Genetic analysis of microsatellite markers for salt stress in two contrasting maize parental lines and their RIL population

Abstract

-Salt stress considerably hinders the growth and productivity of maize (Zea mays L.). Identifi cation of salt tolerant genotypes and integration of alternative molecular markers have important roles in enhancing breeding processes. In this study, 3308 maize expressed sequence tags (ESTs) from salt stress-related libraries were assembled to mine repetitive sequences for development of applicable markers. In this core EST data, 208 simple and 18 non-simple repetitive regions were detected in 312 contigs and 1121 singletons. The di-nucleotide repeats were the most abundant type and accounted for 79.3%, followed by tri (19.7%), and tetra-nucleotide (1%). Among 59 EST-simple sequence repeats (SSRs), a total of 55 were screened for polymorphism between F35 (salt sensitive) and F63 (salt tolerant) parents and 48 out of 55 were detected as monomorphic. Signifi cantly, seven of them (12.7%) were found to be polymorphic and were used for genotyping of 158 F 5 derived recombinant inbred maize lines, and four of them were located on chromosome 1 and 3. Using in silico mapping, 44 out of 59 EST-SSR markers were mapped on 10 maize chromosomes. Analysis of sequence homology revealed different functional groups such as: membrane transport, cell defense, cell division, signaling components, photosynthesis and cell metabolism. These EST-SSRs might be used as new functional molecular markers in the diversity analysis, identifi cation of quantitative trait loci (QTLs) and comparative genomic studies in maize in the future.

Molecular analysis of the F4 progenies obtained through pollen selection for heat tolerance in maize (Zea mays)

“…One hundred eighty SSR primers were used to screen two parental lines BTM4 and BTM6 out of which 16 primers (8.89%) were found to be polymorphic among parents. The extent of polymorphism observed in the present study was lower and found similar to earlier studies in maize (Yumurtaci et al 2017). Together for all the 16 primers, the homozygous male, homozygous female and heterozygotes were determined for GGG, GCG and CCC groups of inbred Hence, the combination of gametophytic selection and conventional sporophytic selection should be considered as an efficient tool in population improvement programs to achieve higher levels of tolerance to biotic and abiotic stresses relatively in short time.…”

Skrining Jagung (Zea mays L.) Toleran Salinitas Berdasarkan Karakter Pada Fase Awal Pertumbuhan

“…Tabel 1 menunjukkan bahwa salinitas menurunkan kemampuan berkecambah pada semua genotipe. Hasil tersebut sejalan dengan penelitian sebelumnya (Carpici et al, 2009;Sukma et al, 2018). Penurunan kemampuan perkecambahan juga terjadi pada tanaman sorghum (Almodares et al, 2007) dan kedelai (Blanco et al, 2007) Author(s): Kelik Perdana Windra Sukma; Qurrotul Aini __________________________________________ Pada air yang tidak mengandung garam, biji dapat berkecambah dengan baik karena proses imbibisi berjalan dengan baik.…”

Germination and Seedling Growth of Hybrid and Madura Local Corn on Salt Stress

“…Indonesia has shorelines 106 thousand kilometers and land potential 1,06 million hectares [5] It can be planted maize, but the main challenge the high content of salt will decrease productivity. Salinity is one of the main limiting factors that can decrease growth and productivity of maize [6] and many plant species [7] Corn var. Duko and Elos are Madura local corn which has the highest resistance to salinity [8].…”