expression in VAT of GDM group was significantly higher relative to control group. Gene expressions of interleukin-6 and interleukin-8 were significantly increased, whereas the expressions of genes for estrogen receptors α and β were significantly reduced in SAT of GDM group relative to controls, respectively. We found no significant differences in the expression of any genes of interest (LEP, RETN, ADIPOR1, ADIPOR2, CD68, ERα, ERβ) in placentas of women with GDM relative to controls. We conclude that increased expression of leptin in visceral adipose depot together with increased expressions of proinflammatory cytokines and reduced expressions of estrogen receptors in subcutaneous fat may play a role in the etiopathogenesis of GDM.

Expression of Adiponectin Receptors in Human Placenta and Its Possible Implication in Gestational Diabetes

“…The statistically significant increases in the maternal age, pre-pregnancy weight, BMI and birth weight of GDM women are in agreement with those published by American Diabetes Association (2009) which considered that a maternal age >25 years, increased weight and BMI before pregnancy are risk factors for GDM. Both types of receptors (ADIPOR1 and ADIPOR2) are present in human placenta in both fetal and maternal sides of GDM cases and normal pregnant women and these findings were in agreement with those of Meller et al (2006); Chen et al (2006) and Kleiblova et al (2010) while Caminos et al (2005) demonstrated the presence of only ADIPOR2 in human and rat placenta. ADIPOR1 is more highly expressed than ADIPOR2 in both fetal and maternal sides of GDM cases and normal pregnant women.…”

Impairment of insulin receptor signal transduction in placentas of intra-uterine growth-restricted newborns and its relationship with fetal growth

“…The relationship of resistin with total and activated IR content, and the correlations of adiponectin with IR content, activated IRS1, IRS2 and JNK suggest that both peptides play a role in placenta in regulating insulin action, and are in line with published reports (34)(35)(36). Findings are both in agreement with previous data (5), and at variance with recent findings in placenta of women with gestational diabetes (37).…”

Stężenie interleukiny-6, receptora dla interleukiny-6 i glikoproteiny 130 oraz cytokin zależnych od limfocytów Th17 u pacjentek z cukrzycą ciążową

“…Similarly, Lappas et al [33] did not find any marked differences in IL-6 release from the placenta, adipose tissue and skeletal muscle of the women with GDM compared to the NGT subjects. In contrast, Kleiblova et al [28] demonstrated higher IL-6 gene expression in subcutaneous adipose tissue from the patients with GDM. Furthermore, the causal relationship between IL-6, insulin resistance and GDM seems far from clear.…”

“…In the last few years, it has been suggested that the presence of GDM is connected with decreased oestrogen receptors expression [28] and abnormal adipokines production by adipose tissue [29], elevated expression of adenosine receptors in leukocytes [30], and an imbalance between circulating pro-and anti-inflammatory cytokines [20][21][22][23], but their contribution to the pathogenesis of GDM remains unclear.…”