Expression of a WIPK-Activated Transcription Factor Results in Increase of Endogenous Salicylic Acid and Pathogen Resistance in Tobacco Plants

Waller, Frank; Müller, Axel; Chung, Kwi-Mi; Yap, Yun‐Kiam; Nakamura, Kimiyo; Weiler, Elmar W.; Sano, Hiroshi

doi:10.1093/pcp/pcj079

Cited by 24 publications

(17 citation statements)

References 23 publications

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“…A stress-responsive multifunctional protein involved in b b-oxidation in tobacco plants DNA and RNA blot hybridization was carried out under the standard method as described (Waller et al 2006). Subcellular localization was examined by fusion protein with GFP.…”

confidence: 99%

“…Healthy leaves from approximately 2 month-old plants were detached, and subjected to stress and chemical treatments. Briefly, wounding was given by punching leaves with a paper puncher; and TMV inoculation was performed by the temperature-shift method (Waller et al 2006). For chemical treatments, detached leaves were kept in a culture box with a cotton pad containing 10 ml of 100 mM jasmonic acid methyl ester (MeJA), or sprayed with 200 mM salicylic acid (SA).…”

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confidence: 99%

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A stress-responsive multifunctional protein involved in .BETA.-oxidation in tobacco plants

Ohya

Ogata

Nakamura

et al. 2008

Plant Biotechnology

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Biotic and abiotic-induced wounding is one of the severest stresses that plants suffer throughout the growing period. Upon injury, plants rapidly activates a set of genes, which encode diverse proteins to cope with damages. Screening such genes that were transcriptionally activated within 30 min after mechanical wounding in tobacco plants, we identified a particular clone encoding a multfunctional protein, and designated as NtMFP (Nicotiana tabacum multifunctional protein). The deduced polypeptide is constituted of 668 amino acids with an apparent molecular mass of 72.3 kDa, and bacterially expressed protein exhibited a clear b-oxidation activity. Fusion proteins with GFP were observed in cytosol, when expressed in onion epidermal cell layers. In addition to wounding, NtMFP transcripts were induced by tobacco mosaic virus infection, and by jasmonic acid treatments. When NtMFP was suppressed by the RNAi, transgenic tobacco showed dwarfism, early senescence and reduced expresstion of jasmonic acid-responsive genes. Multifunctional protein is generally considered to catalyze multiple steps of the fatty acid b-oxidation. It is also proposed to be involved in the b-oxidation step of jasmonic acid biosynthesis. The present results suggest the possibility that NtMFP commonly functions not only in fatty acid catabolism but also in jasmonic acid biosynthesis pathway.

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confidence: 99%

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confidence: 99%

A stress-responsive multifunctional protein involved in .BETA.-oxidation in tobacco plants

Ohya

Ogata

Nakamura

et al. 2008

Plant Biotechnology

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“…In the R11 transgenic line, lesions were visible at 24 h, but did not develop to the final stage even after 48 h, with dead tissues only around the lesion, forming ring-shaped spots (Fig. 8C) (37). Direct measurement of cell death by chloroplast autofluorescence indicated that, in the R131 and R71 transgenic plants, the hypersensitive response was much delayed in comparison with wild-type controls (Fig.…”

Section: Volume 281 • Number 46 • November 17 2006mentioning

confidence: 95%

Evolution of a Basic Helix-Loop-Helix Protein from a Transcriptional Repressor to a Plastid-resident Regulatory Factor

Kodama

Sano

2006

Journal of Biological Chemistry

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The tobacco gene NtWIN4 (Nicotiana tabacum wound-induced clone 4) is transiently up-regulated in response not only to wounding but also to pathogen attack. NtWIN4 encodes a putative basic helix-loop-helix protein with an apparent molecular mass of 28 kDa that exhibited clear nuclear transcription repression activity in Dual-Luciferase assays. However, immunoblotting indicated the existence of a 17-kDa form of NtWIN4 localized exclusively in tobacco leaf chloroplasts. Subsequent peptide dissection analyses with green fluorescent protein fusions revealed that a polypeptide of 81 amino acids starting at position 13 from the N terminus is maximally necessary for this localization. Further fine dissection analysis strongly suggested that the protein actually begins at the second Met located at position 27, yielding a signal peptide of 67 amino acids. However, the last C-terminal 15 amino acids overlap with the conserved basic region critical for DNA binding, so NtWIN4 presumably does not function as a transcription factor in planta. Transgenic tobacco plants constitutively overexpressing NtWIN4 demonstrated mortality with abnormal features, including albinism, and transient expression upon agroinfiltration resulted in distinct necrosis with a sharp decrease in chlorophyll content, consistent with the phenomenon known as chlorosis. Transgenic RNA interference tobacco plants exhibited reduced hypersensitive cell death, showing delayed tissue necrosis upon pathogen infection. These results suggest that NtWIN4 arose by divergence, becoming a chloroplast-resident factor from a nuclear transcriptional repressor by obtaining a transit peptide sequence, and that, upon translocation, it interacts with chloroplast components to induce hypersensitive cell death through chloroplast disruption, thereby contributing to plant stress responses.Higher plants are constantly exposed to diverse environmental stresses, including physical wounding. Under natural conditions, the major cause is grazing by herbivores, which continuously damages tissues, occasionally resulting in whole plant death after secondary infection by pathogens. To cope with this problem, plants have evolved a variety of self-defense mechanisms, including production of proteins such as proteinase inhibitors and of signaling components such as jasmonate and systemin (1-3). Wound signals are quickly transmitted from damaged cells to the whole plant, allowing elaboration of a defense reaction by transcriptionally up-and/or down-regulating a number of genes (4). Examples are proteinase inhibitors against insect feeding, extensions for reinforcement of cell walls, phenylalanine ammonia-lyase (EC 4.3.1.5) and chalcone synthase (EC 2.3.1.74) for phenylpropanoid synthesis, and 1-aminocyclopropane-1-carboxylate synthase and aminocyclopropane-1-carboxylate oxidase for ethylene synthesis (1, 5, 6). However, their functions have not been completely clarified.One of the phenotypic hallmarks of wounding is tissue necrosis. Cells at damaged areas rapidly die, thereby forming a physi...

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“…Transgenic NtWIF-overexpressing plants were prepared as previously described (Waller et al 2006), and RNAi plants were constructed using the pKANNIBAL (Wesley et al, 2001) vector designed for producing hairpin RNA with a loop. For sense orientation, the coding region of NtWIF1 cDNA was amplified by PCR using forward (5Ј-TATCTAGATGTCATTTCCTCAGGGTCA-AC-3Ј) and reverse (5Ј-TAGGATCCTTCCAAAACAGGGTG-GAATCTC-3Ј) primers.…”

Section: Plant Materialsmentioning

confidence: 99%

“…NtWIF transcripts were rapidly induced by wounding and pathogen attack. Its over-expression in tobacco transgenic plants conferred an elevated level of salicylic acid (SA), resulting in a constitutive expression of PR-1a and PR-2 under nonstressed conditions, and in rapid development of the hypersensitive response (HR) to exhibit resistance to pathogen attack (Waller et al 2006). These results linked WIPK with a transcription factor, thereby fulfilling the last step of MAPK cascade.…”

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Control of seed and root development by WIPK-activated transcription factor, NtWIF in tobacco plants

Chung

Kim

Sano

2007

Plant Biotechnology

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Expression of a WIPK-Activated Transcription Factor Results in Increase of Endogenous Salicylic Acid and Pathogen Resistance in Tobacco Plants

Cited by 24 publications

References 23 publications

A stress-responsive multifunctional protein involved in .BETA.-oxidation in tobacco plants

A stress-responsive multifunctional protein involved in .BETA.-oxidation in tobacco plants

Evolution of a Basic Helix-Loop-Helix Protein from a Transcriptional Repressor to a Plastid-resident Regulatory Factor

Control of seed and root development by WIPK-activated transcription factor, NtWIF in tobacco plants

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