Expression Levels of <i>miR-30a-5p</i> in Papillary Thyroid Carcinoma: A Comparison Between Serum and Fine Needle Aspiration Biopsy Samples

İğci, Yusuf Ziya; Özkaya, Mesut; Korkmaz, Hakan; Bozgeyik, Esra; Bayraktar, Recep; Ulaşlı, Mustafa; Erkılıç, Suna; Eraydın, Ayten; Öztuzcu, Serdar

doi:10.1089/gtmb.2015.0062

Cited by 14 publications

(10 citation statements)

References 30 publications

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“…miR-30a-5p expression is induced by the Wnt/β-catenin pathway and promotes glioma cell invasion by inhibiting the expression of neural cell adhesion molecules ( 22 ). miR-30a-5p was significantly increased in serum samples and fine-needle aspiration biopsy samples from a group of patients with malignant PTC compared with patients with benign nodular goiters ( 23 ). miR-30a-5p was also demonstrated to be upregulated in the urine of patients with VSAD, and in VSAD tumor tissues and cell lines; following the surgical removal of VSAD, urinary miR-30a was distinctly reduced ( 24 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of the key miRNAs associated with survival time in stomach adenocarcinoma

et al. 2017

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Stomach adenocarcinoma (STAD) is one of the leading causes of cancer morbidity and mortality worldwide. The present study aimed to identify the microRNAs associated with STAD survival time. The clinical information and microarray miRNA and mRNA expression profiles of STAD patients were downloaded from The Cancer Genome Atlas. Differential expression (DE) analysis was performed to identify DEmiRNAs and DEmRNAs in STAD. The DEmiRNAs associated with the survival time of patients with STAD were identified through DE analysis, negative correlation pair analysis, miRNA target gene prediction, univariate Cox regression analysis and Kaplan-Meier analysis. The functions of the target genes of the DEmiRNAs were predicted with Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. A total of 355 DEmiRNAs and 1,722 DEmRNAs were identified between STAD and normal tissues. A total of 5 DEmiRNAs were identified to be associated with STAD survival, including 4 risk-associated DEmiRNAs (miR-30a, −143, −145 and −133b) and 1 protective DEmiRNA (miR-135b). The target DEmRNAs were significantly enriched for DNA metabolic process in the biological process GO category, and in KEGG cell cycle signaling pathways. Kaplan-Meier curves indicated that the overall survival time in the miR-30a, −143, −145 and −133b high expression groups was significantly shorter than that in the low expression groups, whereas the survival time was prolonged in the miR-135b high expression group compared with that in the low expression group. Therefore, miR-30a, −143, −145, −133b and −135b may be involved in the tumorigenesis and development of STAD, and may be potential biomarkers for its early diagnosis and prognosis.

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Section: Discussionmentioning

confidence: 99%

Identification of the key miRNAs associated with survival time in stomach adenocarcinoma

et al. 2017

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“…MiRNAs are involved in tumor proliferation, apoptosis, differentiation, invasion, and metastasis, as well as tumorigenesis [ 3 , 4 ]. MiRNAs dysregulation is a general feature in many cancers [ 5 ], such as glioma [ 6 ] and papillary thyroid carcinoma [ 7 ],; miR-30a expression is overexpressed but downregulated in lung cancer [ 8 , 9 ], giant cell tumor [ 10 ], breast cancer[ 11 – 15 ], renal cell carcinoma[ 16 ], hepatocellular carcinoma[ 17 , 18 ], colorectal cancer[ 19 , 20 ], ovary cancer[ 21 ], chondrosarcoma[ 22 ], gastric cancer[ 23 , 24 ], urothelial carcinoma of the bladder[ 25 ], nasopharyngeal carcinoma[ 26 ], Ewing tumor[ 27 ], pancreatic cancer[ 28 ], prostate cancer[ 29 ], and cervical cancer[ 30 ]. This data implies that the miR-30 family may play different roles as oncogenes or tumor suppressor genes depending on the type of cancer; similarly, miR-30a also plays an important role in cancer development and progression by modulating target genes, including inhibiting proliferation, invasion, and migration, inducing apoptosis.…”

Section: Introductionmentioning

confidence: 99%

MiR-30a: A Novel Biomarker and Potential Therapeutic Target for Cancer

Jiang

Zhang

Tang

2018

Journal of Oncology

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MicroRNAs (miRNAs) are small, highly conserved noncoding RNAs molecules, consisting of 18–25 nucleotides that regulate gene expression by binding to complementary binding sites within the 3′untranslated region (3′UTR) of target mRNAs. MiRNAs have been involved in regulating gene expression and diverse physiological and pathological processes. Several studies have reported that miR-30a, situated on chromosome 6q.13, is produced by an intronic transcriptional unit. Moreover, miR-30a has demonstrated its role in biological processes, including inhibiting proliferation and metastasis in many tumors, autophagy in chronic myelogenous leukemia, and regulating TGF-b1-induced epithelial-mesenchymal transition. However, based on the pathogenetic relationship between miR-30a and cancer in tumorigenesis, we believe that miR-30a may serve as tumor promising biomarker. Moreover, it would offer a therapeutic target for the treatment of cancer.

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“…Previous study shows that miR-30d promotes the apoptosis of renal cell carcinoma by modulating the FOXO/Akt pathway [16]. Although miR-30a-5p is involved in several types of cancers, including breast, liver, glioma, colon, and thyroid cancer, the expression and role of miR-30a-5p in ccRCC progression remains unclear [17-21]. …”

Section: Introductionmentioning

confidence: 99%

MicroRNA-30a-5p Inhibits the Growth of Renal Cell Carcinoma by Modulating GRP78 Expression

Wang

Cai

Liu

et al. 2017

Cell Physiol Biochem

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Background/Aims: MiR-30a-5p, a member of the microRNA-30 family (miR-30), is known to function as a tumor suppressor in several different cancers. However, the expression levels, biological function, and underlying mechanisms of miR-30a-5p in renal cell carcinoma (RCC) remain unclear. Glucose-regulated protein78 (GRP78) is a common cancer biomarker and promotes the growth and survival of cancer cells. The expression of GRP78 has been reported to be modulated by miR-30a in neurons. In this study, the expression profile of miR-30a-5p in clear cell renal cell carcinoma (ccRCC) and its effect on ccRCC through regulating GRP78 expression was investigated. Methods: MiR-30a-5p expression was analyzed using bioinformatic software on open microarray datasets from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO), and confirmed by quantitative RT-PCR (qRT-PCR) in ccRCC cell lines. Cell proliferation was investigated using CCK-8 and cell count assays. Western blotting, immunohistochemistry, luciferase reporter assays, and flow cytometry were employed to investigate the mechanisms of the effect of miR-30a-5p on ccRCC Results: MiR-30a-5p was down-regulated in ccRCC and related to the clinicopathological factors and prognosis of ccRCC. MiR-30a-5p was found to both suppress the growth of ccRCC cells and promote apoptosis of ccRCC cells in vitro. GRP78 was the direct target gene of miR-30a-5p, and the GRP78 expression was inversely correlated with the expression of miR-30a-5p in vivo and in vitro. The functional studies of GRP78 overexpression or knockdown demonstrated that GRP78 promoted proliferation and anti-apoptosis of ccRCC cells, and the oncogenic activity of GRP78 resulting in by miR-30a-5p overexpression. Conclusion: MiR-30a-5p is a bona fide negative regulator of GRP78 expression, and the anti-tumor activity of miR-30a-5p in ccRCC is due at least in part to down-regulating GRP78 expression and modulating the unfolded protein response (UPR) pathway. Thus, miR-30-GRP78 interaction provides a novel therapeutic candidate target in ccRCC treatment.

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Expression Levels of miR-30a-5p in Papillary Thyroid Carcinoma: A Comparison Between Serum and Fine Needle Aspiration Biopsy Samples

Abstract: The results of this study suggest that miR-30a-5p might be a novel diagnostic marker candidate in PTC. Further studies are required to investigate this possibility.

Cited by 14 publications

References 30 publications

Identification of the key miRNAs associated with survival time in stomach adenocarcinoma

Identification of the key miRNAs associated with survival time in stomach adenocarcinoma

MiR-30a: A Novel Biomarker and Potential Therapeutic Target for Cancer

MicroRNA-30a-5p Inhibits the Growth of Renal Cell Carcinoma by Modulating GRP78 Expression

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