Expression of active recombinant human lactoferrin in the milk of transgenic goats

Lactoferrin: structure, function and applications

“…Using microinjection and direct infection with viral vectors in the mammary gland, transgenic animals have been created that produce milk with rLF. These animals include goats [105], mice [107], rabbits [108] and cows [109], with expression levels of up to 2 g of hLF/L of milk in transgenic goats. Table 2 also shows the expression systems in plants where hLF expression has been attained; in rice, 1.6 mg of protein per gram of seed was produced.…”

Production of recombinant proteins in milk of transgenic and non-transgenic goats

“…Transgenic goats produced by the pronuclear microinjection by Zhang et al (2008) secreted elevated levels of human LTF in milk. In addition, the biochemical and physico-chemical characteristics of recombinant human LTF were similar to those of the native protein.…”

Animal agriculture and the importance of agnostic governance of biotechnology

“…Without treatment, infection is almost always fatal. African swine fever pigs [47] Avian influenza chickens [48] Avian leukosis virus chickens [49] Bacteria resistance sheep/pigs/fish [50][51][52][53] Bovine spongiform encephalopathy cattle, sheep [54,55] Bovine tuberculosis cattle [56] Foot and mouth disease various [57] Grass carp haemorrhage virus (GCHV) fish [58] Influenza A & classical swine fever pigs [59] Mastitis/health of nursing offspring various [60][61][62][63][64][65] Porcine reproductive and respiratory syndrome virus (PRRSV) pigs [66] Trypanosomiasis various [30] Visna virus resistance sheep [67] Losses in livestock production due to African animal trypanosomiasis (AAT), or nagana, are estimated at US $1 billion annually [24]. Direct losses in meat production and milk yield, plus the costs for trypanosomiasis control programs, are estimated at up to US $1.2 billion each year.…”