Magnetic Fe3O4nanoparticles were prepared by coprecipitation and then coated with silica. These Fe3O4/SiO2nanoparticles consisted of a 10–15 nm magnetic core and a silica shell of 2–5 nm thickness. The superparamagnetic property of the Fe3O4/SiO2particles with the magnetization of 42.5 emu/g was confirmed by vibrating sample magnetometer (VSM). We further optimized buffers with these Fe3O4/SiO2nanoparticles to isolate genomic DNA of hepatitis virus type B (HBV) and of Epstein-Barr virus (EBV) for detection of the viruses based on polymerase chain reaction (PCR) amplification of a 434 bp fragment ofSgene specific for HBV and 250 bp fragment of nuclear antigen encoding gene specific for EBV. The purification efficiency of DNA of both HBV and EBV using obtained Fe3O4/SiO2nanoparticles was superior to that obtained with commercialized Fe3O4/SiO2microparticles, as indicated by (i) brighter PCR-amplified bands for both HBV and EBV and (ii) higher sensitivity in PCR-based detection of EBV load (copies/mL). The time required for DNA isolation using Fe3O4/SiO2nanoparticles was significantly reduced as the particles were attracted to magnets more quickly (15–20 s) than the commercialized microparticles (2-3 min).
In this article, the thymol, one of phenolic derivative as color creative reagent and nitropruside as reaction catalyst were investigated for photometric and visual quick-test determination of ammonia in fresh and drinking water based on Berthelot (phenate) method. For this purpose, one test set should have maximum 2 to 3 mixed components solution. So the different solution preparation modes and reagents combination were studied. The investigation results showed that the 2 mixed solution mode was impossibly prepared because nitropruside was decomposed in strong oxidative solution such as hypochlorite and increased color intensity when mixed with thymol in strong alkaline solution by ageing. The 3 mixed solution mode was proposed and optimal components mixing was fount i. e. solution 1 is nitropruside in ammonia free water; solution 2 is hypochlorite in carbonate buffer at pH 10 and solution 3 is thymol and EDTA in 2M of sodium hydroxide. This 3 solution test set was used for ammonia quick determination. Following proposed test procedure, the full color creation takes less than 5 minutes and total time for one test is approximately 7-8 minutes. The ammonia concentration in different samples determined by photometric and visual method using 3 solution test set were compared with conventional spectrophotometric method. The measured values showed that analytical results were agreed each with other and the proposed test set could be used as test-kit for field measurement or common monitoring ammonia in fresh and drinking water. The maximum relative error is less than 25%.
Oceanic mesoscale eddies continuously regulate the horizontal and vertical transport of mass, heat, salt, carbon, and nutrients throughout the ocean system owing to their ubiquity, three-dimensionality, and long-term persistence. Although satellites have been the main platforms used to observe mesoscale eddies and chlorophyll-a (Chla) distributions, they cannot support submesoscale physical–biological interactions. Contemporary satellite observations of Eulerian velocity fields are unable to resolve submesoscale processes that govern vertical migration and mixing, which are crucial for controlling the nutrients and light for phytoplankton in the surface layer. We explored the physical–biological interaction between the anticyclonic mesoscale eddy and the Chla secondary bloom that occurred after the spring bloom in the East/Japan Sea using the Geostationary Ocean Color Imager (GOCI). The GOCI currents were generated using GOCI Chla data and were used to map streamlines, vorticity, and divergence to characterize the surface current near the eddy. In the early spring bloom period, the eddy interior showed Chla depletion as the eddy was trapped externally. We found that the second bloom period coincided with a higher divergence or upwelling period in the eddy core, and a sharp Chla peak was observed when wind-induced Ekman suction was pronounced. This study describes the first satellite observation of surface layer divergence inside an anticyclonic mesoscale eddy with internal Chla blooms, utilizing a submesoscale-permitting GOCI-based surface current.
Fe 60 Pd 40 nanoparticles were prepared by sonoelectrodeposition. After annealing at various temperatures from 450°C to 700°C, the nanoparticles were found to have an ordered L1 0 structure and to show hard magnetic properties. Among the samples investigated, the nanoparticles annealed at 600°C exhibited the highest coercivity which amounts to 2.31 kOe at 2 K and 1.83 kOe at 300 K.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.