Expression in Escherichia coli of urokinase antigenic determinants

Bollen, Alex; Glineur, Cécile; Delcuve, Geneviève P.; Loriau, R.; Herzog, Albert

doi:10.1016/0006-291x(81)90465-4

Cited by 3 publications

(1 citation statement)

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“…One clone, designated 9HlOEl1, recognizes HMW urokinase light chain, and has been used successfully to isolate urokinase in minute concentrations from human urine, cell harvest fluid, and an E. coli broth without requiring pre-concentration or dialysis steps. Since the affinity purification technique described here has been successful with a variety of biological fluids, it should also be useful in the purification of genetically engineered urokinase, which may soon be produced by recombinant organisms (18). It is important to note that 9H10Ell MAB could be used effectively to separate HMW urokinase from the LMW form.…”

Section: Discussionmentioning

confidence: 99%

Purification of Urokinase from Complex Mixtures Using Immobilized Monoclonal Antibody Against Urokinase Light Chain

Vetterlein¹,

Calton²

1983

Thromb Haemost

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SummaryThe preparation of a monoclonal antibody (MAB) against high molecular weight (HMW) urokinase light chain (20,000 Mr) is described. This MAB was immobilized and the resulting immunosorbent was used to isolate urokinase starting with an impure commercial preparation, fresh urine, spent tissue culture media, or E. coli broth without preliminary dialysis or concentration steps. Monospecific antibodies appear to provide a rapid single step method of purifying urokinase, in high yield, from a variety of biological fluids.

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Section: Discussionmentioning

confidence: 99%