MiR‐210‐3p inhibits osteogenic differentiation and promotes adipogenic differentiation correlated with Wnt signaling in ERα‐deficient rBMSCs

Abstract

MicroRNAs (miRNAs) regulate activities in living organisms through various signaling pathways and play important roles in the development and progression of osteoporosis.The balance between osteogenic and adipogenic differentiation of rBMSCs is closely related to the occurrence of osteoporosis. ERα regulates bone metabolism in various tissues. However, the correlation among ERα, miRNAs, and the differentiation of rBMSCs is still unclear. In this study, we used lentivirus transfection into rBMSCs to construct an ERα-deficient model, analyzed the differences in expressed miRNAs between control and ERα-deficient rBMSCs. The results revealed that the expression of 25 miRNAs were upregulated, 164 miRNAs were downregulated, and some of the regulated miRNAs such as miR-210-3p and miR-214-3p were related to osteogenic or adipogenic differentiation, as well as to particular signaling pathways. Next, we overexpressed miR-210-3p to evaluate its effects on the osteogenic and adipogenic differentiation of rBMSCs, and identified the relationship among miR-210-3p, Wnt signaling pathway, and the differentiation of rBMSCs. The results indicated that ERα-deficient inhibited osteogenic differentiation, promoted adipogenic differentiation, and regulated the expression of some miRNAs. Meanwhile, overexpression of miR-210-3p promoted osteogenic differentiation and inhibited adipogenic differentiation of rBMSCs, processes likely to be related to the Wnt signaling pathway. In conclusion, we identified a group of upregulated and downregulated miRNAs in ERα-deficient rBMSCs that might play a vital role in regulating osteogenic or adipogenic differentiation. One of these, miR-210-3p, inhibited osteogenic differentiation and promoted adipogenic differentiation correlated with the Wnt signaling pathway in ERα-deficient rBMSCs, providing new insight into the regulation of bone metabolism.

<p>Comparison of the Therapeutic Effect of Allogeneic and Xenogeneic Small Extracellular Vesicles in Soft Tissue Repair</p>

“…This might be related to the complexity of the regulatory mechanism of cell differentiation. We have known that many proteins 45,46 and a lot of miRNAs 25,[47][48][49][50][51] have been reported to participate in adipogenic differentiation. Similarly, for angiogenesis, both proteins [52][53][54][55][56][57] and miRNAs [58][59][60] played a regulatory role.…”

Osteoblastogenesis Alters Small RNA Profiles in EVs Derived from Bone Marrow Stem Cells (BMSCs) and Adipose Stem Cells (ASCs)

“…Among the 10 miRNAs that changed in both ASCs and BMSCs upon osteoblastic differentiation, miR-210-3p and miR-335-3p were down-regulated and had relatively higher expression than the other down-regulated miRNAs in ASCs and BMSCs ( Table 1 ). In our previous study, these two miRNAs were also down-regulated and acted as negative regulators of osteoblastogenesis [ 40 ] and other studies have supported a role for these two miRNAs in osteoblastogenesis [ 42 , 43 ]. Notably, most of the miRNAs with significantly differential expression were cell origin specific: 47 miRNAs were only differentially expressed in ASCs and 25 miRNAs only in BMSCs.…”

Aberrant Expressional Profiling of Known MicroRNAs in the Liver of Silver Carp (Hypophthalmichthys molitrix) Following Microcystin-LR Exposure Based on samllRNA Sequencing

“…Wnt signaling plays an important role in the early development of organ formation, tissue regeneration, animal embryos, and other physiological processes. If mutations in key proteins in this signaling pathway lead to abnormal activation, cancer may be induced [44,45]. In summary, target genes of differentially expressed miRNAs induced by MC-LR may be involved in alterations in insulin signaling, PPAR, and Wnt pathways, indicating that MC-LR-induced toxicity in the liver of silver carp might be related to these pathways.…”