Expression of a carbonic anhydrase gene is induced by environmental stresses in Rice (Oryza sativa L.)

Song, Yu; Zhang, Xinxin; Guan, Qing; Takano, Tetsuo; Liu, Shenkui

doi:10.1007/s10529-006-9199-z

Cited by 53 publications

(33 citation statements)

References 17 publications

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“…Previous reports showed that the CA gene expression level and enzyme activity in triticale increased under salt stress, suggesting that CA played a part in the response to salt stress [19] . According to the examination findings of Yu et al, the similar result was also found in rice [20] . Fisher et al cloned the CA gene of strong salt tolerance from Dunaliella salina and found that the expression levels of mRNA and protein were enhanced along with the increase of the concentration of sodium chloride in the nutrient solution [21] .…”

Section: Introductionsupporting

confidence: 67%

Cloning of SjCA gene and its expression analysis on upland cottons

Kong

Zhao

et al. 2016

JBEI

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Acquisition of salt-tolerant genes from exogenous plants to improve cotton salt resistance has always been a hotspot of research on cotton salt resistance. However, the information regarding the method of conversion of living cotton pollens by portable gene gun technology is still little. Complete sequence information of the Carbonic Anhydrase (CA) gene was obtained from NCBI database, and its full ORF length sequence was cloned by RT-PCR technology. After the construction of pBI121-CA::GFP fusion expression vector, we used cotton pollens from upland cotton varieties Y-2067, ZA-23, and GZ-2, which have weaker autofluorescence, to conduct the research on the transient expression of cotton pollens in vivo via particle bombardment (gene gun technology). The results indicated that green fluorescence enhancement of the three kinds of cotton pollens was realized after the CA gene transformation, meaning that the CA gene expression level was increased. Besides, the salt tolerance germination ability of transgenic T1 seeds was also improved. Our research initially established a transient expression system of cotton pollen in vivo via particle bombardment technology, laying the theoretical foundation for further research of cotton genetic transformation and creation of cotton salt-tolerant germplasm.

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Section: Introductionsupporting

confidence: 67%

Cloning of SjCA gene and its expression analysis on upland cottons

Kong

Zhao

et al. 2016

JBEI

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“…Yu et al (2007) demonstrated that carbonic anhydrase gene was expressed in Oryza under environmental stress such as salt stress. Further, the root specifi c Contig68 showed a similarity with Aquaporin plasma membrane protein.…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis of microsatellite markers for salt stress in two contrasting maize parental lines and their RIL population

Yumurtaci

Sipahi

Zhao

2017

Acta Botanica Croatica

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-Salt stress considerably hinders the growth and productivity of maize (Zea mays L.). Identifi cation of salt tolerant genotypes and integration of alternative molecular markers have important roles in enhancing breeding processes. In this study, 3308 maize expressed sequence tags (ESTs) from salt stress-related libraries were assembled to mine repetitive sequences for development of applicable markers. In this core EST data, 208 simple and 18 non-simple repetitive regions were detected in 312 contigs and 1121 singletons. The di-nucleotide repeats were the most abundant type and accounted for 79.3%, followed by tri (19.7%), and tetra-nucleotide (1%). Among 59 EST-simple sequence repeats (SSRs), a total of 55 were screened for polymorphism between F35 (salt sensitive) and F63 (salt tolerant) parents and 48 out of 55 were detected as monomorphic. Signifi cantly, seven of them (12.7%) were found to be polymorphic and were used for genotyping of 158 F 5 derived recombinant inbred maize lines, and four of them were located on chromosome 1 and 3. Using in silico mapping, 44 out of 59 EST-SSR markers were mapped on 10 maize chromosomes. Analysis of sequence homology revealed different functional groups such as: membrane transport, cell defense, cell division, signaling components, photosynthesis and cell metabolism. These EST-SSRs might be used as new functional molecular markers in the diversity analysis, identifi cation of quantitative trait loci (QTLs) and comparative genomic studies in maize in the future.

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“…These results suggest that the expression of CA was related to environmental stresses. Transgenic Arabidopsis over-expressing OsCA1 had a greater salt tolerance at the seedling stage than wild-type plants and the CA activity was up-regulated by salts and osmotic stresses (Yu et al, 2007). The possible mechanism of over-expressing OsCA1 had a greater salt tolerance was that plants might rely on CA to catalyze HCO 3 -rapidly transformed to CO 2 in physiological drought condition caused by salt stress, to meet the physiology needs of the plants.…”

Section: The Application Of Gene Engineering About Higher Plant Casmentioning

confidence: 99%

“…Although the bulk of the CA activity is associated with the chloroplast fraction in C 3 plants, there are at least 10 to 15 % of the total activity is cytoplastic (Rumeau et al, 1996). Chloroplast CA contents in higher plants are saturated and cytoplasm CA contents are inadequate (Badger, 2003), and it is important to study the function and application of cytoplasm CAs in higher plant resistance because the CA activity was up-regulated by salts and osmotic stresses (Yu et al, 2007).…”

Section: General Enzymatic Characterization Of Higher Plant Casmentioning

confidence: 99%

Enzymatic Characteristics of Higher Plant Carbonic Anhydrase and Its Role in Photosynthesis

Sun¹,

Wu²,

Zhen-Zhen³

et al. 2014

JPS

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Carbonic anhydrase, a metalloenzyme which catalyses the reversible interconversion of HCO 3 − and CO 2 , is a major protein component of higher plant tissues. It is now shown that DNA sequence and encoded proteins for the different , β, ɑ γ, δ, ε, and ζ forms of carbonic anhydrases are present in living organisms. While there are no sequence homologies between the different type of carbonic anhydrases, all the , β, and ɑ γ carbonic anhydrase gene family enzymes in higher plants catalyze the same chemical reaction. Its specific function is generally assumed to convert CO 2 to HCO 3 − for the phosphoenolpyruvate carboxylase reaction and convert HCO 3 − to CO 2 for the ribulose-1,5-bisphosphate carboxylase reaction in photosynthesis. Moreover, carbonic anhydrase activity in guard cells is required for CO 2 -mediated stomatal regulation and carbonic anhydrases may provide an approach for plant alternatively protection against stress conditions. Recent studies on carbonic anhydrases described in this manuscript include the characterization and gene family of carbonic anhydrases, physiological function of higher plant CAs, and gene engineerings about higher plant CAs.

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Expression of a carbonic anhydrase gene is induced by environmental stresses in Rice (Oryza sativa L.)

Cited by 53 publications

References 17 publications

Cloning of SjCA gene and its expression analysis on upland cottons

Cloning of SjCA gene and its expression analysis on upland cottons

Genetic analysis of microsatellite markers for salt stress in two contrasting maize parental lines and their RIL population

Enzymatic Characteristics of Higher Plant Carbonic Anhydrase and Its Role in Photosynthesis

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