Expression of a quantitative character radius incompletus,  temperature effects, and localization of a mobile genetic  element Dm-412 in Drosophila melanogaster

VASILYEVA, L. A.; ZABANOV, S. A.; RATNER, V. A.; ZHIMULEV, I. F.; PROTOPOPOV, M. O.; BELYAEVA, E. S.

doi:10.1051/gse:19880201

Cited by 4 publications

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“…This suggested that passage to cell culture could trigger transposition, and reoriented the interest in cultured cells as an experimental system for the study of induction of transposition. The background for this interest is the body of facts and speculations on the ability of various environmental stresses to induce transposition or putative intermediates of transposition in plants and plant cultured cells (McClintock, 1984;Doting & Starlinger, 1986;Cullis, 1990), yeast (Paquin & Williamson, 1988;Rolfe et aL, 1986), mammals (Weinstein et al, 1988) and Drosophila Vasilyeva et al, 1988;Strand & McDonald, 1985;McDonald et al, 1988;Georgiev et al, 1990). …”

Section: Discussionmentioning

confidence: 99%

Evidence for De Novo rearrangements of Drosophila transposable elements induced by the passage to the cell culture

Franco¹,

Pisano

Peronnet

et al. 1992

Genetica

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The genomic distribution and the number of elements of eleven transposon families have been compared by the Southern technique between permanent cultured cells, larval salivary glands and the brains and whole flies of an inbred Drosophila line (inb-c) from which the cells were established. In cultured cells, changes in restriction patterns consistent with various types of rearrangements such as amplification, transposition and excision of the elements of copia, 1731, 412, 297 and mdg-4 transposon families are detected whereas B 104, G and blood elements appear stable. In previous reports these rearrangements were not detected among individuals of the inb-c line or among samples of somatic tissues, or in samples spanning years of maintenance of cultured cells. Hence, we believe that they have been induced de novo during the passage to the cell culture.

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Section: Discussionmentioning

confidence: 99%

Evidence for De Novo rearrangements of Drosophila transposable elements induced by the passage to the cell culture

Franco¹,

Pisano

Peronnet

et al. 1992

Genetica

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“…2 and 3). In the experiments with HS of line N 49, there were 13 different positions, and only 6 positions among them coincided with transposition sites induced by the stepwise temperature treatment of the ric population temperature lines ric113 and ric149 (2,3). Perhaps these differences reflect the properties of different treatments.…”

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confidence: 99%

“…respectively, 3.4 x 10-2, 8.7 x 10-2, and <4.1 x 10-4 transpositions per genome per occupied position per generation. Therefore, as a result of heat shock treatment, the probabilities of transpositions were two orders of magnitude greater than those of the control sample in the next generation after induction.…”

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confidence: 99%

“…Vasilyeva and coworkers (1)(2)(3)(4)(5) discovered the effects of stepwise temperature treatment of Drosophila at specially sensitive periods of the pupal stage. The heritable changes of expression of the mutant radius incompletus (ri) were seen accompanied by the changed patterns of localization of copia-like MGEs.…”

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confidence: 99%

“…This hybridization was by the standard method (16) used earlier (2,3). DNA of MGE Dm412 (mdg-2) was from an original line of Finnegan et al (17) cloned in pBR 322 with a BamHI restriction site.…”

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Induction of the mobile genetic element Dm-412 transpositions in the Drosophila genome by heat shock treatment.

Ratner¹,

Zabanov²,

Kolesnikova³

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

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Males of a Drosophila melanogaster isogenic line with a mutation of the major gene for radius incompletus (ri) were treated by standard light heat shock (3TC for 90 min) and by heavy heat shock (transfer of males from 37C for 2 hr to 4rC for 1 hr and back; this procedure was repeated three times). In the F1 generation of treated males mated with nontreated females of the same isogenic line, mass transpositions of copia-like mobile genetic element Dm-412 were found. The altered positions ofthe element seem nonrandom; five "hot spots" of transposition were found. Probabilities of transpositions were estimated after light heat shock and heavy heat shock and in the control sample. These probabilities were, respectively, 3.4 x 10-2, 8.7 x 10-2, and <4.1 x 10-4 transpositions per genome per occupied position per generation. Therefore, as a result of heat shock treatment, the probabilities of transpositions were two orders of magnitude greater than those of the control sample in the next generation after induction. Comparison of the results with those after stepwise temperature treatment shows that the induction depends on the intensity of the stress action (temperature treatment) rather than on the type of the stress action. (7) and HS regulation sites (8, 9). After HS treatment of flies, transcription and transposition of different MGEs seemed to be activated (9-11).From this evidence, speculations and hypotheses have been advanced about the genetic system of HS regulation in relation to heritable temperature effects in Drosophila (4, 5, 10-12). There have also been theories about the evolutionary importance of the genome system of MGE genomic patterns after environmental stress (temperature, etc.) (4,5,(10)(11)(12).However, the data are incomplete. Vasilyeva and coworkers (1-3, 13) in most of their work used heterogeneous Drosophila lines, and in situ hybridization was done many tens of generations after temperature treatment. Indeed, in some repeated experiments this interval was decreased to several generations with the same results. But the possibility of selective influence was not excluded completely. Junakovic et al. (6) found the effects in the next generation, but their restriction fragments were not compared with patterns of MGE localization in polytene chromosomes.In this paper (see also ref. 14), we present data that demonstrate multiple transpositions of copia-like MGE Dm-412 in the next generation after HS treatment. This work was done with an isogenic Drosophila line to decrease pattern variability in the control sample and after treatment. MATERIALS AND METHODSIsogenic Lines. The isogenic lines with a mutation of the major gene for ri were created by using marked balanced chromosomes (15): M5 (X chromosome), Cy/Pm (chromosome 2), D/Sb (chromosome 3). Of 20 isogenic lines, one (N 49) was used for the HS treatment, being most stable in its pattern of MGE Dm412 localization and expression of the character ri.Control Sample. Young males and virgin females were collected from mass cultures of isog...

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Role of transposable elements in age-related genomic instability

Nikitin

Reis

1997

Genet. Res.

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Expression of a quantitative character radius incompletus, temperature effects, and localization of a mobile genetic element Dm-412 in Drosophila melanogaster

Cited by 4 publications

References 0 publications

Evidence for De Novo rearrangements of Drosophila transposable elements induced by the passage to the cell culture

Evidence for De Novo rearrangements of Drosophila transposable elements induced by the passage to the cell culture

Induction of the mobile genetic element Dm-412 transpositions in the Drosophila genome by heat shock treatment.

Role of transposable elements in age-related genomic instability

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