A stress-responsive multifunctional protein involved in .BETA.-oxidation in tobacco plants

Ohya, Hitoshi; Ogata, Ayako; Nakamura, Kimiyo; Chung, Kwi-Mi; Sano, Hiroshi

doi:10.5511/plantbiotechnology.25.503

Cited by 6 publications

(2 citation statements)

References 18 publications

(20 reference statements)

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“…MFP2 is disrupted, showed impairment in wound-induced JA accumulation and defensive gene expression (Delker et al, 2007). In tobacco a similar result was obtained, that is, a stressresponsive MFP orthologous to Arabidopsis AIM1 are involved in β-oxidation (Ohya et al, 2008). Among five KAT genes in Arabidopsis, KAT2 was shown to play a major role in driving wound-activated responses by participating in the biosynthesis of JA in wounded leaves (Castillo et al, 2004).…”

Section: β-Oxidation Enzymessupporting

confidence: 58%

Jasmonate Biosynthesis, Perception and Function in Plant Development and Stress Responses

Yan¹,

Borrego²,

Kolomiets³

2013

Lipid Metabolism

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Section: β-Oxidation Enzymessupporting

confidence: 58%

Jasmonate Biosynthesis, Perception and Function in Plant Development and Stress Responses

Yan¹,

Borrego²,

Kolomiets³

2013

Lipid Metabolism

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“…A full-length cDNA corresponding to PhCHD was obtained by 5′-RACE and found to encode a protein of 724 amino acids with a calculated molecular mass of 78.1 kDa. This protein contains one hydratase and two dehydrogenase domains, and is 75 and 58% identical to Arabidopsis AIM1 and MFP2 (21,22), respectively, 69% identical to MFP from Oryza sativa (24), 65% identical to MFP from Nicotiana tabacum (25), and 60% identical to MFP-b from Cucumis sativus (26).…”

Section: Gene Encoding a Multifunctional Protein Is Highly Expressed mentioning

confidence: 99%

Completion of the core β-oxidative pathway of benzoic acid biosynthesis in plants

Qualley¹,

Widhalm²,

Adebesin

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

164

136

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Despite the importance of benzoic acid (BA) as a precursor for a wide array of primary and secondary metabolites, its biosynthesis in plants has not been fully elucidated. BA formation from phenylalanine requires shortening of the C 3 side chain by two carbon units, which can occur by a non–β-oxidative route and/or a β-oxidative pathway analogous to the catabolism of fatty acids. Enzymes responsible for the first and last reactions of the core BA β-oxidative pathway (cinnamic acid → cinnamoyl-CoA → 3-hydroxy-3-phenylpropanoyl-CoA → 3-oxo-3-phenylpropanoyl-CoA → BA-CoA) have previously been characterized in petunia, a plant with flowers rich in phenylpropanoid/benzenoid volatile compounds. Using a functional genomics approach, we have identified a petunia gene encoding cinnamoyl-CoA hydratase-dehydrogenase ( PhCHD ), a bifunctional peroxisomal enzyme responsible for two consecutively occurring unexplored intermediate steps in the core BA β-oxidative pathway. PhCHD spatially, developmentally, and temporally coexpresses with known genes in the BA β-oxidative pathway, and correlates with emission of benzenoid volatiles. Kinetic analysis of recombinant PhCHD revealed it most efficiently converts cinnamoyl-CoA to 3-oxo-3-phenylpropanoyl-CoA, thus forming the substrate for the final step in the pathway. Down-regulation of PhCHD expression in petunia flowers resulted in reduced CHD enzyme activity, as well as decreased formation of BA-CoA, BA and their derived volatiles. Moreover, transgenic lines accumulated the PhCHD substrate cinnamoyl-CoA and the upstream pathway intermediate cinnamic acid. Discovery of PhCHD completes the elucidation of the core BA β-oxidative route in plants, and together with the previously characterized CoA-ligase and thiolase enzymes, provides evidence that the whole pathway occurs in peroxisomes.

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Identification and characterization of differentially expressed genes from tobacco roots after decapitation

Wang

et al. 2011

Acta Physiol Plant

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Nicotine is the major alkaloid in tobacco and its biosynthesis is regulated by a variety of factors. Topping, as an important agronomic factor, can induce the nicotine biosynthesis increase. Some key nicotine biosynthesisrelated genes and the framework of nicotine biosynthesis pathway have been well studied, but the details of nicotine biosynthesis pathway are not well understood now. To investigate the genes expressed after tobacco topping, we constructed a suppression subtractive hybridization library using cDNA from control tobacco plants as driver and those from topped tobacco plants as tester. The insert size of positive clones was 200-1,000 bp confirmed by PCR. After differential screening, 560 significantly differently expressed clones among 1,950 positive clones were acquired, sequenced and 273 high quality expressed sequence tags (ESTs) were acquired. The results of nucleotide blast homological analysis indicated that these ESTs mainly involved in alkaloid biosynthesis (4%), plant hormone metabolism (3%), signaling/transcription (18%), stress/ defense (32%), protein metabolism (9%), carbon metabolism (6%), other metabolism (15%) and function unknown (13%). The expression of selected genes was analyzed by reverse transcription polymerase chain reaction and RNA gel blot hybridization, and the result indicated that their transcription amount increased after tobacco topping. NtNAC-R1was in silico cloned, and the expression level of NtNAC-R1 increased at 12 and 24 h in tobacco roots after topping, which indicated that NtNAC-R1 may play an important role in the signal transduction after tobacco topping. In addition to many previously reported nicotine biosynthesis-related genes, some new genes, such as transcription factors related to nicotine biosynthesis/regulation and the members of plant hormone pathway, were discovered in our library. The results contribute new data to the list of possible candidate genes involved in nicotine biosynthesis and regulation.

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A stress-responsive multifunctional protein involved in .BETA.-oxidation in tobacco plants

Cited by 6 publications

References 18 publications

Jasmonate Biosynthesis, Perception and Function in Plant Development and Stress Responses

Jasmonate Biosynthesis, Perception and Function in Plant Development and Stress Responses

Completion of the core β-oxidative pathway of benzoic acid biosynthesis in plants

Identification and characterization of differentially expressed genes from tobacco roots after decapitation

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