Expression of a novel gene encoding protease inhibitor from metagenome of sponge in Vietnam

Abstract

Marine sponge is known as a “gold mine” of natural products from marine environment. Many novel bioactive compounds have been isolated from marine sponges and sponge-associated microorganisms such as antibiotics, anti-cancer compounds, protease inhibitors, etc. In this study, we selected a gene encoding protease inhibitor from metagenome of a sponge collected in Da Nang to express in Escherichia coli BL21(DE3). The gene PI-DN9 encoding protease inhibitor (1.3 kb) was cut off cloning vector pUC57/PI-DN9 containing gene PI-DN9 and inserted into expression vector pET32a(+), the recombinant vector pET32a(+)/PI-DN9 then was transformed and expressed in the E. coli strain BL21(DE3). Results showed that recombinant protein (50 kDa) was expressed successfully at 25°C, 1 mM of IPTG in 5 hours. The recombinant protein was purified using Ni-NTA affinity chromatography column. Western blot assay and bioactive assay showed good activity of the purified protein.

Effect of postpartum depression on exclusive breast-feeding practices in sub-Saharan Africa countries: a systematic review and meta-analysis

“…By 2020, the World Health Organization projected that depression will become the second and significant predictor of the global burden of disease [ 56 ]. One in five women in low- and middle-income countries developed postpartum depression according to reviews conducted in Low and Middle Income Countries (LMICS) and a review conducted in Africa [ 57 , 58 ]. Evidence has shown that common mental disorders, including depression, during the postpartum period are more prevalent compared with non-pregnancy periods [ 59 ].…”

Effect of Postpartum Depression on Exclusive Breast-Feeding Practices in Sub-Saharan Africa Countries: Systematic Review and Meta-analysis

“…By 2020, the World Health Organization projected that depression will become the second and signi cant predictor of the global burden of disease (56). One in ve women in low-and middle-income countries developed postpartum depression according to reviews conducted in Low and Middle Income Countries (LMICS) and a review conducted in Africa (57,58). Evidence has shown that common mental disorders, including depression, during the postpartum period are more prevalent compared with nonpregnancy periods (59).…”