Expression, Localization, and Binding Activity of the Ezrin/Radixin/Moesin Proteins in the Mouse Testis

Wakayama, Tomohiko; Nakata, Hiroki; Kurobo, Miho; Sai, Yoshimichi; Iseki, Shoichi

doi:10.1369/jhc.2008.952440

Cited by 21 publications

(21 citation statements)

References 52 publications

(65 reference statements)

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“…After washing in phosphate buffer at 4˚C, the specimens were postfixed in 1% osmium tetraoxide for 2 h at 4˚C, washed repeatedly in distilled water, stained with 1% uranyl acetate for 30 min, dehydrated through a graded ethanol series and propylene oxide, and embedded in Glicidether (Selva Feinbiochemica, Heidelberg, Germany). Ultrathin sections were cut and mounted onto copper grids, stained with 1% uranyl acetate for 10 min and with Reynolds lead citrate for 5 min, and evaluated using an H7650 electron microscope (Hitachi, Tokyo, Japan) (25).…”

Section: Methodsmentioning

confidence: 99%

Extravasated platelet aggregation in the livers of rats with drug-induced hepatic sinusoidal obstruction syndrome

Hirata

Tajima

Miyashita

et al. 2017

Molecular Medicine Reports

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Abstract. Oxaliplatin-based chemotherapy plays an important role in the treatment of colorectal liver metastases. Oxaliplatin, however, causes sinusoidal obstruction syndrome (SOS), which is characterized by portal hypertension, splenomegaly, thrombocytopenia, and liver dysfunction. SOS is diagnosed histopathologically by disruption of the sinusoidal endothelium, collagen deposition, fibrosis especially around zone 3, dilatation of the sinusoidal space and congestion. This study assessed the characteristics of a rat model of SOS. SOS was induced in rats by administration of monocrotaline (MCT). Blood chemistries and macroscopic and microscopic findings were compared in rats administered MCT and vehicle (control group). Levels of expression in the liver of CD41, P-selectin, rat endothelial cell antigen-1, CD34, and cleaved caspase-3 were analyzed immunohistochemically. Moreover, livers of these rats were analyzed by electron microscopy. Macroscopically, MCT-treated rats showed accumulation of bloody ascites and blue liver and were diagnosed with SOS histologically. Serum concentrations of aspartate aminotransferase (P=0.003), alanine aminotransferase (P=0.008), total-bilirubin (P=0.012), direct-bilirubin (P=0.007), indirect-bilirubin (P=0.003), lactate dehydrogenase (P<0.001) and hyaluronic acid (P=0.016) were significantly higher, and platelet counts significantly lower (P=0.004), in MCT-treated than in control rats. The livers of MCT-treated rats were immunohistochemically positive for CD41 and P-selectin, suggesting platelet aggregates; for rat endothelial cell antigen-1 and CD34, suggesting sinusoidal endothelial disorder; and for cleaved caspase-3, suggesting hepatocyte apoptosis. Electron microscopic findings revealed platelet aggregation in the space of Disse in the MCT group. Extravasated platelet aggregation in Disse's space may be involved in the development of SOS.

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Section: Methodsmentioning

confidence: 99%

Extravasated platelet aggregation in the livers of rats with drug-induced hepatic sinusoidal obstruction syndrome

Hirata

Tajima

Miyashita

et al. 2017

Molecular Medicine Reports

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“…Also, each of these four proteins has a unique function even they do share common functions (Table 1). Ezrin, radixin and moesin are found in the mouse testis, with ezrin associated with residual bodies, phagosomes and apical ES in the seminiferous epithelium [79]. Moreover, the expression of ezrin at the ES in the seminiferous epithelium of rat testes is highly stage-specific [80].…”

Section: Actin Binding Proteins (Abps)mentioning

confidence: 99%

Actin binding proteins in blood–testis barrier function

Mruk

Cheng

2015

Current Opinion in Endocrinology, Diabetes &Amp; Obesity

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Purpose of review This review examines the role of actin binding proteins (ABPs) on blood-testis barrier (BTB), an androgen-dependent ultrastructure in the testis, in particular their involvement on BTB remodeling during spermatogenesis. Recent findings The BTB divides the seminiferous epithelium into the basal and the adluminal compartments. The BTB is constituted by coexisting actin-based tight junction (TJ), basal ectoplasmic specialization (ES) and gap junction (GJ), as well as intermediate filament-based desmosome (DS) between Sertoli cells near the basement membrane. Junctions at the BTB undergo continuous remodeling to facilitate the transport of preleptotene spermatocytes residing in the basal compartment across the immunological barrier during spermatogenesis. Thus, meiosis I/II and post-meiotic spermatid development take place in the adluminal compartment behind the BTB. BTB remodeling also regulates exchanges of biomolecules between the two compartments. Since TJ, basal ES and GJ use F-actin for attachment, actin microfilaments rapidly convert between their bundled and unbundled/branched configuration to confer BTB remodeling. The events of actin re-organization are regulated by two major classes ABPs that confer actin microfilaments into bundled versus branched/unbundled configuration. Summary We provide a model on how ABPs regulate BTB remodeling, shedding new lights in unexplained male infertility, such as environmental toxicant-induced reproductive dysfunction.

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“…Capping protein β3 is a component of the cytoskeleton calyx of the mammalian sperm head (von Bülow et al, 1997). Wakayama et al (2009) showed in mouse testis that radixin immunoreactivity was expressed in the apical cytoplasm of Sertoli cells from 1 wk through 2 wk postpartum and that radixin was bound to both actin and tubulin. Evidence indicated that radixin is involved in the maturation of Sertoli cells, through interaction with different sets of membrane proteins and cytoskeletal components.…”

Section: Effects On Cytoskeleton and Its Regulationmentioning

confidence: 99%

In Utero Exposure to Environmentally Relevant Concentrations of PCB 153 and PCB 118 Disrupts Fetal Testis Development in Sheep

Krogenæs

Ropstad

Gutleb

et al. 2014

Journal of Toxicology and Environmental Health, Part A

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Polychlorinated biphenyls (PCB) are environmental pollutants linked to adverse health effects including endocrine disruption and disturbance of reproductive development. This study aimed to determine whether exposure of pregnant sheep to three different mixtures of PCB 153 and PCB 118 affected fetal testis development. Ewes were treated by oral gavage from mating until euthanasia (d 134), producing three groups of fetuses with distinct adipose tissue PCB levels: high PCB 153/low PCB 118 (n = 13), high PCB 118/low PCB 153 (n = 14), and low PCB 153/low PCB 118 (n = 14). Fetal testes and blood samples were collected for investigation of testosterone, testis morphology, and testis proteome. The body weight of the offspring was lower in the high PCB compared to the low PCB group, but there were no significant differences in testis weight between groups when corrected for body weight. PCB exposure did not markedly affect circulating testosterone. There were no significant differences between groups in number of seminiferous tubules, Sertoli cell only tubules, and ratio between relative areas of seminiferous tubules and interstitium. Two-dimensional (2D) gel-based proteomics was used to screen for proteomic alterations in the high exposed groups relative to low PCB 153/low PCB 118 group. Twenty-six significantly altered spots were identified by liquid chromatography (LC)-mass spectroscopy (MS)/MS. Changes in protein regulation affected cellular processes as stress response, protein synthesis, and cytoskeleton regulation. The study demonstrates that in utero exposure to different environmental relevant PCB mixtures exerted subtle effects on developing fetal testis proteome but did not significantly disturb testis morphology and testosterone production.

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Expression, Localization, and Binding Activity of the Ezrin/Radixin/Moesin Proteins in the Mouse Testis

Cited by 21 publications

References 52 publications

Extravasated platelet aggregation in the livers of rats with drug-induced hepatic sinusoidal obstruction syndrome

Extravasated platelet aggregation in the livers of rats with drug-induced hepatic sinusoidal obstruction syndrome

Actin binding proteins in blood–testis barrier function

In Utero Exposure to Environmentally Relevant Concentrations of PCB 153 and PCB 118 Disrupts Fetal Testis Development in Sheep

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