Expression of a cystatin transgene can confer resistance to root lesion nematodes in Lilium longiflorum cv. ‘Nellie White’

Vieira, Paulo; Wantoch, Sarah; Lilley, Catherine J.; Chitwood, David J.; Atkinson, H. J.; Kamo, Kathryn

doi:10.1007/s11248-014-9848-2

Cited by 47 publications

(24 citation statements)

References 47 publications

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“…As an alternative to RNAi, Vieira et al (113) showed that overexpression of a cystatin OcI D86 transgene can confer resistance to P. penetrans in hairy roots of soybean and Lilium longiflorum plants, with a reduction in nematodes reproduction of 75%. For practical application, a combination of RNAi and expression of a cystatin or a peptide that disorients nematodes (107) might provide the most effective and durable transgenic resistance to RLNs.…”

Section: Gene Silencing In Root Lesion Nematodesmentioning

confidence: 99%

Advances in Understanding the Molecular Mechanisms of Root Lesion Nematode Host Interactions

Fosu-Nyarko

Jones

2016

Annu. Rev. Phytopathol.

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Root lesion nematodes (RLNs) are one of the most economically important groups of plant nematodes. As migratory endoparasites, their presence in roots is less obvious than infestations of sedentary endoparasites; nevertheless, in many instances, they are the major crop pests. With increasing molecular information on nematode parasitism, available data now reflect the differences and, in particular, similarities in lifestyle between migratory and sedentary endoparasites. Far from being unsophisticated compared with sedentary endoparasites, migratory endoparasites are exquisitely suited to their parasitic lifestyle. What they lack in effectors required for induction of permanent feeding sites, they make up for with their versatile host range and their ability to move and feed from new host roots and survive adverse conditions. In this review, we summarize the current molecular data available for RLNs and highlight differences and similarities in effectors and molecular mechanisms between migratory and sedentary endoparasitic nematodes.

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Section: Gene Silencing In Root Lesion Nematodesmentioning

confidence: 99%

Advances in Understanding the Molecular Mechanisms of Root Lesion Nematode Host Interactions

Fosu-Nyarko

Jones

2016

Annu. Rev. Phytopathol.

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“…Previously, Vieira et al . () identified in the transcriptome of P. penetrans a highly abundant transcript encoding for a FAR gene. Along with the in silico analyses performed in this study, three different FAR members of this family were identified in P. penetrans , and a molecular characterization of the Pp‐far‐1 gene is provided herein.…”

Section: Introductionmentioning

confidence: 99%

Characterization and silencing of the fatty acid‐ and retinol‐binding Pp‐far‐1 gene in Pratylenchus penetrans

2017

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Fatty acid‐ and retinol‐binding proteins (FARs) are unique to nematodes, and are implicated in a wide range of metabolic and parasitic related functions. Along with the in silico analyses performed in this study, three different FAR members of this family were identified in Pratylenchus penetrans. The cDNA corresponding to the fatty acid‐ and retinol‐binding Pp‐far‐1 gene was cloned and herein characterized at the molecular level for the first time for this genus. The translated 185 amino acid sequence of P. penetrans FAR‐1 sequence, with a predicted molecular weight of 20.82 kDa and a pI of 5.49, shares highest sequence identity to FARs of other migratory nematodes of the Pratylenchidae family (90% to P. vulnus and 80% to Radopholus similis). In situ hybridization localizes Pp‐far‐1 transcripts in the hypodermis of the nematode. RT‐qPCR detected Pp‐far‐1 transcripts for all nematode developmental stages, with highest expression levels found in juveniles, adult females and adult males, respectively. Pp‐far‐1 is also highly expressed during infection and establishment of P. penetrans in roots of different host plants, such as corn, lily and soybean. The importance of Pp‐far‐1 was studied by in planta RNA interference (RNAi) assays using stable soybean hairy root lines. Targeting Pp‐far‐1 decreased expression of the nematode Pp‐far‐1 and significantly reduced the reproduction of nematodes, with a total of between 44% and 70% fewer nematodes in comparison to the average number of nematodes counted for control lines. The results indicate that suppressing the expression levels of Pp‐far‐1 can act as an effective target gene to control P. penetrans.

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“…Nellie White exhibited comparatively high resistant to an endoparasite, Pratylenchus penetrans causing RLN (root lesion disease). OcI Δ D86 expression inhibited parasitic effects of Pratylenchus by 75% in transgenic plants as compared to their wild relatives (Vieira et al, 2015; Table 4). …”

Section: Abiotic and Biotic Stress Tolerancementioning

confidence: 99%

Biotechnological Advancements for Improving Floral Attributes in Ornamental Plants

et al. 2017

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Developing new ornamental cultivars with improved floral attributes is a major goal in floriculture. Biotechnological approach together with classical breeding methods has been used to modify floral color, appearance as well as for increasing disease resistance. Transgenic strategies possess immense potential to produce novel flower phenotypes that are not found in nature. Adoption of Genetic engineering has supported the idea of floral trait modification. Ornamental plant attributes like floral color, fragrance, disease resistance, and vase life can be improved by means of genetic manipulation. Therefore, we witness transgenic plant varieties of high aesthetic and commercial value. This review focuses on biotechnological advancements in manipulating key floral traits that contribute in development of diverse ornamental plant lines. Data clearly reveals that regulation of biosynthetic pathways related to characteristics like pigment production, flower morphology and fragrance is both possible and predictable. In spite of their great significance, small number of genetically engineered varieties of ornamental plants has been field tested. Today, novel flower colors production is regarded as chief commercial benefit obtained from transgenic plants. But certain other floral traits are much more important and have high commercial potential. Other than achievements such as novel architecture, modified flower color, etc., very few reports are available regarding successful transformation of other valuable horticultural characteristics. Our review also summarized biotechnological efforts related to enhancement of fragrance and induction of early flowering along with changes in floral anatomy and morphology.

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Expression of a cystatin transgene can confer resistance to root lesion nematodes in Lilium longiflorum cv. ‘Nellie White’

Cited by 47 publications

References 47 publications

Advances in Understanding the Molecular Mechanisms of Root Lesion Nematode Host Interactions

Advances in Understanding the Molecular Mechanisms of Root Lesion Nematode Host Interactions

Characterization and silencing of the fatty acid‐ and retinol‐binding Pp‐far‐1 gene in Pratylenchus penetrans

Biotechnological Advancements for Improving Floral Attributes in Ornamental Plants

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