Expression levels of estrogen receptor α mRNA in peripheral blood cells are an independent biomarker for postmenopausal osteoporosis

Chou, Chi-Wen; Chiang, Tsay-I; Chang, I-Chang; Huang, C.-H.; Cheng, Ya‐Wen

doi:10.1016/j.bbacli.2016.03.001

Cited by 8 publications

(5 citation statements)

References 22 publications

(28 reference statements)

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“…Recent studies have identified some biomarkers for osteoporosis. However, clinical applications of these biomarkers are limited by either their invasive nature or low diagnostic sensitivity and specificity [ 16 , 17 ]. In the present study, we identified plasma PTCSC3 upregulation in osteoporosis patients.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: LncRNA PTCSC3 is upregulated in osteoporosis and negatively regulates osteoblast apoptosis

Liu¹,

Chen²,

Liu

et al. 2022

BMC Med Genomics

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Background It is known that long non-coding RNA (lncRNA) PTCSC3 is involved in thyroid cancer and glioma, but its function in osteoporosis is unknown. The aim of our study was to investigate the role of lncRNA PTCSC3 in osteoporosis. Methods A total of 80 patients with osteoporosis (4 clinical stages) and four corresponding groups of healthy controls were enrolled. Plasma PTCSC3 levels in the 80 osteoporosis patients and 80 healthy volunteers were measured using RT-qPCR. The diagnostic potential of plasma PTCSC3 for osteoporosis was evaluated by ROC curve analysis with healthy volunteers as the true negative cases and corresponding osteoporosis patients as the true positive cases. Results PTCSC3 was upregulated in osteoporosis patients compared with healthy controls. PTCSC3 levels increased with osteoporosis stages increasing, but not with healthy controls aging. PTCSC3 overexpression separated each stage of osteoporosis from corresponding controls. PTCSC3 overexpression promoted while PTCSC3 silencing inhibited osteoblast apoptosis. However, PTCSC3 overexpression and silencing showed no significant effect on osteoclast apoptosis. LncRNA PTCSC3 was upregulated in osteoporosis and negatively regulated osteoblast apoptosis. Conclusion LncRNA PTCSC3 may serve as a potential therapeutic target for osteoporosis.

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: LncRNA PTCSC3 is upregulated in osteoporosis and negatively regulates osteoblast apoptosis

Liu¹,

Chen²,

Liu

et al. 2022

BMC Med Genomics

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“…Patients with OP develop reduced BMD and osteolysis, which is related to the imbalance of osteogenic differentiation and adipogenic differentiation in rBMSCs. ERα plays a more important role in OP than ERβ, and exerted bone-protective effects by controlling the activity of osteoblasts and osteoclasts (Chou et al, 2016). The ERα signaling axis also participates in osteoblast maturation (Lin et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Estrogen is an important hormone in the body, which is involved in various activities in many organisms. It is imperative for bone growth, development, and maintenance of bone health in adulthood (Chou, Chiang, Chang, Huang, & Cheng, 2016;Mandourah et al, 2018). Most estrogenic effects are mediated by the nuclear hormone receptor, which comprises estrogen receptor alpha and beta (ERα and ERβ; Novack, 2007).…”

Section: Introductionmentioning

confidence: 99%

MiR‐210‐3p inhibits osteogenic differentiation and promotes adipogenic differentiation correlated with Wnt signaling in ERα‐deficient rBMSCs

Peng

Zhu

et al. 2019

Journal Cellular Physiology

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MicroRNAs (miRNAs) regulate activities in living organisms through various signaling pathways and play important roles in the development and progression of osteoporosis.The balance between osteogenic and adipogenic differentiation of rBMSCs is closely related to the occurrence of osteoporosis. ERα regulates bone metabolism in various tissues. However, the correlation among ERα, miRNAs, and the differentiation of rBMSCs is still unclear. In this study, we used lentivirus transfection into rBMSCs to construct an ERα-deficient model, analyzed the differences in expressed miRNAs between control and ERα-deficient rBMSCs. The results revealed that the expression of 25 miRNAs were upregulated, 164 miRNAs were downregulated, and some of the regulated miRNAs such as miR-210-3p and miR-214-3p were related to osteogenic or adipogenic differentiation, as well as to particular signaling pathways. Next, we overexpressed miR-210-3p to evaluate its effects on the osteogenic and adipogenic differentiation of rBMSCs, and identified the relationship among miR-210-3p, Wnt signaling pathway, and the differentiation of rBMSCs. The results indicated that ERα-deficient inhibited osteogenic differentiation, promoted adipogenic differentiation, and regulated the expression of some miRNAs. Meanwhile, overexpression of miR-210-3p promoted osteogenic differentiation and inhibited adipogenic differentiation of rBMSCs, processes likely to be related to the Wnt signaling pathway. In conclusion, we identified a group of upregulated and downregulated miRNAs in ERα-deficient rBMSCs that might play a vital role in regulating osteogenic or adipogenic differentiation. One of these, miR-210-3p, inhibited osteogenic differentiation and promoted adipogenic differentiation correlated with the Wnt signaling pathway in ERα-deficient rBMSCs, providing new insight into the regulation of bone metabolism.

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“…However, its antagonistic Wnt signal increases with age [31]. On one hand, the decrease in estrogen will lead to an increase of osteoclasts and osteoclastic activity; on the other hand, it can promote the secretion of inflammatory factors, such as that of the interleukin and tumor necrosis factor (TNF) family, further promoting bone resorption, and affecting the balance of the RANKL-RANK-OPG axis, leading to increased osteoclast differentiation and activation of osteoclastic activity [32]. Rap1 is critical for resorptive function, and its selective inhibition in mature osteoclasts retards pathological bone loss [33].…”

Section: Discussionmentioning

confidence: 99%

Network Pharmacology-Based Pharmacological Mechanism of the Chinese Medicine Rhizoma drynariae Against Osteoporosis

Gan

Chen

et al. 2019

Med Sci Monit

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Rhizoma drynariae is the main traditional Chinese medicine used for the treatment of osteoporosis, but its anti-osteoporotic targeting mechanism has not been fully elucidated due to the complexity of its active ingredients. In this study, the pharmacological mechanism of action of Rhizoma drynariae against osteoporosis was studied by integrating pharmacological concepts. The pharmacokinetic characteristics of selected major active constituents of Rhizoma drynariae and the SMILES structural similarity were used to predict related targets. A literature search was conducted to identify known osteoporosis treatment targets, which were then combined with the predicted targets to construct the direct or indirect target interaction network map of Rhizoma drynariae against osteoporosis. Finally, data on the key targets of the interactions, ranked according to relevant node parameters obtained through pathway enrichment analysis and screening of key targets and active ingredients of Rhizoma drynariae , were used to perform molecular docking simulation. We screened 16 active ingredients of Rhizoma drynariae , and 7 key targets with direct or indirect effects with a high frequency were obtained. These main pathways were found to play important roles in the PI3k-akt signaling pathway, osteoclast differentiation, Wnt signaling pathway, and estrogen signaling pathway. Molecular docking showed that most active ingredients of Rhizoma drynariae had strong binding efficiency with key targets. Based on network pharmacology, we conclude that Rhizoma drynariae plays an anti-osteoporotic role by directly or indirectly targeting multiple major signaling pathways and influencing the proliferation and differentiation of multiple types of cells.

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Expression levels of estrogen receptor α mRNA in peripheral blood cells are an independent biomarker for postmenopausal osteoporosis

Cited by 8 publications

References 22 publications

RETRACTED ARTICLE: LncRNA PTCSC3 is upregulated in osteoporosis and negatively regulates osteoblast apoptosis

RETRACTED ARTICLE: LncRNA PTCSC3 is upregulated in osteoporosis and negatively regulates osteoblast apoptosis

MiR‐210‐3p inhibits osteogenic differentiation and promotes adipogenic differentiation correlated with Wnt signaling in ERα‐deficient rBMSCs

Network Pharmacology-Based Pharmacological Mechanism of the Chinese Medicine Rhizoma drynariae Against Osteoporosis

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